Survey of viruses infecting daphne in New Zealand

Forster, R. L. S.; Milne, K. S.

doi:10.1080/00288233.1975.10421066

Cited by 18 publications

(11 citation statements)

References 4 publications

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“…DVX is readily distinguished from other viruses found in daphne by particle size and morphology. It was the only virus found in daphnes which was almost exclusively confined to D. cneorum (Forster & Milne 1975). The virus was present in all nursery plants of D. cneorum tested and is thus spread by vegetative propagation.…”

Section: Discussionmentioning

confidence: 93%

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Daphne virus X: A potexvirus from daphne

Forster

Milne

1978

New Zealand Journal of Agricultural Research

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Daphne virus X (DVX) was found in all specimens of Daphne cneorum L. indexed for virus but was not associated with macroscopic symptoms. Experimental hosts of DVX included 18 species from 6 dicotyledonous families. Inl'ectivity was retained in expressed sap of Nicotiana clevelandii Gray for 5 weeks at c. 20°c. in sap diluted to 10-5 , after heating for 10 min at 80°c. Attempts to transmit DVX with Myzus persicae (Sulz) failed. DVX was purified lrom N. clevelandii leaves using chloroform: n-butanol clarification, one cycle of differential. then density-gradient, centrifugation. Preparations had a 260: 280 ratio of 1.22, a sedimentation coefficient of 110 S, and a buoyant density in CsCl of 1.28 g/m!. An antiserum to DVX had a titre of 1: 2048. DVX was not serologically related to clover yellow mosaic virus, narcissus mosaic virus, potato virus X, and white clover mosaic virus. The flexuous filamentous particles of DVX had a normal length of 499 nm in negatively stained preparations from LJ. cneorum flowers. Ultra-thin sections of pea leaflets systematically infected with DVX contained aggregates of DVX-like particles as well as unaggregated particles scattered in the cytoplasm. It is proposed that DVX is ::i distinct potexvirus. The present cryptogram for DVX is

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Section: Discussionmentioning

confidence: 93%

“…Methods for aphid culture, electron microscopy, analytical ultracentrifugation, and spectro-I,hotometry have been detailed elsewhere (Forster & Milne 1976).…”

Section: Methodsmentioning

confidence: 99%

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Daphne virus X: A potexvirus from daphne

Forster

Milne

1978

New Zealand Journal of Agricultural Research

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“…Methods for electron microscopy, analytical ultracentrifungation, and spectrophotometry were those detailed by Forster & Milne (1976).…”

Section: Methodsmentioning

confidence: 99%

Daphne virus S: A carlavirus from daphne

Forster

Milne

1978

New Zealand Journal of Agricultural Research

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Daphne virus S (DVS) was associated with leaf distortion and down-curling of leaf edges of Daphne odora Thunb. 'Lcucanthe' but was latent in other Daphne spp. and cultivars. DVS was transmitted from daphne to daphne by grafting and by Myzus persicae (Sulz.), but nttempts to sap transmit the virus to 20 different herbaceous test plants failed. The virus was purified from D. odora flowers by clarification with n-butanol, followed by differential and oucrose-gradient centrifugation. Preparations had a 260: 280 ratio of 1.35 and a sedimentation coefficient of 174 S. An antiserum to DVS had a titre of 1: 2048. DVS was distantly scrologically related to carnation latent virus and potato virus S but was serologically unrelatcd to three other carlaviruser and eight potyviruses. The straight to slight flexuous particles of DVS had normal lengths ranging from 704 to 716 nm.It is proposed that DVS is a distinct carlavirus. The present cryptogram for DVS is

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“…), but the rate of transmission of the virus is increased by grafting (Foster and Milne 1978). DVS was reported to be the most common virus found infecting daphne plants in New Zealand in 1975 (Foster andMilne 1975). According to the survey, 11 viruses were detected from the daphne plant in New Zealand; namely, alfalfa mosaic virus (AMV), arabis mosaic virus (ArMV), cucumber mosaic virus (CMV), and seven other uncharacterized viruses, including DVS.…”

Section: Daphne Virus S (Dvs) Belongs To the Genus Carlavirus In The mentioning

confidence: 99%

Complete Genome Sequence of a Japanese Isolate of <i>Daphne virus S</i>

et al. 2015

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Summary Daphne virus S (DVS), a member of the genus Carlavirus, was isolated from a daphne plant in Japan. The genome of the isolate (DVS-JP) consisted of 8736 nucleotides and was predicted to contain six open reading frames (ORFs). The overall genome sequence identity between DVS-JP and the previously reported Korean isolate of DVS (DVS-K) was 83.0%, whereas identities to other carlaviruses ranged from 20.5-56.0%. Each DVS-JP ORF shared more than 80% identity at both the nucleotide and amino acid levels with DVS-K, but less than 56% with other carlaviruses, which confirmed that DVS-JP was the same species as DVS-K. The RNA dependent RNA polymerase (RdRp) showed the highest identity with Phlox virus S, and the coat protein (CP) showed the highest identity with Helenium virus S and Chrysanthemum virus B. The genome sequence of an isolate of DVS-JP revealed a high degree of identity with DVS-K, which increases our understanding of the evolution of the genus Carlavirus.

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Survey of viruses infecting daphne in New Zealand

Cited by 18 publications

References 4 publications

Daphne virus X: A potexvirus from daphne

Daphne virus X: A potexvirus from daphne

Daphne virus S: A carlavirus from daphne

Complete Genome Sequence of a Japanese Isolate of <i>Daphne virus S</i>

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