Survey of Klebsiella pneumoniae producing extended-spectrum beta-lactamases: prevalence of TEM-3 and first identification of TEM-26 in France

Abstract

Crude extracts from 115 extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates were analyzed biochemically. The TEM-3 type was encountered 108 times, SHV types were encountered 7 times, and the TEM-26 type was encountered only once. For the last one, the gene was identified; an adenine was detected at position 925, as in blaTEM-26B not in blaTEM-26.

Evolution and epidemiology of extended-spectrum β-lactamases (ESBLs) and ESBL-producing microorganisms

“…The concept of multi‐ β ‐lactam pressure does not explain all the phenomena that are observed in ESBL evolution and epidemiology. ESBLs characterised by highly selective substrate preferences are being selected [50,68,80] and may remain prevalent at the level of a single medical center or on a wider geographic scale [78,85,86]. Moreover, as Blázquez et al.…”

“…An ESBL variant (ESBL producer strain) may appear in a center due to de novo selection, which may result in a novel type of enzyme [17,57,60,89,130] or in one that has been previously identified in another institution (convergent evolution) [67][68][69]137]. It may also be imported by a patient from another center, even though that may be located in another city or country [31,57,[138][139][140][141].…”

“…For example, plasmid transfer was revealed to be responsible for the spread of TEM-3 in France, identified in strains of 10 different species in 26 hospitals by 1989 [155,156], and of CTX-M-3 in Poland, found in eight species in 15 hospitals by 2000 [86]. Finally, convergent evolution may also lead to a wide geographic representation of an ESBL variant [67][68][69][70], and this has probably been a major factor in the 'international career' of SHV-5, despite documented cases of its importation to one country from another [138]. SHV-5 has been found in almost every country in which ESBLs have ever been studied down to the molecular level [57,64,95,97,134,148,[157][158][159][160][161][162][163][164][165], and in some of these, e.g.…”

“…The concept of multi-b-lactam pressure does not explain all the phenomena that are observed in ESBL evolution and epidemiology. ESBLs characterised by highly selective substrate preferences are being selected [50,68,80] and may remain prevalent at the level of a single medical center or on a wider ß 2001 Copyright by the European Society of Clinical Microbiology and Infectious Diseases, CMI, 7, 597-608 geographic scale [78,85,86]. Moreover, as Blázquez et al point out, enzyme variants with 'rare' spectrum-extending mutations may also be sporadically selected.…”

Dissemination and persistence of a plasmid-mediated TEM-3-like β-lactamase, TEM-139, among Enterobacteriaceae in Bulgaria

“…Since then, TEM-3-producing Enterobacteriaceae have been reported from France throughout the last two decades [2][3][4]. They have also been found in other countries, e.g.…”