Survey of dentin sialophosphoprotein and its cognate matrix metalloproteinase‐20 in human cancers

Abstract

Background Matrix metalloproteinases‐20 (MMP20) expression is widely regarded as tooth specific, with expression limited to dental hard tissues. Recently, we reported MMP20 expression and interaction with dentin sialophosphoprotein (DSPP), a member of the Small Integrin Binding Ligand N‐linked Glycoproteins (SIBLINGs), in human oral squamous cell carcinoma (OSCC) and dysplastic oral premalignant lesions (OPLs), suggesting a role for MMP20‐DSPP interaction in oral carcinogenesis. Methods This study aimed to survey the expression of MMP20 and its cognate DSPP partner in the breast, colon, prostate, thyroid, and cervical neoplasms. Using commercially available tissue microarrays (TMAs) and cell lines, we performed immunohistochemistry, immunofluorescence, proximity ligation assay, and western blot experiments to determine the expressions of MMP20 and DSPP in the breast, colon, prostate, thyroid, cervical neoplasms, and their normal counterparts. Results Significantly high expression levels of MMP20 and DSPP were observed in the malignant breast, colon, prostate, thyroid, and cervical neoplasms compared with their benign and normal counterparts. Furthermore, MMP20 levels increased with advanced stages of colon and thyroid cancers. DSPP expression increased significantly with tumor stage in all cancers examined. Conclusions The co‐localization and potential MMP20‐DSPP interaction previously reported in oral cancers are present in other cancers. These results suggest MMP20‐DSPP pairing as a potential marker of disease activity in some epithelial cancers with diagnostic and prognostic implications.

The prevalence of germline pathogenic variants in Estonian colorectal cancer patients: results from routine clinical setting 2016–2021

“…First, a 64-year-old man with colorectal polyposis and dentinogenesis imperfecta with a finding of NM_014208.3 (DSPP):c.52G>T (p.Val18Phe). DSPP gene has been previously linked to oral cancers ( Joshi et al, 2010 ), but MMP20-DSPP co-localization and interaction has been observed in breast, colorectal and other cancers as well ( Aseervatham et al, 2019 ). TSO/TSOE was also used to diagnose a 72-year-old woman who had had ascending colon adenocarcinoma at the age of 64 and with a family history of muscle disease; a known familial CHEK2 variant NM_007194.3(CHEK2):c.319+2T>A p.?…”

Development and validation of a novel immune-related prognostic signature in lung squamous cell carcinoma patients

“…Aseervatham et al reported that Matrix Metalloproteinase 20 (MMP20) played a tumorigenic role on Oral Squamous Cell Carcinomas (OSCCs) by upregulating the genes related to invasion, metastasis, angiogenesis, and epithelial-mesenchymal transition (EMT) 24 . They also elucidated that MMP20 and its cognate DSPP paring a potential marker of some epithelial cancers 25 . The expression of C18orf26 induced the activation of Akt and sustaining the high proliferation of tumor cells, making it a potential novel therapeutic target 26 .…”

Development and validation of a novel immune-related prognostic signature in lung squamous cell carcinoma patients

“…20 They also elucidated that MMP20 and its cognate DSPP paring a potential marker of some epithelial cancers. 21 The expression of C18orf26 induced the activation of Akt and sustaining the high proliferation of tumor cells, making it a potential novel therapeutic target. 22 Handa et al reported that the high CASP14 expression is associated with proliferation, cancer stemness of breast cancer.…”