Survey of Virulence Determinants among Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolated from Clinical Specimens of Hospitalized Patients of North west of Iran

Sharifi, Yaeghob; Hasani, Alka; Ghotaslou, Reza; Varshochi, Mojtaba; Hasani, Akbar; Aghazadeh, Mohammad; Milani, Morteza

doi:10.2174/1874285801206010034

Cited by 50 publications

(57 citation statements)

References 44 publications

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“…Gelatinase is a metalloprotease with a hydrolytic enzymatic required for biofilm formation which is encoded by gelE (17). The gelE gene was observed in 55% (32/63 E. faecalis and 15/22 E. faecium) of all isolates and as the second most pathogenic factor in our study and also 39% of MDR strains associated with this gene.…”

Section: Discussionsupporting

confidence: 57%

“…The results was indicated 18 out total eighty five isolates are related to MDRs, and the majority of MDR isolates were associated with E. faecium species (16,17). Recent studies also described a gradual increasing of MDR isolates throughout nosocomial infections (16,18).…”

Section: Discussionmentioning

confidence: 91%

“…However, recent investigation described different incidences in clinical isolates; for instance, Sabia et al reported that 70% of vancomycin resistance enterococci isolates carried the gelE; they also found gelatinase activity in E. faecalis species more than E. faecium. Nevertheless, Sharifi et al detected gelE with 7.9% of vancomycin resistance enterococci in Hospitalized Patients of Northwest of Iran (17,22). Previous studies have indicated the importance and frequency of gelatinase enzyme in enterococcal infections.…”

Section: Discussionmentioning

confidence: 99%

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Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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Background: The enterococci are responsible for infections including bacteremia and endocarditis which are usually resistant to multiple antibiotics. This nosocomial agent probably harbors putative virulence factors which increases their capability to colonize hospitalized patients. Objectives: This study was aimed in order to find the frequency of various virulence factors in enterococci and their relationship with multidrug resistance (MDR). Methods: The samples were collected from different hospital wards including; Intensive care unit (ICU), cardiac care unit (CCU), pediatrics department, internal wards, and transplantation. The isolates were detected by biochemical tests and in order to determine the antibiotic susceptibility pattern, disk diffusion agar (kirby-bauer) was accomplished. Then, MICs (Minimum inhibitory concentrations) of vancomycin were determined by E-test strips. For molecular examinations and detection of drug resistance genes, the simple polymerase chain reaction was used. The multiplex PCR was used in order to detect virulence factors. Results: Total of 85 isolates were obtained from one university teaching hospital in southeast of Iran. Approximately 95% of isolated which were from urine specimens and 34% of isolates were collected from pediatrics units at hospital. Tetracycline resistance (48%) has been observed with a high frequency and related to the tetM gene. Furthermore, eighteen isolates were recognized as MDR strains that carried vanA, aac (6)-Ie-aph (2)-Ia, ermB, and tetM genes. Among virulence factor genes, asa1 (69%) and gelE (55%) are more frequently observed in both species. In general, we found Enterococcus faecalis strains more prevalent. Also, E. faecium was related to antibiotic resistance genes in nosocomial infection. Conclusions:The data was indicated a high prevalence of multiple antibiotics resistance genes with virulence determinants in enterococci and also considered resistant isolate in pediatrics unit. The current results can be recommended in order to change new strategies for antibiotic therapy, because this serious pathogen is important for treatment and eradication in hospitals. Furthermore, the biofilm formation was regulated and constructed by virulence determinants that could be a candidate for enterococcal treatment.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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“…Furthermore, esp may mediate the interaction with primary surfaces and participate in biofilm formation which substantially increases bacterial survival in biopolymers and may also be involved in antimicrobial resistance (Ballering et al, 2009 andChuang-Smith et al 2010). Knowledge of the virulence characteristics of circulating Enterococcus strains may help to understand the complex pathogenic process of these opportunistic pathogens (Sharifi et al, 2012).Therefore, this study aimed to screening for genes encoding pathogenicity-associated factors for isolates of E. faecalis from UTI in Duhok city, in addition to investigate the antibiotics susceptibility patterns of the isolated strains.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq

Khalid

2016

SJUOZ

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ABSTRACT:Enterococcus faecalis is one of the leading causes of many infections and mainly urinary tract infections. This pathogen developed high resistance to multiple antibiotics and it harbor many virulence factors genes. This study aimed to determine the antibiotic resistance patterns and screening for some virulence factor genes of E. faecalis isolated from urinary tract infection. Urine samples were collected from 788 outpatient's clinic having clinical signs of UTI that visited Azadi Teaching Hospital in Duhok city. Urine samples were cultured on bacteriological media and isolated colonies identified using standard bacteriological methods. Antibiotic susceptibility was performed by Kirby Bauer test. All isolates were subjected to species-specific PCR assay for confirmatory identification followed by targeting virulence genes. Twenty five isolates of E. faecalis were detected and confirmed by species-specific PCR assay that expressed high antibiotic-resistance to many selected drugs except norfloxacin, penicillin and ampicillin. The most prevalent genes among all isolates were cpd genes followed by asa1, ace, esp, and gelE. Bearing of virulence genes combination were more frequent among multiple-antibiotic resistant strains. This study highlighted on E. faecalis as causes of UTI in Duhok city that showed multiple resistances to common antibiotics and harboring more than one virulence gene.

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“…In current study, our results for the frequency of enterococci in clinical samples in hospitals have similarity to other studies in this subject. 8,13,14 After identification of first VRE strain in early 1980, colonization and infectious diseases caused by these bacteria have been enhanced. Currently, these bacteria are important cause of nosocomial infections (NIs) and due to multidrug resistance features and treatment failures, the mortality rate in hospitalized patients are increased.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the Prevalence of vanA and vanB genes in vancomycin resistant enterococcus (VRE) by Taq Man real time PCR Assay

Mirzaei¹,

Farivar²,

Juhari³

et al. 2013

Jmid

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Objective: Enterococci are important nosocomial agents and due to their potential antimicrobial resistance they have a significant role in the dissemination of resistance genes. Currently, these species are described as healthcare concern. The aim of this study was to determine vanA and vanB genes in vancomycin resistant enterococci (VRE) strains isolated from the various clinical samples in the hospitals in Iran.Methods: Susceptibility of 235 strains to vancomycin was screened as minimum inhibitory concentration (MIC) by Etest. The genes encoding modifying vancomycin precursor's dipeptide termini named as vanA and vanB genes were targeted by Taq Man real time PCR assay in vancomycin resistant and vancomycin intermediate resistant Enterococcus faecalis and Enterococcus faecium strains.Results: A total of 235 enterococci were isolated from the clinical specimens. One hundred and ninety three (82.1%) of them were defined as E. faecalis, 33 (14.0%) E. faecium, 1/235 (0.4%) E. avium, 1/235 (0.4%) E. raffinosus and 7/235 (3.0%) E. galinarium. The prevalence of vancomycin resistance was 13.6% (32/235) consisting of 18/235 (7.7%) E. faecalis and 6.0% (14/235) E. faecium. Among the 32 VRE strains, a total of 36 vanA and vanB genes were detected (some isolates had both vanA and vanB genes). These resistance genes were not detected in 5 out of 32 (15.6%) isolates.Conclusion: E. faecalis was more common in clinical samples and vanA (58.3%) gene was the predominant gene among the VRE isolates. The current study showed that Taq Man real time PCR assay is the useful, precise and rapid detection of vancomycin resistance genes. J Microbiol Infect Dis 2013;3(4): 192-198 Mirzaei B, et al. Detection of vanA and vanB genes in VRE 193

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Survey of Virulence Determinants among Vancomycin Resistant Enterococcus faecalis and Enterococcus faecium Isolated from Clinical Specimens of Hospitalized Patients of North west of Iran

Cited by 50 publications

References 44 publications

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Molecular Detection of Virulence Factors of Enterococcus Faecalis Isolated From Urine Samples in Duhok City, Kurdistan Region/Iraq

Investigation of the Prevalence of vanA and vanB genes in vancomycin resistant enterococcus (VRE) by Taq Man real time PCR Assay

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