Survey of human and rat microsatellites

Beckmann, Jacques S.; Weber, James L.

doi:10.1016/0888-7543(92)90285-z

Cited by 473 publications

(357 citation statements)

References 15 publications

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“…Might it spread by retrotransposition? Various retrotransposable elements have been suspected of spreading microsatellites throughout genomes [62][63][64]. However, the lack of homology of TcREP-class repeats to any known retrotransposable element and the failure to form a consistent RNA secondary structure do not support this view.…”

Section: Discussionmentioning

confidence: 99%

Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Johnson

Webster

Adam

et al. 2006

Molecular and Biochemical Parasitology

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Section: Discussionmentioning

confidence: 99%

Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Johnson

Webster

Adam

et al. 2006

Molecular and Biochemical Parasitology

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“…Microsatellite sequences of 1-5 nucleotides per unit repeat are found throughout the human genome [1][2][3][4][5][6][7][8]. Microsatellite sequences are often hotspots for mutation, and an increase in mutagenesis is observed as the length of the repeated sequence increases [9,10].…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles

Jacob

Eckert

2007

Mutation Research/Fundamental and Molecular Mechanisms of Mutag

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Slipped strand mispairing during DNA synthesis is one proposed mechanism for microsatellite or short tandem repeat (STR) mutation. However, the DNA polymerase(s) responsible for STR mutagenesis have not been determined. In this study, we investigated the effect of the Escherichia coli dinB gene product (Pol IV) on mononucleotide and dinucleotide repeat stability, using an HSVtk gene episomal reporter system for microsatellite mutations. For the control vector (HSV-tk gene only) we observed a statistically significant 3.5-fold lower median mutation frequency in dinB -than dinB + cells (p<0.001, Wilcoxon Mann Whitney Test). For vectors containing an in-frame mononucleotide allele ([G/C] 10 ) or either of two dinucleotide alleles ([GT/CA] 10 and [TC/AG] 11 ) we observed no statistically significant difference in the overall HSV-tk mutation frequency observed between dinB + and dinB -strains. To determine if a mutational bias exists for mutations made by Pol IV, mutational spectra were generated for each STR vector and strain. No statistically significant differences between strains were observed for either the proportion of mutational events at the STR or STR specificity among the three vectors. However, the specificity of mutational events at the STR alleles in each strain varied in a statistically significant manner as a consequence of microsatellite sequence. Our results indicate that while Pol IV contributes to spontaneous mutations within the HSV-tk coding sequence, Pol IV does not play a significant role in spontaneous mutagenesis at [G/ C] 10 , [GT/CA] 10 , or [TC/AG] 11 microsatellite alleles. Our data demonstrate that in a wild type genetic background, the major factor influencing microsatellite mutagenesis is the allelic sequence composition.

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“…The most abundant microsatellite in several wellknown mammals is (AC) n (Beckmann and Weber, 1992), while in many plant species they are (AT) n or (AG) n (Wang et al, 1994). A high abundance of (GA) n microsatellites compared to other dinucleotid SSR has been observed in plant genomes such as Oryza, Aegilops, Arabidopsis or Brassica (Gupta and Varshney, 2000;Guyomarc et al, 2002;Suwabe et al, 2002;Uzunova and Ecke, 1999).…”

Section: Introductionmentioning

confidence: 99%

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

Yaish

Vega

2003

Genet. Mol. Biol.

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The isolation of (GA) n microsatellites using a highly microsatellite-enriched library is described for the first time in common bean (Phaseolus vulgaris L.). A relatively simple and effective method to isolate DNA repeats from microsatellite-enriched libraries based on hybridization-capture of repeat regions using biotin-conjugated oligonucleotids and non-radioactive colony hybridization was carried out. PCR products from 200 to 800 bp were obtained and cloned. Of the 60 clones sequenced, 21 yielded (GA) n microsatellites with n values equal or higher than six. These (GA) n microsatellite-containing loci could be useful for further genetic mapping studies. A (GA) n microsatellite linked to a putative MADS-box gene was identified. This sequence, which represents the first MADS-box locus described to date in common bean, showed a very high similarity with other known MADS-box sequences and was grouped within the AGL2 subfamily cluster of the Arabidopsis MADS-box genes. The vicinity of microsatellites to some genes is also discussed.

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Survey of human and rat microsatellites

Cited by 473 publications

References 15 publications

Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles

Isolation of (GA)n microsatellite sequences and description of a predicted MADS-box sequence isolated from common bean (Phaseolus vulgaris L.)

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