Surveillance of Escherichia coli in different types of chicken and duck hatcheries: one health outlook

Yousef, Hend M.Y.; Hashad, Mahmoud E.; Osman, Kamelia M.; Alatfeehy, Nayera M.; Hassan, Wafaa M.M.; Elebeedy, Lamia A.; Salem, Heba M.; Shami, Ashwag; Al-Saeed, Fatimah A.; El-Saadony, Mohamed T.; El-Tarabily, Khaled A.; Marouf, Sherif

doi:10.1016/j.psj.2023.103108

Cited by 6 publications

(6 citation statements)

References 55 publications

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“…Antibiotics have been used for decades to manage APEC; but, due to the rise of multi-drug resistant E. coli and the challenge of developing novel antimicrobial medicines, vaccination has emerged as the most effective means of controlling E. coli infections on poultry farms [5,44]. In this study, the antibiotic sensitivity testing exposed that all isolates were sensitive to Doxycycline and 10 % resistant to both Neomycin and Ciprofloxacin.…”

Section: Discussionmentioning

confidence: 90%

“…E. coli is a serious avian pathogen that contributes a significant hazard to all avian species [5]. For E. coli identification, both phenotypic and genotypic approaches are essential [5].…”

Section: Discussionmentioning

confidence: 99%

“…coli is a serious avian pathogen that contributes a significant hazard to all avian species [5]. For E. coli identification, both phenotypic and genotypic approaches are essential [5]. In this study, pathogenic E. coli was recovered from 30 different broiler chicken flocks located in Giza, Behaira and El-Sharqiyah governorates in Egypt with a recovery rate 50% (15/30).…”

Section: Discussionmentioning

confidence: 99%

“…Symptoms of E. coli infection in broiler chickens may vary depending on the virulence of the pathogen, bird immunity and surrounding environment [5]. Numerous factors can increase the risk of E. coli infection in broiler chickens including overcrowding, poor ventilation, high stocking density, inadequate sanitation practices, and immunosuppressive diseases [6].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Ahmed,

Salem,

Hamoud

et al. 2024

Egyptian Journal of Veterinary Sciences

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scherichia coli (E. coli) is a widespread avian pathogen and usually is considered as a secondary pathogen for different infectious agents causing huge financial losses in the poultry industry. Thus, the current works aim to molecular detection of resistance and pathogenicity genes of E. coli isolated from broiler chicken farms. A total of 18 flocks out of 30 broiler flocks (60%) were positive for E. coli infection. On the base of the Congo red (CR) binding assay, 15 flocks out of 18 were identified as pathogenic E. coli (83.3%) and 3 flocks appeared as nonpathogenic E. coli (16.6%). All pathogenic E. coli were subjected to in vitro antibiotic sensitivity testing to select the most resistant isolates. Ten pathogenic E. coli isolates representing ten different broiler flocks were subjected for molecular identification via polymerase chain reaction (PCR) for both resistance and virulence genes. The most predominant isolated serotypes were O91, O128, O78, O124, O 2 and O44. These strains were related to EHEC, EPEC, ETEC, and EIEC. These E. coli isolates are multidrug resistant (MDR) to extensively drug-resistant (XDR). The virulence genes tsh, papC, iss, iutA, and hlyF were detected in 50% of isolates having 5 genes and 50% having 4 genes. All the tested isolates showed MDR genes 60% of isolates were positive for 5 resistance genes and 20% were positive for 4 resistance genes and 20% were positive for resistance 3 genes. We can conclude that E. coli continues to threaten poultry industry and further studies are recommended to found safe antibiotic natural alternatives to overcome E. coli MDR existing strains side by side with strengthening the bird's immunity and application of strict hygienic measures.

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Section: Discussionmentioning

confidence: 90%

“…E. coli is a serious avian pathogen that contributes a significant hazard to all avian species [5]. For E. coli identification, both phenotypic and genotypic approaches are essential [5].…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Ahmed,

Salem,

Hamoud

et al. 2024

Egyptian Journal of Veterinary Sciences

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“…Measures to prevent APEC infection can be carried out by vaccination with respiratory agents, reducing stress in chickens, predisposing factors, and treatment with antibiotics (Adrenalin et al 2020). The use of antibiotics in animal production has a major impact on public health, increasing the incidence of antibiotic and multidrug resistance (Saha et al 2020;Effendi et al 2021;Yousef et al 2023). Resistance events can be transmitted to other animals and humans through direct contact, production of animal products, and indirectly through the environment (Vidovic and Vidovic 2020;Tyasningsih et al 2022;Faridah et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia

KENDEK,

PUTRI,

WIBISONO

et al. 2024

Biodiversitas

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Abstract. Kendek IA, Putri MFR, Wibisono FJ, Effendi MH, Tyasningsih W, Ugbo EN, Agumah NB. 2024. Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia. Biodiversitas 25: 1246-1252. Avian Pathogenic Escherichia coli (APEC) is a strain of pathogen that can cause colibacillosis in poultry, including ducks worldwide. This disease can be influenced by various virulence genes, one of which is the hlyF gene which can cause systemic disorders and potentially become zoonotic in poultry. This study aimed to detect the hlyF virulence gene from the multidrug resistance (MDR) properties of Escherichia coli originating from 158 duck cloacal swab samples from seven live markets in Surabaya. Samples were isolated and identified using Eosin Methylene Blue Agar (EMBA) and MacConkey Agar (MCA) media, Gram staining, and then continued with biochemical tests of Triple Sugar Iron Agar (TSIA), Simmons Citrate Agar (SCA), Sulfide Indole Motility (SIM), Methyl red (MR) and Voges-Proskauer (VP). Samples were tested for MDR properties and continued with confirmation of the hlyF virulence gene using PCR. Based on the results of this study, it showed that 85% (134/158) were positive for Escherichia coli. Escherichia coli bacteria experienced resistance to the antibiotic erythromycin 96% (129/134), ciprofloxacin 16% (22/134), antibiotics gentamicin 15% (20/134), aztreonam 4.4% (6/134) and chloramphenicol 3% (5/58). The MDR test results in this study were 15% (20/134), while the PCR test results for the hlyF gene were 60% (12/20). Therefore, Escherichia coli was found to have the hlyF virulence gene, an MDR in Escherichia coli bacteria at the Surabaya live market. Furthermore, APEC strains with the hlyF gene, an MDR, potentially affect public health.

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Revolutionizing poultry hygiene: advanced electrostatic and cold fog disinfection strategies combat Mycoplasma gallisepticum in hatching eggs

Kamal,

Kaoud,

Salem

et al. 2024

Poultry Science

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Surveillance of Escherichia coli in different types of chicken and duck hatcheries: one health outlook

Cited by 6 publications

References 55 publications

Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Molecular detection of hlyF gene on multidrug resistance of avian pathogenic Escherichia coli isolated from ducks on wet markets of Surabaya, Indonesia

Revolutionizing poultry hygiene: advanced electrostatic and cold fog disinfection strategies combat Mycoplasma gallisepticum in hatching eggs

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