Survey of Vineyard Insects and Plants to Identify Potential Insect Vectors and Noncrop Reservoirs of Grapevine Red Blotch Virus

Wilson, H. F.; Hogg, Brian; Blaisdell, Kai G; Andersen, Jeremy C.; Yazdani, Armand S; Billings, Alexis C.; Ooi, Kei-Lin Murata; Soltani, Nourolah; Almeida, Rodrigo P. P.; Cooper, Monica L.; Rwahnih, Maher Al; Daane, Kent M.

doi:10.1094/phytofr-04-21-0028-r

Cited by 10 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spissistilus festinus is not an efficient vector of GRBV as transmission occurs at a low rate from infected Vvin to healthy Vvin [33,51] [this study]. In a previous study, the directional spread of GRBV was predicted to occur predominantly from vineyards hosting GRBV inoculum to free-living vines in riparian habitats [26].…”

Section: Discussionmentioning

confidence: 96%

“…Of the few vector candidates identified in a diseased 'Cabernet franc' vineyard in Napa County in northern California, Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae), the three-cornered alfalfa hopper, proved to be the most likely candidate [3] and was subsequently reported to vector GRBV [30,31]. Other hemipteran vector candidates were recently described in California [32,33].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transmission of Grapevine Red Blotch Virus by Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between Free-Living Vines and Vitis vinifera ‘Cabernet Franc’

Hoyle

Flasco

Choi

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

Grapevine red blotch disease emerged within the past decade, disrupting North American vine stock production and vineyard profitability. Our understanding of how grapevine red blotch virus (GRBV), the causal agent of the disease, interacts with its Vitis hosts and insect vector, Spissistilus festinus, is limited. Here, we studied the capabilities of S. festinus to transmit GRBV from and to free-living vines, identified as first-generation hybrids of V. californica and V. vinifera ‘Sauvignon blanc’ (Vcal hybrids), and to and from V. vinifera ‘Cabernet franc’ (Vvin Cf) vines. The transmission rate of GRBV was high from infected Vcal hybrid vines to healthy Vcal hybrid vines (77%, 10 of 13) and from infected Vvin Cf vines to healthy Vcal hybrid vines (100%, 3 of 3). In contrast, the transmission rate of GRBV was low from infected Vcal hybrid vines to healthy Vvin Cf vines (15%, 2 of 13), and from infected Vvin Cf vines to healthy Vvin Cf vines (19%, 5 of 27). No association was found between transmission rates and GRBV titer in donor vines used in transmission assays, but the virus titer was higher in the recipient leaves of Vcal hybrid vines compared with recipient leaves of Vvin Cf vines. The transmission of GRBV from infected Vcal hybrid vines was also determined to be trans-stadial. Altogether, our findings revealed that free-living vines can be a source for the GRBV inoculum that is transmissible by S. festinus to other free-living vines and a wine grape cultivar, illustrating the interconnected roles of the two virus hosts in riparian areas and commercial vineyards, respectively, for virus spread. These new insights into red blotch disease epidemiology will inform the implementation of disease management strategies.

show abstract

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Transmission of Grapevine Red Blotch Virus by Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between Free-Living Vines and Vitis vinifera ‘Cabernet Franc’

Hoyle

Flasco

Choi

et al. 2022

Viruses

Self Cite

View full text Add to dashboard Cite

show abstract

“…Surveys for GRBV in vegetation adjacent to cultivated vineyards in California have found alternate hosts growing in riparian edge habitats (Bahder et al 2016b, Wilson et al 2021. Thirteen species of woody herbaceous plants growing around three vineyards were tested for the presence of GRBV.…”

Section: Date Collectedmentioning

confidence: 99%

“…The Virginia creeper leafhopper (Erythroneura ziczac) successfully transmitted the virus under greenhouse conditions, but subsequent assays did not replicate these results (Poojari et al 2013, Bahder et al 2016a. Several leafhopper and treehopper species collected from V. vinifera vineyards in New York and California tested positive for GRBV DNA, but their ability to transmit the virus directly has not been determined (Cieniewicz et al 2018, 2019, Wilson et al 2021.…”

mentioning

confidence: 99%

Identification of Potential Grapevine Red Blotch Virus Vector in Missouri Vineyards

LaFond¹,

Volenberg²,

Schoelz³

et al. 2022

Am J Enol Vitic.

View full text Add to dashboard Cite

Grapevine red blotch virus (GRBV), the causal agent of grapevine red blotch disease, has been detected in vineyards across the United States and throughout Missouri. Insect transmission of GRBV in cultivated vineyards of Missouri has not been investigated previously. The objectives of this study were to characterize the potential insect vectors present in four commercial vineyards that had previously been determined to be infected with GRBV, test potential vectors caught in vineyards and surrounding habitats for the presence of GRBV with the use of PCR, and investigate the ability of candidate vectors to acquire and transmit GRBV using controlled greenhouse experiments. Of the vineyard-collected insects tested over the course of this research, one species of treehopper, Entylia carinata, tested positive for GRBV. This species and one other treehopper, Enchenopa binotata, were selected for direct transmission assays. Both species successfully acquired GRBV from infected grapevines and transmitted GRBV to confirmed GRBV-free grapevines. E. carinata has been identified as a promising economic vector after insect samples from vineyards tested positive for GRBV, and monitoring data placed this species as the secondmost abundant treehopper captured in traps. We do not consider E. binotata a likely economically significant vector because our monitoring data showed that this species was rare and only found along edge habitat surrounding vineyards, never inside vineyard rows. Samples of the most abundant treehopper, Micrutalis calva, have not tested positive, but its vector status remains unresolved. Further research on rates of secondary spread and transmission by M. calva are required, but these results provide evidence that insect transmission of GRBV is feasible in the region.

show abstract

“…GRBV is graft-transmissible [ 6 , 12 ] and the only known natural hosts of GRBV are Vitis spp. [ 12 , 13 , 14 ]. These include grape cultivars and rootstock genotypes [ 6 , 12 ], as well as free-living vines in Northern California [ 15 , 16 , 17 ] and Southern Oregon [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Distinct Red Blotch Disease Epidemiological Dynamics in Two Nearby Vineyards

Flasco

Cieniewicz

Pethybridge

et al. 2023

Viruses

View full text Add to dashboard Cite

Grapevine red blotch virus (GRBV) causes red blotch disease and is transmitted by the three-cornered alfalfa hopper, Spissistilus festinus. GRBV isolates belong to a minor phylogenetic clade 1 and a predominant clade 2. Spatiotemporal disease dynamics were monitored in a 1-hectare ‘Merlot’ vineyard planted in California in 2015. Annual surveys first revealed disease onset in 2018 and a 1.6% disease incidence in 2022. Ordinary runs and phylogenetic analyses documented significant aggregation of vines infected with GRBV clade 1 isolates in one corner of the vineyard (Z = −4.99), despite being surrounded by clade 2 isolates. This aggregation of vines harboring isolates from a non-prevalent clade is likely due to infected rootstock material at planting. GRBV clade 1 isolates were predominant in 2018–2019 but displaced by clade 2 isolates in 2021–2022, suggesting an influx of the latter isolates from outside sources. This study is the first report of red blotch disease progress immediately after vineyard establishment. A nearby 1.5-hectare ‘Cabernet Sauvignon’ vineyard planted in 2008 with clone 4 (CS4) and 169 (CS169) vines was also surveyed. Most CS4 vines that exhibited disease symptoms one-year post-planting, likely due to infected scion material, were aggregated (Z = −1.73). GRBV isolates of both clades were found in the CS4 vines. Disease incidence was only 1.4% in non-infected CS169 vines in 2022 with sporadic infections of isolates from both clades occurring via secondary spread. Through disentangling GRBV infections due to the planting material and S. festinus-mediated transmission, this study illustrated how the primary virus source influences epidemiological dynamics of red blotch disease.

show abstract

Survey of Vineyard Insects and Plants to Identify Potential Insect Vectors and Noncrop Reservoirs of Grapevine Red Blotch Virus

Cited by 10 publications

References 45 publications

Transmission of Grapevine Red Blotch Virus by Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between Free-Living Vines and Vitis vinifera ‘Cabernet Franc’

Transmission of Grapevine Red Blotch Virus by Spissistilus festinus [Say, 1830] (Hemiptera: Membracidae) between Free-Living Vines and Vitis vinifera ‘Cabernet Franc’

Identification of Potential Grapevine Red Blotch Virus Vector in Missouri Vineyards

Distinct Red Blotch Disease Epidemiological Dynamics in Two Nearby Vineyards

Contact Info

Product

Resources

About