Surveillance of Bulk Raw and Commercially Pasteurized Cows' Milk from Approved Irish Liquid-Milk Pasteurization Plants To Determine the Incidence of
            <i>Mycobacterium paratuberculosis</i>

O’Reilly, Ciara E.; O’Connor, Lisa; Anderson, Wayne A.; Harvey, P.; Grant, Irene R.; Donaghy, John; Rowe, M.T.; O'mahony, P.

doi:10.1128/aem.70.9.5138-5144.2004

Cited by 71 publications

(45 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…paratuberculosis among high levels of other environmental bacteria is beneficial (7). Such samples include water (31) and bulk milk tank (12,21) samples as well as other environmental samples (8,25). …”

mentioning

confidence: 99%

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

Bannantine

Radosevich

Stabel

et al. 2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

Bannantine

Radosevich

Stabel

et al. 2007

Clin Vaccine Immunol

View full text Add to dashboard Cite

show abstract

“…paratuberculosis DNA as determined by PCR, suggesting that the current pasteurization protocols may be effective in controlling M. avium subsp. paratuberculosis found in milk (16,33). As long as the public impact of M. avium subsp.…”

mentioning

confidence: 99%

Development of an F57 Sequence-Based Real-Time PCR Assay for Detection ofMycobacterium aviumsubsp.paratuberculosisin Milk

Tasara

Stephan

2005

Appl Environ Microbiol

View full text Add to dashboard Cite

A light cycler-based real-time PCR (LC-PCR) assay that amplifies the F57 sequence of Mycobacterium avium subsp. paratuberculosis was developed. This assay also includes an internal amplification control template to monitor the amplification conditions in each reaction. The targeted F57 sequence element is unique for M. avium subsp. paratuberculosis and is not known to exist in any other bacterial species. The assay specificity was demonstrated by evaluation of 10 known M. avium subsp. paratuberculosis isolates and 33 other bacterial strains. The LC-PCR assay has a broad linear range (2 ؋ 10 1 to 2 ؋10 6 copies) for quantitative estimation of the number of M. avium subsp. paratuberculosis F57 target copies in positive samples. To maximize the assay's detection sensitivity, an efficient strategy for isolation of M. avium subsp. paratuberculosis DNA from spiked milk samples was also developed. The integrated procedure combining optimal M. avium subsp. paratuberculosis DNA isolation and real-time PCR detection had a reproducible detection limit of about 10 M. avium subsp. paratuberculosis cells per ml when a starting sample volume of 10 ml of M. avium subsp. paratuberculosis-spiked milk was analyzed. The entire process can be completed within a single working day and is suitable for routine monitoring of milk samples for M. avium subsp. paratuberculosis contamination. The applicability of this protocol for naturally contaminated milk was also demonstrated using milk samples from symptomatic M. avium subsp. paratuberculosis-infected cows, as well as pooled samples from a dairy herd with a confirmed history of paratuberculosis.

show abstract

“…Many authors estimate that raw bulk tank milk could reach levels of up to 4 and 5 log CFU/ml of M. avium subsp. paratuberculosis cells (11,13,14,16,21,25,34). This is mostly due to fecal contamination and also, to a lesser extent, to cells secreted within the udders (5, 33, 35); Sweeney et al (35) found 2 to 8 CFU/ml (0.3 to 0.9 log CFU/ml) of M. avium subsp.…”

mentioning

confidence: 99%

Inactivation ofMycobacterium aviumsubsp.paratuberculosisin Cow's Milk by Means of High Hydrostatic Pressure at Mild Temperatures

López-Pedemonte

Sevilla

Garrido

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Surveillance of Bulk Raw and Commercially Pasteurized Cows' Milk from Approved Irish Liquid-Milk Pasteurization Plants To Determine the Incidence of Mycobacterium paratuberculosis

Cited by 71 publications

References 18 publications

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

Production and Characterization of Monoclonal Antibodies against a Major Membrane Protein ofMycobacterium aviumsubsp.paratuberculosis

Development of an F57 Sequence-Based Real-Time PCR Assay for Detection ofMycobacterium aviumsubsp.paratuberculosisin Milk

Inactivation ofMycobacterium aviumsubsp.paratuberculosisin Cow's Milk by Means of High Hydrostatic Pressure at Mild Temperatures

Contact Info

Product

Resources

About