Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing

Rosenberg, Julian N.; Cady, Nathaniel C.

doi:10.1016/j.copbio.2021.07.004

Cited by 8 publications

(6 citation statements)

References 51 publications

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…In the log or exponential phase, the cells increased in both number and size. The plateau was an increase in cells that have experienced confluent for an attachment site to occur or when there are interactions between cells and the cultural environment 19 Cell viability was determined using the trypan blue staining method, which was the percentage ratio of the number of viable cells (viable) indicated by fluorescent and dead cells (non-viable). They were characterized by dark blue cells because the dead ones lose the ability to maintain the integrity and permeability of the plasma membrane for trypan blue to be absorbed.…”

Section: Vero Cell Linementioning

confidence: 99%

“…Furthermore, all samples in Passage 6 had finite cells and a cell with good viability had a minimum value of 95%. This indicated that there were more living cells than dead cells and were able to effectively use the nutrients in the culture optimally 19. Kidney cell populations as in vitro model candidates for bioac-tive compounds and vaccine development assay must be validated against specific molecular markers that support their physiological function.…”

mentioning

confidence: 99%

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Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Yusni

Mariya

Saepuloh

et al. 2023

J of Medical Primatology

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Background Cell culture is the proliferation of a cell population in vitro by isolating from the original tissue or growing from existing ones. One essential source is the monkey kidney cell cultures which have an essential role in biomedical study. This is due to the significant homology between the human and macaque genomes making these useful for cultivating human viruses, especially enteroviruses, and growing vaccines. Methods This study developed cell cultures derived from the kidney of Macaca fascicularis (Mf) and validated its gene expression. Results The primary cultures were successfully subcultured up to six passages, grew as monolayers, and exhibited epithelial‐like morphology. The cultured cells remained heterogeneous in phenotype and they expressed CD155 and CD46 as viral receptors, cell morphology (CD24, endosialin, and vWF), proliferation, also apoptosis markers (Ki67 and p53). Conclusions These results indicated that the cell cultures can be used as in vitro model cells for vaccine development and bioactive compound.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Vero Cell Linementioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Yusni

Mariya

Saepuloh

et al. 2023

J of Medical Primatology

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“…3D cell culture and tissue validation are complicated due to a confluence of factors. More often 3D tissues are not compatible with existing viability assays, hence to obtain information that is relevant, consistent and reproducible, validation of results is crucial [251, 252].…”

Section: Choosing the Right Imaging Toolmentioning

confidence: 99%

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Hilzenrat

Gill

McArthur

2022

Journal of Biophotonics

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The past decade has seen an increasing demand for more complex, reproducible and physiologically relevant tissue cultures that can mimic the structural and biological features of living tissues. Monitoring the viability, development and responses of such tissues in real‐time are challenging due to the complexities of cell culture physical characteristics and the environments in which these cultures need to be maintained in. Significant developments in optics, such as optical manipulation, improved detection and data analysis, have made optical imaging a preferred choice for many three‐dimensional (3D) cell culture monitoring applications. The aim of this review is to discuss the challenges associated with imaging and monitoring 3D tissues and cell culture, and highlight topical label‐free imaging tools that enable bioengineers and biophysicists to non‐invasively characterise engineered living tissues.

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Single cell analysis of Chinese hamster ovary cells during a bioprocess using a novel dynamic imaging system

Breen,

Flynn,

Bergin

et al. 2024

Biotechnology Progress

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Reliable monitoring of mammalian cells in bioreactors is essential to biopharmaceutical production. Trypan blue exclusion is a method of determining cell density and viability that has been used for over one hundred years to monitor cells in culture and is the current standard method in biomanufacturing. This method has many disadvantages however and there is a growing demand for more detailed and in‐line measurements of cell growth in bioreactors. This article assesses a novel dynamic imaging system for single cell analysis. This data shows that comparable total cell density, viable cell density and percentage viability data shown here, generated by the imaging system, aligned well with conventional trypan blue counting methods for an industrially relevant Chinese Hamster Ovary (CHO) cell line. Furthermore, detailed statistical analysis shows that the classification system used by the PharmaFlow system can reveal trends of interest in monitoring the health of mammalian cells over a 6‐day bioreactor culture. The system is also capable of sampling at‐line, removing the necessity for taking samples off‐line and enabling real time monitoring of cells in a bioreactor culture.

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Surveilling cellular vital signs: toward label-free biosensors and real-time viability assays for bioprocessing

Cited by 8 publications

References 51 publications

Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Kidney cell culture Macaca fascicularis as a candidate for vaccine development and in vitro model

Imaging approaches for monitoring three‐dimensional cell and tissue culture systems

Single cell analysis of Chinese hamster ovary cells during a bioprocess using a novel dynamic imaging system

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