Survey of bovine mycotic mastitis in dairy herds in the State of S�o Paulo, Brazil

Abstract

The purpose of this study was to isolate fungi from the quarter milk of cow udders from several dairy herds and to identify the different genera and species involved in mastitis. A total of 2078 milk samples from normal, clinical and subclinical mastitis quarters from 22 dairy herds of 16 districts in the State of São Paulo, Brazil was utilized in this survey. Two hundred and fifty one (12.07%) fungi were isolated from the samples. Two hundred and eight of these (82.86%) were yeasts and 30 (11.95%) were moulds. The fungi were isolated in pure culture (24.77%) or in cultures mixed with bacteria (72.22%). The yeasts isolated were: Cryptococcus spp. (71 strains), Rhodotorula spp. (40), Candida spp. (68), Trichosporon cutaneum (21), Aureobasidium pullulans (7), and Pichia ohmeri (1). Moulds classified in following genera were also isolated: Aspergillus (3), Penicillium (3), Alternaria (3), Phoma (3), Epicoccum (2), and Geotrichum (16).

Molecular-Based Identification of Yeasts Isolated from Bovine Clinical Mastitis in Japan

“…Environmental contamination resulting from poor hygiene during the milking process and poor equipment cleaning may lead to the development of mastitis. In different countries, the frequency of yeast isolation varies considerably, with reported rates of 1.3% in Denmark [1], 9.6% in Poland [7] and 12.1% in Brazil [3]. In the present study, yeasts were isolated from 1.8% of total clinical samples.…”

Fatores de risco associados à mastite bovina causada por Prototheca zopfii

“…Além disso, como o diagnóstico pode apresentar dificuldades na identificação das algas do gênero Prototheca, freqüentemente, vacas acometidas por infecções intramamárias subclínicas com resultado microbiológico negativo não são medicadas e podem disseminar o agente para outros animais do rebanho, principalmente durante a ordenha (COSTA et al, 1996b). COSTA et al (1993) observaram que animais com mastite crônica, submetidos à terapia antibacteriana excessiva e repetida, são mais suscetíveis às infecções fúngicas ou mistas. Neste estudo, todas as vacas com mastite por P. zopfii foram submetidas a terapias anteriores.…”

Yeasts as a causative agent of bovine mastitis in Greece

“…In European countries this percentage varies from 4.4% up to 6% (Hofmann et al 1965, Chermette and Bussieras 1993, Lagneau et al 1996, Moretti et al 1998, in the USA up to 7% (Richard et al 1980, Kirk andBartllet 1986), while in other countries it is higher than 12% (Awad et al 1980, Kitamura et al 1990, Singh et al 1992, Costa, et al 1993). …”