Survey of mycobiota, black Aspergillus and ochratoxin A occurrence on Brazilian wine grapes

Einloft, Tiago Centeno; Hoeltz, Michele; Teixeira, Tádzio Ribeiro; Oldoni, Verônica Petri; Manfroi, Vítor; Noll, Isa Beatriz

doi:10.1007/s13213-016-1236-0

Cited by 6 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Specifically, certain fungi may be recognized as endophytes and bio-control agents, providing a competitive advantage over biotic and abiotic stresses 6 . Phytopathogenic fungi are able to cause plant disease or lead to fruit post-harvest deterioration, significantly affecting fruit yield, quality and marketing value 7 , 8 . In addition, fungi can produce toxic secondary metabolites, namely, mycotoxins 9 .…”

Section: Introductionmentioning

confidence: 99%

Differentiated surface fungal communities at point of harvest on apple fruits from rural and peri-urban orchards

Shen

Nie

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The diverse fungal communities that colonize fruit surfaces are closely associated with fruit development, preservation and quality control. However, the overall fungi adhering to the fruit surface and the inference of environmental factors are still unknown. Here, we characterized the fungal signatures on apple surfaces by sequencing internal transcribed spacer 1 (ITS1) region. We collected the surface fungal communities from apple fruits cultivated in rural and peri-urban orchards. A total of 111 fungal genera belonging to 4 phyla were identified, showing remarkable fungal diversity on the apple surface. Comparative analysis of rural samples harboured higher fungal diversity than those from peri-urban orchards. In addition, fungal composition varied significantly across apple samples. At the genus level, the protective genera Coniothyrium, Paraphaeosphaeria and Periconia were enriched in rural samples. The pathogenic genera Acremonium, Aspergillus, Penicillium and Tilletiposis were enriched in peri-urban samples. Our findings indicate that rural samples maintained more diverse fungal communities on apple surfaces, whereas peri-urban-planted apple carried potential pathogenic risks. This study sheds light on ways to improve fruit cultivation and disease prevention practices.

show abstract

Section: Introductionmentioning

confidence: 99%

Differentiated surface fungal communities at point of harvest on apple fruits from rural and peri-urban orchards

Shen

Nie

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Viticulture is an important activity in many countries (Einloft et al, 2017). Vine growing and viticulture have a very long tradition in Slovakia and are parts of the country's cultural and historical heritage.…”

Section: Introductionmentioning

confidence: 99%

Survey of mycobiota on Slovakian wine grapes from Small Carpathians wine region

Felšöciová

Kačániová

František

2020

Potr. S. J. F. Sci.

View full text Add to dashboard Cite

A total of 13 samples of grapes (bunches) without apparent fungal contamination were analyzed. The samples were collected during the 2019 harvest from Vrbové village in the Small Carpathian region of Slovakia. For the isolation of fungi were used the direct plating technique on DRBC plates. The plates were incubated aerobically at 25 ±1 °C for one week in the dark. The data obtained from the cultivation of the grape berry samples revealed a high diversity of fungal species (a total of 1044 isolates were obtained). Alternaria and Rhizopus were the main components of the wine grape mycobiota of the Vrbovský subregion at harvest time (92%, each), followed by Cladosporium (85%), Penicillium (77%), Botrytis and Epicoccum (54%, each). The most abundant genera found by descending order were Penicillium (25%), Alternaria (24%), Cladosporium (20%), and Rhizopus (12%) and only in minor percentage by Aspergillus (3%) among others. The main fungal species isolated from genera Penicillium and Aspergillus were Penicillium expansum (57% RD) and A. section Nigri (97% RD). Of 17 analyzed Penicillium strains, 65% were able to produce at least one of the six mycotoxins analyzed in in vitro conditions by means of thin-layer chromatography method: citrinin, griseofulvin, patulin, cyclopiazonic acid, penitrem A, and roquefortin C.

show abstract

“…32 In addition to A. niger being a good competitor, the microorganism adapts extremely well to the environment present in vineyards and grapes. 33 Thus, the highest OTA production by this species in the grape varieties tested may be related to a better substrate adaptation. However, the best growth conditions are not always related to the higher production of secondary metabolites.…”

Section: ■ Results and Discussionmentioning

confidence: 92%

“…Although most studies consider A. carbonarius the main OTA-producing species at higher levels, some strains of A. niger may be higher producers than A. cabonarius. In addition to A. niger being a good competitor, the microorganism adapts extremely well to the environment present in vineyards and grapes . Thus, the highest OTA production by this species in the grape varieties tested may be related to a better substrate adaptation.…”

Section: Resultsmentioning

confidence: 98%

Influence of Maturation Stages in Different Varieties of Wine Grapes (Vitis vinifera) on the Production of Ochratoxin A and Its Modified Forms by Aspergillus carbonarius and Aspergillus niger

Freire

Guerreiro

Caramês

et al. 2018

J. Agric. Food Chem.

View full text Add to dashboard Cite

Ochratoxin A is the main contaminant mycotoxin of grapes produced mainly by Aspergillus niger and Aspergillus carbonarius. Besides, it is possible that the formation of modified mycotoxin occurs through the plant defense mechanism or also by fungus actions itself. The objective of this study was to evaluate the influence of grape variety and maturation stage on the formation of OTA and modified mycotoxin. The determination of OTA was performed by high-performance liquid chromatography, and a high-resolution mass spectrometry was used for the detection of modified ochratoxin. A positive correlation was observed between the following grapes physicochemical parameters: pH, total soluble solids, total glycosides in glucose, total anthocyanin, and OTA levels produced by A. niger and A. carbonarius. Therefore, the higher the concentrations of these parameters, the greater the production of mycotoxin in grapes. Among the elected targets, we identified the 14-decarboxy-ochratoxin A in Muscat Italia variety at veraison and 15 days after the beginning of veraison stages; and ethylamide-ochratoxin A as a biomarker in the Syrah variety at the ripeness stage.

show abstract

Survey of mycobiota, black Aspergillus and ochratoxin A occurrence on Brazilian wine grapes

Cited by 6 publications

References 39 publications

Differentiated surface fungal communities at point of harvest on apple fruits from rural and peri-urban orchards

Differentiated surface fungal communities at point of harvest on apple fruits from rural and peri-urban orchards

Survey of mycobiota on Slovakian wine grapes from Small Carpathians wine region

Influence of Maturation Stages in Different Varieties of Wine Grapes (Vitis vinifera) on the Production of Ochratoxin A and Its Modified Forms by Aspergillus carbonarius and Aspergillus niger

Contact Info

Product

Resources

About