Survey of the Incidence of Viral Infections in Calanthe spp. and Characterization of a GW Isolate of Cymbidium mosaic virus in Korea

Park, Chung Youl; Baek, D.-S.; Jeong-hak, Oh，; Choi, Jong Yoon; Bae, Dae Hyeon; Kim, Jeong Seon; Jang, Gil Hun; Lee, Su Heon

doi:10.5423/rpd.2016.22.2.65

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…5'-ACGATCATATTCATCGCATGG-3' (Park et al, 2016). The PCR reaction was employed, using a thermal PCR system (BioRad, MyCycler, USA) in a total volume of 25 µL.…”

Section: Rna Preparation and Rt-pcr Analysismentioning

confidence: 99%

“…Cymbidium mosaic virus (CymMV), which belongs to the genus Potexvirus, is the most pathogenic problem that causes the loss of orchid cultivation worldwide (Park et al, 2016;Yusop et al, 2022) (Lee et al, 2021). Phenotypically, the orchid plants infected by CymMV show mosaic, necrotic, and chlorotic symptoms, and imperfection of flower growth (Liu et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic Relationship of Cymbidium Mosaic Virus from the Native Orchids of South Kalimantan, Indonesia

Mursyidin,

Saputra

2023

J Bio Bio Edu

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Information on viral genetics, including their phylogenetic relationship, is valuable in controlling viral infection and screening for the development of virus-resistant cultivars in the future. The objectives of this study were to detect and characterize the Cymbidium mosaic virus (CymMV) from the native orchids of South Kalimantan, Indonesia, by the RT-PCR method. Also, to determine their phylogenetic relationship based on a partial genome of RdRp by the ML and PCA methods. Following RT-PCR analysis, one of 10 samples of native orchids used was positively infected by CymMV. In early detection, the RdRp region of CymMV has approximately 530 bp in size. After being sequenced and aligned with other isolates, this region has 121 polymorphic or mutation sites, a GC content of 45.21%, a transition/transversion bias value of 3.52, and nucleotide diversity (0.0415). The phylogenetic analysis revealed that CymMV from South Kalimantan, Indonesia, has closest related to similar isolates from Korea Type 2 (AF016914.1), Niigata, Japan (AB197937.1), Hawaii (EF125180.1), and Taiwan M2 (EU314803.1), with the coefficient divergence of 0.025. But, it has very distantly related to Hawaii 18-1 (EF125178.1) with a coefficient of 0.142. The results provide urgent information in supporting the native orchid's conservation and breeding efforts, locally and globally, including mitigating or controlling the viral infection and screening for the development of virus-free or resistant cultivars in the future.

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“…5'-ACGATCATATTCATCGCATGG-3' (Park et al, 2016). The PCR reaction was employed, using a thermal PCR system (BioRad, MyCycler, USA) in a total volume of 25 µL.…”

Section: Rna Preparation and Rt-pcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic Relationship of Cymbidium Mosaic Virus from the Native Orchids of South Kalimantan, Indonesia

Mursyidin,

Saputra

2023

J Bio Bio Edu

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Detection methods and in vitro elimination techniques for orchid viruses: A review

Das

Nongsiang

Sanglyne

2023

South African Journal of Botany

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Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid ‘New True’ plants via meristem-derived protocorm-like body (PLB) subcultures

Campol,

Naing,

Aung

et al. 2024

Plant Methods

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Background This study aimed to produce Odontoglossum ringspot virus (ORSV)-free Cymbidium orchid ‘New True’ plants from ORSV-infected mother plants by culturing their meristems and successively repeating subcultures of protocorm-like bodies (PLBs) derived from the meristems. Results Initially, ORSV was confirmed as the causative agent of viral symptoms in orchid leaves via reverse transcription-polymerase chain reaction (RT-PCR) analysis. Meristems from infected plants were cultured to generate PLBs, which in sequence were repeatedly subcultured up to four times. RT-PCR and quantitative RT-PCR analyses revealed that while ORSV was undetectable in shoots derived from the first subculture, complete elimination of the virus required at least a second subculture. Genetic analysis using inter-simple sequence repeat markers indicated no somaclonal variation between regenerated plants and the mother plant, suggesting that genetic consistency was maintained. Conclusion Overall, our findings demonstrate that subculturing PLBs for a second time is ideal for producing genetically stable, ORSV-free Cymbidium orchids, thus offering a practical means of generating genetically stable, virus-free plants and enhancing plant health and quality in the orchid industry. Supplementary Information The online version contains supplementary material available at 10.1186/s13007-024-01269-1.

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Survey of the Incidence of Viral Infections in Calanthe spp. and Characterization of a GW Isolate of Cymbidium mosaic virus in Korea

Cited by 4 publications

References 20 publications

Phylogenetic Relationship of Cymbidium Mosaic Virus from the Native Orchids of South Kalimantan, Indonesia

Phylogenetic Relationship of Cymbidium Mosaic Virus from the Native Orchids of South Kalimantan, Indonesia

Detection methods and in vitro elimination techniques for orchid viruses: A review

Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid ‘New True’ plants via meristem-derived protocorm-like body (PLB) subcultures

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