Survey of Plant-Parasitic Nematodes Associated with Yams in Ogun and Osun States of Nigeria

Adegbite, A. A.; Saka, J. O.; Agbaje, G. O.; Osuloye, Felix Oluwole

doi:10.2478/v10045-008-0051-4

Cited by 19 publications

(21 citation statements)

References 5 publications

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“…A number of Dioscorea species are also the source of phyto-compounds which are used in the production of corticosteroids and have potential uses as excipients in the pharmaceutical industry (Poornima and Ravishankar 2007;Zuluaga et al 2007;Okunlola and Odeku 2011). Yam production has been constrained owing to several abiotic and biotic factors that include diseases and pests, inadequate planting material, low yield potential, and decreasing soil fertility (Amusa et al 2003;Adegbite et al 2006). Despite the vitality of yam, its genetic improvement through conventional breeding has been hindered by the prolonged breeding cycle, dioecious and erratic flowering nature, poor seed setting, polyploidy, vegetative propagation through seed tubers and vine cuttings, and heterozygous genetic background (Mignouna et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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Dioscorea rotundata, commonly known as white yam, is an important staple food crop widely cultivated in West Africa and provides food security to millions of people. Genetic improvements of this crop using the advanced biotechnology tools have been hampered hitherto by the recalcitrant nature of regeneration through somatic embryogenesis. Therefore, we have developed an efficient and reproducible system for plant regeneration via somatic embryogenesis. Explants of different types (immature leaf, node, stem internode, root segment, petiole, and axillary bud) of D. rotundata accession TDr 2436 were tested for their embryogenic potentials on Murashige and Skoog (MS) medium supplemented with various auxins (2,4-D, NAA, and picloram). Among all explants tested, axillary bud explants cultured on MS medium supplemented with picloram (0.5-12 mg/l) favored the production of calli. Maximum proliferation of calli (526 mg fresh weight/explant) was achieved on MS medium supplemented with picloram (0.5 mg/l), casein hydrolysate (600 mg/l), and proline (1 g/l). Histology analysis confirmed that the embryogenic calli produced on this medium were mixed with non-embryogenic calli. Transfer of calli on MS basal medium supplemented with activated charcoal (1 %) changed the color of calli to purple and promoted the production of somatic embryos (87 embryos/callus) as well as adventitious shoot buds. Furthermore, upon transfer to MS medium supplemented with BAP (0.4 mg/l), the embryos continued their differentiation and maturation and germinated into complete plantlets. The adventitious shoot buds produced multiple shoots on MS medium supplemented with BAP (0.4 mg/l). Well-developed germinated plantlets were acclimatized in the screen house with 90 % survivability. Histology studies confirmed that the regeneration of D. rotundata reported here followed dual regeneration pathways. The embryogenic calli regenerated through development of somatic embryos and germinated into complete plantlets, however non-embryogenic calli regenerated through organogenesis and developed multiple shoots. The developed protocol has potential for somatic hybridization, mass clonal propagation, and genetic transformation applications.

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Section: Introductionmentioning

confidence: 99%

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Rajesh

Tripathi

2016

Plant Cell Tiss Organ Cult

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“…In contrast, the scores of S. bradys damage are observed with significant probability (P<0.001) ranging from 1 to 3 revealing significant difference between studied clones. (Adegbite et al 2006). This information can help to understand the tolerance of some clones of D. rotundata to both studied nematodes contrasting with data recorded for D. alata : They are probably more adapted to West Africa ecology constraints than D. alata .…”

Section: Climatic Growth Conditionsmentioning

confidence: 87%

Screening of new yam clones (<i>D. alata</i> and <i>D. rotundata</i>) in nematode prone ecology of guinea savanna zone in West Africa.

Ettien¹,

Fatogoma²,

Brahima³

2013

J. App. Bioscience.

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Objectives: The main objective of this study was to identify some yam assessions that are tolerant to the most invasive nematodes like Scutellonema bradys and Meloidogyne spp. Methodology and results: New assessions of Dioscorea spp. were screened in the savanna zone of central Côte d'Ivoire. Fourteen of D. alata and thirty seven assessions of D. rotundata were tested respectively and separately against two and four local yam varieties in an augmented design from 2000 to 2001. Nine varieties of D. rotundata were more resistant to S. bradys and Meloidogyne when D. alata were less tolerant. The effect of S. bradys on D. alata was higher in 2001 compared to 2000. The effect of Meloidogyne was significantly (P<0.001) depressive on the production of D. rotundata every year while they were tolerant to S. bradys. Conclusion and application: Identified assessions among D. rotundata can be considered as promising varieties in nematode prone ecology. These varieties are 95/01864,

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“…R. reniformis is usually found in mixed populations with M. incognita and the disease symptoms in such situation is often only associated with root-knot nematodes. Many authors in Nigeria have found the reniform nematode in moderate populations associated with various crops (Adegbite et al, 2006;Olabiyi et al, 2009;Afolami et al, 2014;Daramola et al, 2015) but they did not consider the nematode to be damaging. This implies that the reniform nematode can be present in damaging populations and be left undiagnosed and uncontrolled due to lack of association as a potential pest problem.…”

Section: Discussionmentioning

confidence: 99%

“…Yield increases on okra, tomato, and lettuce of 19, 15, and 57% were obtained with treatment of granular nematicides, respectively on Rotylenchulus reniformis infested soil (Sikora and Fernandez, 2005). R. reniformis has been reported to be associated with various crops (Adegbite et al, 2006 ;Daramola and Afolami, 2014 ;Olabiyi et al, 2009) in Nigeria but has not been clearly connected to any quantitative loss of yield in Nigeria. The aim of this study was to assess the effect of the reniform and root-knot nematodes associated with the infection of okra.…”

Section: Introductionmentioning

confidence: 99%

Comparative Effect of Rotylenchulus reniformis and Meloidogyne incognita on the Productivity of okra in Nigeria

2018

Aust. J. Basic & Appl. Sci.

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Okra has risen in importance from being the fifth popularly produced vegetable to the second in the last few years in Nigeria. This study therefore evaluated the impact of growing okra in soils infested with Rotylenchulus reniformis and Meloidogyne incognita. A pot experiment was conducted in the screenhouse with three popular okra cultivars (NH 47-4, LD66-1 and Clemson spineless) inoculated with either R. reniformis or M. incognita and compared to uninoculated plants. The experiment was arranged in a completely randomized design with five replicates. The field trial was conducted using one cultivar, Clemson spineless and the same treatments as the pot trial laid out in a randomised complete block design with four replications. Data were collected on number of leaves, leaf area, plant height, number of fruits and fruit weight and submitted for analysis of variance. Significant reduction in number of leaves and plant height was observed in inoculated compared to uninoculated plants. Inoculated plant produced smaller and fewer fruits in comparison to the control. Yield reduction in pots was 31-72% in pot experiments with R. reniformis and 51-76% in pots with M. incognita. Yield of Clemson spineless in the field was reduced by 36% and 40% by M. incognita and R. reniformis respectively. The reniform nematode has gone beyond being a potential pest of vegetable crops such as okra in Nigeria to being an actual pest that requires serious management interventions.Citation: Claudius-Cole A.O. Comparative Effect of Rotylenchulus reniformis and Meloidogyne incognita on the Productivity of okra in Nigeria. Australian Journal of Basic and Applied Sciences, 12(9): 20-25.

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Survey of Plant-Parasitic Nematodes Associated with Yams in Ogun and Osun States of Nigeria

Cited by 19 publications

References 5 publications

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Plant regeneration from axillary bud derived callus in white yam (Dioscorea rotundata)

Screening of new yam clones (<i>D. alata</i> and <i>D. rotundata</i>) in nematode prone ecology of guinea savanna zone in West Africa.

Comparative Effect of Rotylenchulus reniformis and Meloidogyne incognita on the Productivity of okra in Nigeria

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