Survey for the presence of BK, JC, KI, WU and Merkel cell polyomaviruses in human brain tissues

Lam, Wai Yip; Leung, Bo Wah; Chu, Ida Miu-Ting; Chan, Alexander C.L.; Ng, Ho‐Keung; Chan, Paul K.S.

doi:10.1016/j.jcv.2010.01.017

Cited by 13 publications

(8 citation statements)

References 36 publications

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“…This differed from the study by Barzon et al [8] where 14.3% of the samples from PML patients had detectable KI and WU PyV, but is consistent with the findings of Focosi et al [9] and Maggi et al [15], who also reported no KIPyV and WUPyV DNA in PML and other immunosuppressed patients. Moreover, our results are also supported by the findings by Lam et al [16], who did not detect KIPyV, WUPyV and MCPyV in 300 brain samples from 30 individuals (Table S2). Lastly, our study is the first to include results from two different laboratories using different PCR methods, thereby confirming the study findings.…”

Section: Discussionsupporting

confidence: 91%

Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy

et al. 2011

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Conflicting prevalence of newly identified KI(KIPyV), WU(WUPyV) and Merkel Cell Carcinoma(MCPyV) polyomaviruses have been reported in progressive multifocal leukoencephalopathy(PML) patient samples, ranging from 0 to 14.3%. We analyzed the prevalence of these polyomaviruses in cerebrospinal fluid(CSF), peripheral blood mononuclear cells(PBMC), and bone marrow samples from PML patients, immunosuppressed individuals with or without HIV, and multiple sclerosis(MS) patients. Distinct PCR tests for KIPyV, WUPyV and MCPyV DNA performed in two independent laboratories detected low levels of MCPyV DNA only in 1/269 samples. The infrequent detections of these viruses in multiple samples from immunosuppressed individuals including those with PML suggest that their reactivation mechanisms may be different from that of JC polyomavirus (JCPyV) and that they do not play a role in the pathogenesis of PML.

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Section: Discussionsupporting

confidence: 91%

Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy

et al. 2011

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“…It should be noted that the current study included no neuroblastoma patients and only two cases of childhood CNS tumor (one medulloblastoma and one xanthoastrocytoma), both of which were negative for the MCPyV LT‐Ag sequence. Although recent work on autopsy brain tissues collected from 30 individuals who died from diseases unrelated to CNS showed negative results for MCPyV sequences by nested PCR assay [Lam et al, ], this finding does not exclude the possible role of MCPyV in CNS tumor formation.…”

Section: Discussionmentioning

confidence: 92%

Detection of Merkel cell polyomavirus large T-antigen sequences in human central nervous system tumors

et al. 2015

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Despite decades of epidemiological investigation, little is known about the etiology of the central nervous system (CNS) tumors, and few well-established risk factors have been recognized. This study tested the presence of Merkel cell polyomavirus (MCPyV), the only member of the Polyomaviridae family convincingly linked to human cancer, in diverse CNS malignancies. In total, 58 CNS tumor biopsies were analyzed for the MCPyV large T-antigen (LT-Ag) gene by quantitative real-time PCR. Merkel cell polyomavirus LT-Ag DNA load was determined as viral copies per cell and viral copies per microliter of purified genomic DNA from CNS tumor samples. The MCPyV LT-Ag sequence was detected in 34 (58.6%) of the 58 tested samples. Viral LT-Ag was quantified in 19.0% of schwannomas, 13.8% of meningiomas, and 5.2% of pituitary adenomas. The difference between MCPyV positivity in different types of CNS malignancies was not statistically significant (P = 0.066). The mean LT-Ag copy number in 34 positive samples was 744.5 ± 737.7 and 0.056 × 10(-3) ± 0.091 × 10(-3) per microliter and per cell, respectively. Among MCPyV-positive CNS tumors, the mean MCPyV copy number was higher in meningiomas (993.8 ± 853.2 copy per microliter and 0.098 × 10(-3) ± 0.108 × 10(-3) copy per cell). Multiple linear regression analysis revealed statistically significant difference in MCPyV copy number between meningioma and other CNS tumor types, when the model was adjusted for age and sex (P = 0.024). This study shows the first evidence of the detection of MCPyV LT-Ag sequence at a low copy number in human CNS tumors.

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“…As mentioned earlier, real-time PCR results for MCPyV were all negative except in one case, a liver transplant recipient. Data are conflicting regarding the detection of MCPyV DNA in different types of brain tumours and its detection in cases with CNS infections has not been reported [34,35,36].…”

Section: Discussionmentioning

confidence: 99%

Viral Sequences Detection by High-Throughput Sequencing in Cerebrospinal Fluid of Individuals with and without Central Nervous System Disease

Schibler

Brito

Zanella

et al. 2019

Genes

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: Meningitis, encephalitis, and myelitis are various forms of acute central nervous system (CNS) inflammation, which can coexist and lead to serious sequelae. Known aetiologies include infections and immune-mediated processes. Despite advances in clinical microbiology over the past decades, the cause of acute CNS inflammation remains unknown in approximately 50% of cases. High-throughput sequencing was performed to search for viral sequences in cerebrospinal fluid (CSF) samples collected from 26 patients considered to have acute CNS inflammation of unknown origin, and 10 patients with defined causes of CNS diseases. In order to better grasp the clinical significance of viral sequence data obtained in CSF, 30 patients without CNS disease who had a lumbar puncture performed during elective spinal anaesthesia were also analysed. One case of human astrovirus (HAstV)-MLB2-related meningitis and disseminated infection was identified. No other viral sequences that can easily be linked to CNS inflammation were detected. Viral sequences obtained in all patient groups are discussed. While some of them reflect harmless viral infections, others result from reagent or sample contamination, as well as index hopping. Altogether, this study highlights the potential of high-throughput sequencing in identifying previously unknown viral neuropathogens, as well as the interpretation issues related to its application in clinical microbiology.

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Survey for the presence of BK, JC, KI, WU and Merkel cell polyomaviruses in human brain tissues

Cited by 13 publications

References 36 publications

Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy

Infrequent Detection of KI, WU and MC Polyomaviruses in Immunosuppressed Individuals with or without Progressive Multifocal Leukoencephalopathy

Detection of Merkel cell polyomavirus large T-antigen sequences in human central nervous system tumors

Viral Sequences Detection by High-Throughput Sequencing in Cerebrospinal Fluid of Individuals with and without Central Nervous System Disease

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