Surveillance of extended-spectrum β-lactamases from clinical samples and faecal carriers in Barcelona, Spain

Miró, Elisenda; Mirelis, Beatriz; Navarro, Ferrán; Rivera, Alba; Mesa, Raúl Jesús; Roig, Ma Carme; Gómez, Laura; Coll, Pere

doi:10.1093/jac/dki395

Cited by 70 publications

(41 citation statements)

References 9 publications

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“…All ESBLs found were of the CTX-M-9, CTX-M-10, and CTX-M-14 types (29). However, in another fecal carrier study from Barcelona (2001 and, CTX-M-15 was detected in five cases, as were both CTX-M-9 and CTX-M-14 (17). The data reported here indicate that dissemination of highlevel cefotaxime-and ceftazidime-resistant E. coli may be attributable to the following.…”

Section: Discussionmentioning

confidence: 90%

“…No clonal dissemination of ESBL-producing E. coli was observed in a nationwide study of 40 Spanish centers in 2000 (9). CTX-M-15 was not detected in any of the three clinical Spanish studies mentioned (9,17,25). Also, CTX-M-15 was not detected in one study of fecal carriers performed in Madrid in 2003 (29).…”

Section: Discussionmentioning

confidence: 91%

“…At present, the more prevalent CTX-M-types isolated from clinical samples in Spain have been CTX-M-9 and the genetically related CTX-M-14, followed by CTX-M-10 (9,17,25). No clonal dissemination of ESBL-producing E. coli was observed in a nationwide study of 40 Spanish centers in 2000 (9).…”

Section: Discussionmentioning

confidence: 95%

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Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions

et al. 2006

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A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated strains. The principal cluster was isolated from urine, wound, blood, and other samples in three hospitals, eight nursing homes, and a community healthcare center. This cluster was associated with both nosocomial (65%) and community-acquired (35%) infections. Most strains were resistant to ciprofloxacin, gentamicin, tobramycin, cefepime, amoxicillin-clavulanic acid, and trimethoprim-sulfamethoxazole but were susceptible to imipenem. All isolates from the four clusters expressed the extended-spectrum ␤-lactamase (ESBL) CTX-M-15. This enzyme was also present in 8 (30.8%) of the 26 unrelated isolates. The other ESBLs, CTX-M-14 and CTX-M-32, were detected in five and seven cases, respectively, but they were detected in individual E. coli isolates only. In three clusters, bla CTX-M-15 alleles were linked to an ISEcp1-like element, while in eight strains of cluster II an IS26 element preceded the bla CTX-M-15 allele. An additional pool of resistance genes included tetA, drfA14 or dfrA17, sul1 or sul2, aac(6)Ib, and aac(3)IIb. All except one of the 27 isolates tested for genetic virulence markers harbored the same three virulence genes: iutA and fyuA (siderophores), and traT (serum survival factor). Epidemic or occasional isolates of cefotaxime-and ceftazidime-resistant E. coli can spread between distinct health facilities including hospitals, community health centers, and long-term care centers.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 91%

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Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions

et al. 2006

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“…For years, PER ␤-lactamases were thought to be significant only in Turkey (PER-1) and Argentina (PER-2) (4, 12, 32, 41, 50, 51). However, the recent identification of PER-1 producers in several European countries and in the Far East suggests their proceeding dissemination (20,27,33,35,46,54). In Korea, as in Turkey, PER-1 production by Acinetobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

et al. 2007

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Forty-one Pseudomonas aeruginosa isolates with extended-spectrum ␤-lactamases (ESBLs) from a hospital in Warsaw, Poland, were analyzed. Thirty-seven isolates from several wards were collected over 9 months in 2003 and 2004. The isolates were recovered from patients with multiple types of infections, mostly respiratory tract and postoperative wound infections. All 41 isolates produced the PER-1 ESBL, originally observed in Turkey but recently also identified in several countries in Europe and the Far East. The bla PER-1 gene resided within the Tn1213 composite transposon, which was chromosomally located. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) revealed the presence of three separate clones among the isolates. Two of these, corresponding to sequence types (STs) ST244 and ST235, were responsible for parallel outbreaks. Apart from PER-1, all the isolates produced OXA-2 oxacillinase. ST235 isolates additionally expressed a novel enzyme, OXA-74, differing by one amino acid from the OXA-17 ESBL identified originally in PER-1-and OXA-2-positive P. aeruginosa isolates from Ankara, Turkey, in 1992. These earlier Ankara isolates with PER-1, OXA-2, and OXA-17 were also classified into ST235, which is a single-locus variant of two other STs, ST227 and ST230. ST227, ST230, and ST235 all correspond to the recently described clonal complex BG11, which seems to be internationally distributed, having spread in Turkey, Greece, Italy, Hungary, Poland, Sweden, and much of Russia. It is associated with various ␤-lactamases, including PER-1 and VIM metalloenzymes. This work further demonstrates the value of MLST of P. aeruginosa.

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“…Although microorganisms with PER-1 expression have been found to predominate in Turkey, outbreaks induced by P. aeruginosa strains have been reported from Italy [5], where PER-1 has also been detected in P. mirabilis and Alcaligenes faecalis [23,24]. Likewise, the presence of this enzyme has also been noted in France and Spain [25,26]. PER-1 producing Acinetobacter baumannii has been reported from France, Belgium, Romania, Russia and Hungary [10,19,[27][28][29].…”

Section: Discussionmentioning

confidence: 99%

Susceptibility, phenotypes of resistance, and extended-spectrum β-lactamases in Acinetobacter baumannii strains

Sacha

Wieczorek

Ojdana

et al. 2012

Folia Histochem Cytobiol.

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Abstract:Acinetobacter baumannii plays an increasing role in the pathogenesis of infections in humans. The bacilli are frequently isolated from patients treated in intensive care units. A growing resistance to antibiotics is leading to the emergence of strains that are multidrug-resistant and resistant to all available agents. The objective of this study was to assess susceptibility to antibiotics and to determine the presence and current level of the extended-spectrum b-lactamases (ESBLs) and attempt to isolate the Acinetobacter baumannii strain carrying the bla PER gene. A total of 51 strains of A. baumannii identified by phenotypic features were examined. That the strains belonged to the species was confirmed by the presence of the bla OXA-51-like gene. A broth microdilution method was used for antibacterial susceptibility testing. The occurrence of ESBLs was determined using phenotypic double-disk synergy tests. The PCR technique was used to confirm the presence of the bla PER-1 gene encoding ESBL. The most active antibiotics were meropenem, cefepime and ampicillin/sulbactam, with susceptibility shown by 76.5%, 60.8% and 56.9% of the strains, respectively. The strains exhibited the highest resistance (> 75%) to piperacillin, tetracycline, ciprofloxacin and cefotaxime. Phenotypic tests revealed ESBL mechanism of resistance in approximately 20% of Acinetobacter baumannii isolates. However, the PCR technique did not confirm the presence of the bla PER-1 gene in any of the Acinetobacter baumannii strains examined in our hospital. Acinetobacter baumannii strains demonstrate considerable resistance to many groups of antibiotics. Our findings indicate the involvement of enzymes belonging to families other than PER b-lactamase in resistance to b-lactams in

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Surveillance of extended-spectrum β-lactamases from clinical samples and faecal carriers in Barcelona, Spain

Cited by 70 publications

References 9 publications

Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions

Spread of Escherichia coli Strains with High-Level Cefotaxime and Ceftazidime Resistance between the Community, Long-Term Care Facilities, and Hospital Institutions

Outbreak of Pseudomonas aeruginosa Infections with PER-1 Extended-Spectrum β-Lactamase in Warsaw, Poland: Further Evidence for an International Clonal Complex

Susceptibility, phenotypes of resistance, and extended-spectrum β-lactamases in Acinetobacter baumannii strains

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