Survey of forkhead domain encoding genes in the <i>Drosophila</i> genome: Classification and embryonic expression patterns

Lee, Hsiu‐Hsiang; Frasch, Manfred

doi:10.1002/dvdy.10443

Cited by 75 publications

(87 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FoxJ, FoxL, and FoxQ and FoxN (sensu lato FoxL1+FoxL2, FoxQ1+FoxQ2, Fox1/4 +Fox2/3) were not monophyletic assemblages. Lee and Frasch (Lee & Frasch, 2004) failed to find an orthologous Drosophila gene in FoxJ. Yet our analyses found their orphan taxon DM CG 12632 sister to Ae.…”

Section: The Fox Genementioning

confidence: 64%

“…Neighbor-joining (NJ) is a methodology for the construction of phylogenetic hypotheses that should be used only to produce a starting tree upon which more thorough searches can be performed (Swofford et al, 1996). Nevertheless, our more rigorous analyses share some features in common with those of past (Lee & Frasch, 2004;Mazet et al, 2003).…”

Section: Discussionmentioning

confidence: 84%

“…Similar searches resulted in the identification of 18 loci from the genome of the malaria mosquito An. gambiae (Mazet et al, 2003), and 16/17 loci from the genome of the fruit fly D. melanogaster (Lee & Frasch, 2004;Mazet et al, 2003). Of possible significance, the number of Fox factors predicted to occur in these three dipteran insects is less than half of that proposed for humans (40 Fox loci for humans are listed at the following web site: http://www.biology.pomona.edu/Fox.html) (Kaestner et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Forkhead transcription factors regulate mosquito reproduction

Hansen

Sieglaff

Munro

et al. 2007

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Forkhead box (Fox) genes encode a family of transcription factors defined by a 'winged helix' DNAbinding domain. In this study we aimed to identify Fox factors that are expressed within the fat body of the yellow fever mosquito Aedes aegypti, and determine whether any of these are involved in the regulation of mosquito yolk protein gene expression. The Ae. aegypti genome contains eighteen loci that encode putative Fox factors. Our stringent cladistic analysis has profound implications for the use of Fox genes as phylogenetic markers. Twelve Ae. aegypti Fox genes are expressed within various tissues of adult females, six of which are expressed within the fat body. All six Fox genes expressed in the fat body displayed dynamic expression profiles following a blood meal. We knocked down the 'fat body Foxes' through RNAi to determine whether these "knockdowns" hindered amino acidinduced vitellogenin gene expression. We also determined the effect of these knockdowns on the number of eggs deposited following a blood meal. Knockdown of FoxN1, FoxN2, FoxL, and FoxO, had a negative effect on amino acid-induced vitellogenin gene expression and resulted in significantly fewer eggs laid. Our analysis stresses the importance of Fox transcription factors in regulating mosquito reproduction.

show abstract

Section: The Fox Genementioning

confidence: 64%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Forkhead transcription factors regulate mosquito reproduction

Hansen

Sieglaff

Munro

et al. 2007

Insect Biochemistry and Molecular Biology

View full text Add to dashboard Cite

show abstract

“…2 O-V). In three cases (bowl, CG9571, and D͞fsh), the newly identified CRMs direct patterns similar to their associated endogenous genes (29)(30)(31)(32). The final CRM (bl͞Mir7) is located in the sixth intron of the bl gene and directs a strong anterior domain of expression (Fig.…”

Section: Resultsmentioning

confidence: 91%

The role of binding site cluster strength in Bicoid-dependent patterning in Drosophila

Ochoa-Espinosa

Yücel

Kaplan

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

166

169

View full text Add to dashboard Cite

The maternal morphogen Bicoid (Bcd) is distributed in an embryonic gradient that is critical for patterning the anterior-posterior (AP) body plan in Drosophila. Previous work identified several target genes that respond directly to Bcd-dependent activation. Positioning of these targets along the AP axis is thought to be controlled by cis-regulatory modules (CRMs) that contain clusters of Bcd-binding sites of different ''strengths.'' Here we use a combination of Bcd-site cluster analysis and evolutionary conservation to predict Bcd-dependent CRMs. We tested 14 predicted CRMs by in vivo reporter gene assays; 11 show Bcd-dependent activation, which brings the total number of known Bcd target elements to 21. Some CRMs drive expression patterns that are restricted to the most anterior part of the embryo, whereas others extend into middle and posterior regions. However, we do not detect a strong correlation between AP position of target gene expression and the strength of Bcd site clusters alone. Rather, we find that binding sites for other activators, including Hunchback and Caudal correlate with CRM expression in middle and posterior body regions. Also, many Bcd-dependent CRMs contain clusters of sites for the gap protein Kruppel, which may limit the posterior extent of activation by the Bcd gradient. We propose that the key design principle in AP patterning is the differential integration of positive and negative transcriptional information at the level of individual CRMs for each target gene.morphogen ͉ network ͉ transcription G radients of two transcription factors, Bicoid (Bcd) and Dorsal (Dl), are critical for patterning the major body plan axes in the Drosophila embryo. This studied focused on Bcd, a maternal effect gene whose mRNA is initially localized at the anterior pole of the oocyte (Fig. 1A) (1). Upon egg deposition, the trapped bcd RNA is translated, and a nuclear gradient forms, with highest concentrations near the anterior pole and progressively lower concentrations in more posterior regions (Fig. 1B) (2). Bcd protein distribution matches its biological activity, with high levels required for the positioning of the most anterior structures, intermediate levels for head structures, and low levels for the thoracic and anterior abdominal segments. Varying the shape of the Bcd gradient has a direct effect on positional identity in the blastoderm (3). Increasing the maternal input of bcd can shift morphological landmarks posteriorly, whereas decreasing it shifts them anteriorly. These experiments suggest that the concentration of Bcd protein present at each position along the length of the embryo (EL) determines the destiny of cells that will occupy that region.Bcd protein contains a homeodomain and functions as a morphogen by activating the transcription of multiple target genes in different positions along the anterior-posterior (AP) axis (reviewed in refs. 4 and 5). Driever, Thoma, and Nüsslein-Volhard (6) first proposed that differential positioning could occur by means of gene-specific regulatory ele...

show abstract

“…All forkhead family members contain the ~100 amino acid, monomeric DNA "wingedhelix" binding domain of the founding members: Drosophila forkhead [Lee and Frasch, 2004] and hepatocyte nuclear factor 3a [Tao and Lai, 1992]. Forkhead gene products play pivotal roles in organogenesis, including patterning and morphogenesis, through the regulation of the proliferation and cell fate specification [Solomon et al, 2003b].…”

Section: Introductionmentioning

confidence: 99%

A missense mutation within the fork-head domain of the forkhead box G1 Gene (FOXG1) affects its nuclear localization

et al. 2010

View full text Add to dashboard Cite

ABSTRACT:The forkhead box G1 (FOXG1) gene has recently been associated with the congenital variant of Rett syndrome, and so far 17 mutations have been reported. We screened the coding region in 150 patients affected by postnatal microcephaly, and identified two mutations: the c.326C>T (p.P109L) substitution inherited from the healthy father; and the de novo c.730C>T transition, which induces the p.R244C mutation within the DNA-binding forkhead domain. This latter mutation is carried by an 8-year-old girl, who presented a phenotype reminiscent of the congenital variant of Rett syndrome. Immunofluorescence analysis of the wild-type protein revealed a homogeneous nuclear staining excluding the nucleoli, while the p.R244C mutant showed abnormal nuclear foci in a large proportion of cells, suggesting that its mislocalization may reduce and/or impair target recognition. Interestingly, this missense mutation results in a mislocalization of FoxG1 to specific nuclear foci referred to as nuclear speckles, and affects the cyclin-dependent kinase inhibitor p21 CDKN1A expression. Because CDKL5, which is involved in the early-onset variant of Rett syndrome, is also located in these speckles, we suggest that disregulation of the dynamic behaviour of nuclear speckles may functionally link these two proteins, which are both involved in atypical forms of Rett syndrome.

show abstract

Survey of forkhead domain encoding genes in the Drosophila genome: Classification and embryonic expression patterns

Cited by 75 publications

References 58 publications

Forkhead transcription factors regulate mosquito reproduction

Forkhead transcription factors regulate mosquito reproduction

The role of binding site cluster strength in Bicoid-dependent patterning in Drosophila

A missense mutation within the fork-head domain of the forkhead box G1 Gene (FOXG1) affects its nuclear localization

Contact Info

Product

Resources

About