Surveillance of molecular markers for antimalarial resistance in Zambia: Polymorphism of Pfkelch 13, Pfmdr1 and Pfdhfr/Pfdhps genes

Sitali, Lungowe; Mwenda, Mulenga; Miller, John M.; Bridges, Daniel J.; Moonga, Hawela; Hamainza, Busiku; Mudenda-Chilufya, Mutinta; Chizema-Kawesha, Elizabeth; Daniels, Rachel F.; Eisele, Thomas P.; Nerland, Audun Helge; Chipeta, James; Lindtjørn, Bernt

doi:10.1016/j.actatropica.2020.105704

Cited by 7 publications

(9 citation statements)

References 43 publications

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“…Recent studies have reported that the pfdhps 437G has been increasing. Studies across West and central Africa, in neighboring Nigeria and in Zambia have reported high prevalence of this mutation among clinical P. falciparum samples [ 26 , 45 , 46 ]. Similar findings were reported by Ndong-Ngomo et al.…”

Section: Discussionmentioning

confidence: 99%

Allelic frequencies of mutants of the Plasmodium falciparum, quinoline and folate metabolizing genes in the west region of Cameroon

Ali

Tchuenkam

Tagomo

et al. 2022

Heliyon

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Section: Discussionmentioning

confidence: 99%

Allelic frequencies of mutants of the Plasmodium falciparum, quinoline and folate metabolizing genes in the west region of Cameroon

Ali

Tchuenkam

Tagomo

et al. 2022

Heliyon

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“…The table in the article (doi: 10.1016/j.actatropica.2020.105704 ) related to this data in brief [1] as supplementary information, shows the frequencies of the Pfdhfr, Pfdhps and Pfmdr1 single nucleotide polymorphisms for the samples from Southern and Western Provinces. The most prevalent mutant alleles observed were: Pfdhps 437G (87.7%), followed by Pfdhfr 59R (81.3%), 51I (66.7%) and 108N (66.8%).…”

Section: Data Descriptionmentioning

confidence: 99%

Data on selected antimalarial drug resistance markers in Zambia

et al. 2021

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This article describes data on selected resistance markers for antimalarial drugs used in Zambia. Antimalarial drug resistance has hindered the progress in the control and elimination of malaria. Blood samples were collected during a cross-sectional household survey, conducted during the peak malaria transmission, April to May of 2017. Dried blood spots were collected during the survey and transported to a laboratory for analysis. The analysed included polymerase chain reaction (PCR) followed by high resolution melt (HRM) for mutations associated with Sulfadoxine-pyrimethamine resistance in the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) and P. falciparum dihydropteroate synthase (Pfdhps) genes. Mutations associated with artemether-lumefantrine resistance in falciparum multi-drug resistance gene 1 ( Pfmdr1 ) were also assessed using PCR and HRM analysis, whereas the P. falciparum Kelch 13 ( PfK13 ) gene was assessed using nested PCR followed by amplicon sequencing.

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“…SP is still employed for IPTp. 23 The emergence and spread of antimalarial drug resistance in neighbouring countries, particularly artemisinin partial resistance, 18 , 24 is an additional threat to national malaria control and elimination. In areas of low transmission, premunition will decrease 25 and may enhance the emergence of drug resistance, as a greater proportion of infected individuals seek treatment thereby exposing the parasite population to greater drug pressure.…”

Section: Introductionmentioning

confidence: 99%

“…While validated mutations associated with ART-R in the K13 gene have not been detected, parasites carrying mutations that confer SP resistance are highly prevalent in the country. 23 , 29 Therefore, serial or continuous collections of samples can help us better understand trends in antimalarial drug resistance. Here, we successfully genotyped 389 P. falciparum samples collected from 2012–2018 from 8 health centres in Choma District, a low malaria transmission zone in, Southern Province, Zambia, using 815 molecular inversion probes (MIPs) targeting 14 key P. falciparum drug resistance genes to determine temporal trends in resistance mutations.…”

Section: Introductionmentioning

confidence: 99%

Temporal genomic analysis ofPlasmodium falciparumreveals increased prevalence of mutations associated with delayed clearance following treatment with artemisinin-lumefantrine in Choma District, Southern Province, Zambia

Fola,

Kobayashi,

Shields

et al. 2024

Preprint

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The emergence of antimalarial drug resistance is an impediment to malaria control and elimination in Africa. Analysis of temporal trends in molecular markers of resistance is critical to inform policy makers and guide malaria treatment guidelines. In a low and seasonal transmission region of southern Zambia, we successfully genotyped 85.5% (389/455) ofPlasmodium falciparumsamples collected between 2013-2018 from 8 spatially clustered health centres using molecular inversion probes (MIPs) targeting key drug resistance genes. Aside from one sample carrying K13 R622I, none of the isolates carried other World Health Organization-validated or candidate artemisinin partial resistance (ART-R) mutations in K13. However, 13% (CI, 9.6-17.2) of isolates had the AP2MU S160Nmutation, which has been associated with delayed clearance following artemisinin combination therapy in Africa. This mutation increased in prevalence between 2015-2018 and bears a genomic signature of selection. During this time period, there was an increase in the MDR1 NFD haplotype that is associated with reduced susceptibility to lumefantrine. Sulfadoxine-pyrimethamine polymorphisms were near fixation. While validated ART-R mutations are rare, a mutation associated with slow parasite clearance in Africa appears to be under selection in southern Zambia.

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Surveillance of molecular markers for antimalarial resistance in Zambia: Polymorphism of Pfkelch 13, Pfmdr1 and Pfdhfr/Pfdhps genes

Cited by 7 publications

References 43 publications

Allelic frequencies of mutants of the Plasmodium falciparum, quinoline and folate metabolizing genes in the west region of Cameroon

Allelic frequencies of mutants of the Plasmodium falciparum, quinoline and folate metabolizing genes in the west region of Cameroon

Data on selected antimalarial drug resistance markers in Zambia

Temporal genomic analysis ofPlasmodium falciparumreveals increased prevalence of mutations associated with delayed clearance following treatment with artemisinin-lumefantrine in Choma District, Southern Province, Zambia

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