Surveillance of SARS-CoV-2 in nine neighborhood sewersheds in Detroit Tri-County area, United States: Assessing per capita SARS-CoV-2 estimations and COVID-19 incidence

Li, Yabing; Miyani, Brijen; Zhao, Liang; Spooner, Maddie; Gentry, Zach; Zou, Yangyang; Rhodes, Geoff; Li, Hui; Kaye, Andrew; Norton, John; Xagoraraki, Irene

doi:10.1016/j.scitotenv.2022.158350

Cited by 9 publications

(14 citation statements)

References 52 publications

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“…Afterwards, supernatants containing viruses were separated by adjusting the pH and centrifugation. Finally, the supernatants containing viruses were passed through 0.45 μm and 0.22 μm Millipore filters (MilliporeSigma, Burlington, MA, United States), which were followed by aliquoting and storage of the final aliquots at -80°C for downstream molecular analysis (1)(2)(3)(4)20). Bacteriophage Phi6 was applied as a proxy virus to estimate the recovery efficiency during virus concentration (3,29,39).…”

Section: Virus Adsorption-elution Methodsmentioning

confidence: 99%

“…Following VIRADEL and PEG methods, viral RNA was extracted using the QIAamp Viral RNA kit (Qiagen, Germantown, MD, United States), following the manufacturer's protocol modified by use of 140 μL elution buffer to extract the viral RNA (1-4). RT-ddPCR was performed on a QX200 AutoDG Droplet Digital PCR system (Bio-Rad, Hercules, CA, United States), using the One-step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Hercules, CA, United States) as described previously (2,3). United States Centers for Disease Control and Prevention (US CDC) primers and probes that target the N1 and N2 genes of SARS-CoV-2 were used (2,3,13).…”

Section: Rna Extraction Rt-ddpcr Rt-qpcrmentioning

confidence: 99%

“…RT-ddPCR was performed on a QX200 AutoDG Droplet Digital PCR system (Bio-Rad, Hercules, CA, United States), using the One-step RT-ddPCR Advanced Kit for Probes (Bio-Rad, Hercules, CA, United States) as described previously (2,3). United States Centers for Disease Control and Prevention (US CDC) primers and probes that target the N1 and N2 genes of SARS-CoV-2 were used (2,3,13). N1 N2 gene Duplex Assay Reaction Mixture was reported previously (2,3,13).…”

Section: Rna Extraction Rt-ddpcr Rt-qpcrmentioning

confidence: 99%

“…United States Centers for Disease Control and Prevention (US CDC) primers and probes that target the N1 and N2 genes of SARS-CoV-2 were used (2,3,13). N1 N2 gene Duplex Assay Reaction Mixture was reported previously (2,3,13). Following the preparation of the Duplex Mixture and oil droplets generation, samples were run on a C1000 Touch Thermal Cycler (Bio-Rad, Hercules, CA, United States) using the thermocycling conditions which were reported previously (2,3,13).…”

Section: Rna Extraction Rt-ddpcr Rt-qpcrmentioning

confidence: 99%

“…Wastewater surveillance has been widely adopted by researchers and health agencies as an effective tool for tracking Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in wastewater amid the Coronavirus Disease 2019 (COVID- 19) pandemic (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13). SARS-CoV-2 was first identified in Wuhan, Hubei, China, and was designated a Public Health Emergency of International Concern on January 30th, 2020, by the World Health Organization (WHO).…”

Section: Introductionmentioning

confidence: 99%

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Targeting a free viral fraction enhances the early alert potential of wastewater surveillance for SARS-CoV-2: a methods comparison spanning the transition between delta and omicron variants in a large urban center

Zhao

Geng

Corchis-Scott

et al. 2023

Front. Public Health

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IntroductionWastewater surveillance has proven to be a valuable approach to monitoring the spread of SARS-CoV-2, the virus that causes Coronavirus disease 2019 (COVID-19). Recognizing the benefits of wastewater surveillance as a tool to support public health in tracking SARS-CoV-2 and other respiratory pathogens, numerous wastewater virus sampling and concentration methods have been tested for appropriate applications as well as their significance for actionability by public health practices.MethodsHere, we present a 34-week long wastewater surveillance study that covers nearly 4 million residents of the Detroit (MI, United States) metropolitan area. Three primary concentration methods were compared with respect to recovery of SARS-CoV-2 from wastewater: Virus Adsorption-Elution (VIRADEL), polyethylene glycol precipitation (PEG), and polysulfone (PES) filtration. Wastewater viral concentrations were normalized using various parameters (flow rate, population, total suspended solids) to account for variations in flow. Three analytical approaches were implemented to compare wastewater viral concentrations across the three primary concentration methods to COVID-19 clinical data for both normalized and non-normalized data: Pearson and Spearman correlations, Dynamic Time Warping (DTW), and Time Lagged Cross Correlation (TLCC) and peak synchrony.ResultsIt was found that VIRADEL, which captures free and suspended virus from supernatant wastewater, was a leading indicator of COVID-19 cases within the region, whereas PEG and PES filtration, which target particle-associated virus, each lagged behind the early alert potential of VIRADEL. PEG and PES methods may potentially capture previously shed and accumulated SARS-CoV-2 resuspended from sediments in the interceptors.DiscussionThese results indicate that the VIRADEL method can be used to enhance the early-warning potential of wastewater surveillance applications although drawbacks include the need to process large volumes of wastewater to concentrate sufficiently free and suspended virus for detection. While lagging the VIRADEL method for early-alert potential, both PEG and PES filtration can be used for routine COVID-19 wastewater monitoring since they allow a large number of samples to be processed concurrently while being more cost-effective and with rapid turn-around yielding results same day as collection.

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