Surveillance of Antimicrobial Susceptibility Patterns among Shigella Species Isolated in China during the 7-Year Period of 2005-2011

Yang, Haifei; Chen, Guosheng; Yu, Zhao; Liu, Yanyan; Cheng, Jun; Hu, Lifen; Yao, Yuqi; Li, Jiabin

doi:10.3343/alm.2013.33.2.111

Cited by 26 publications

(26 citation statements)

References 15 publications

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“…Moderate resistance to tetracycline (45.9 %) and cefotaxime (29.7 %) was also observed; however, resistance to chloramphenicol and ciprofloxacin was relatively low (16.2 and 2.7 %, respectively) [20]. In Beijing, Salmonella and Shigella showed resistance to ciprofloxacin isolateed from diarrheal children 9.3% and 29.7%, respectively [21]. Salmonella and Shigella isolates showed resistance rates to CIP 9.2% and 2.7% at Shanghai and Anhui in China [22].…”

Section: Results Of Prevalence For Bacterial Isolates In Stool Samplementioning

confidence: 99%

Occurrence and antibiogram of enteric bacterial isolates from stool samples of gastroenteritis children under 5 years of age in district Faisalabad, Pakistan

Masood¹,

Abdullah²,

Anam³

et al. 2019

PAB

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Occurrence and Antibiogram of enteric bacterial isolates from stool samples of gastroenteritis children under 5 years of age in district Faisalabad, Pakistan. AbstractAcute gastroenteritis is accompanied by severe bloody diarrhea, hemolytic uremic syndrome and acute renal impairment. It is one of leading cause of morbidity and mortality in children, worldwide. Annually, acute gastroenteritis is associated with 2 million deaths in children under 5 years of age. The present study aimed to determine the occurrence and antibiotic susceptibility profiling of enteric bacterial pathogens isolated from stool samples of gastroenteritis patients. A total of 200 stool samples were collected from DHQ hospital Faisalabad followed by enrichment in Selenite F and nutrient broth media. Post 24 hours incubation at 37 o C, cultures were streaked on various selective and differential media. Bacterial isolates were first identified by morphological and microscopic examination and then confirmed by biochemical tests. Isolated bacteria were tested for antibiotic sensitivity by Kirby's Bauer disc diffusion method. Our results indicated the presence of three major bacterial isolates with Escherichia coli (E. coli) showing highest prevalence rate (78%), followed by Salmonella Typhi (55%) and Shigella dysenteriae (36%). E. coli was most sensitive (80%) to enrofloxacin and least sensitive to ciprofloxacin (10%). S. Typhi isolates were also most sensitive to enrofloxacin (80%), while most resistant to cefotaxime (90%). About 95% of Shigella dysentriae isolates were resistant to amoxicillin (95%), while highest sensitivity (80%) was found against ofloxacin. E.coli was the most prevalent bacteria in enteritis patients. Results of antibiotic sensitivity suggest combination antibiotic therapy (enrofloxacin+ ofloxacin) should be prescribed to treat enteritis infections in children. These findings will be helpful in diagnosing and determining the course of antibiotic therapy to treat children with gastroenteritis.

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Section: Results Of Prevalence For Bacterial Isolates In Stool Samplementioning

confidence: 99%

Occurrence and antibiogram of enteric bacterial isolates from stool samples of gastroenteritis children under 5 years of age in district Faisalabad, Pakistan

Masood¹,

Abdullah²,

Anam³

et al. 2019

PAB

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“…Similar results have been reported in Burkina Faso and Nigeria (Bawa et al, 2016;Fashae et al, 2010). Multidrug resistance to these old and commonly used antimicrobials is a public health problem and could facilitate usage of fluoroquinolones and third-generation cephalosporins for empiric treatment (Yang et al, 2013). Salmonella Paratyphi B was the most resistant of the serotypes isolated from human isolates to urban and rural sites and exhibited resistance to tetracycline, ticarcillin, ampicillin, amoxicillin, amoxicillin/cluvulanic-acid and trimethoprime/sulfamexazol.…”

Section: Discussionmentioning

confidence: 99%

Serotyping and Antimicrobial Drug Resistance of Salmonella Isolated From Lettuce and Human Diarrhea Samples in Burkina Faso

Siourimè

Isidore

Traoré

et al. 2017

AJID

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Background: In Burkina Faso dirty water in particular those of the stoppings and the gutter ones are used for vegetables irrigation in the gardens. The aim of this study was to determine the prevalence and antibiotic susceptibility of Salmonella serotypes from humans and lettuce samples in Burkina Faso. Materials and Methods: Salmonella strains isolated from patients in 2009 to 2015 and lettuce samples in 2014 in Burkina Faso were serotyped using specific antisera. All strains were subjected to a set of 14 antibiotics to study their antibiogram by using Baeur-Kirby disk diffusion method. Results: Out of 154 Salmonella isolated, 60 were from human and 94 from lettuce samples. Serotyping revealed four different serotypes and 39% (60) untypeable strains from human and lettuce (14 and 46 strains). Salmonella serotypes from human and lettuce samples were: Paratyphi A (10% and 22%), Paratyphi B (34% and 8%), Paratyphi C (14% and 18%) and Typhi (21% and 1%). A high resistance of Salmonella Paratyphi B and Salmonella spp to tetracycline were 70% from human and 35 % from lettuce samples. Multiresistance was observed to tetracycline, chloramphenicol and amoxicillin/clavulanic-acid or ampicillin with Salmonella Paratyphi B 35% and Salmonella Typhi 33% from human samples and Salmonella spp 4% from lettuce samples. Conclusion:This study showed the diversity of Salmonella serotypes from both clinical and environmental samples and emergence of multiresistant Salmonella to antibiotics in Burkina Faso. A lettuce is a potential source of transmission of Salmonella causing diarrhea among human in Burkina Faso.

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“…The production of ESBLs is the major defense mechanism against third-generation cephalosporins employed by Enterobacteriaceae, including Shigella species. Recent reports from Jiangsu and Anhui Provinces located in eastern China, which neighbor Shanghai, showed that the rates of resistance to CTX were 21.5 % and 26.4 %, respectively, among Shigella isolates [14,15]. Therefore, the high prevalence of ESBL-producing Shigella isolates in eastern China is a threat to public health, and tracking and monitoring of the rapid spread of ESBL producers is urgently required.…”

Section: Discussionmentioning

confidence: 96%

Molecular characterization of the extended-spectrum beta-lactamase (ESBL)-producing Shigella spp. in Shanghai

et al. 2014

Eur J Clin Microbiol Infect Dis

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Shigellosis is a public health concern in China. We tested 216 Shigella isolates collected in Shanghai in 2007 for the production of extended-spectrum beta-lactamases (ESBLs). ESBL-producing isolates were characterized using polymerase chain reaction (PCR)-based genotyping, conjugation, pulsed-field gel electrophoresis (PFGE), and DNA sequence analysis of regions adjacent to bla genes. Plasmids containing genes encoding ESBLs were analyzed using plasmid replicon typing. ESBLs were produced by 18.1 % (39/216) of Shigella isolates, and all 39 ESBL-producing strains harbored bla CTX-M genes. CTX-M-14 was the most frequent variant (69.2 %, 27/39), followed by CTX-M-15 (15.4 %, 6/39). All bla CTX-M genes were transferable by conjugation, and the insertion sequence ISEcp1 was detected upstream of all bla CTX-M genes. The CTX-M-producing Shigella isolates showed high clonal diversity. IncI1, IncFII, IncN, and IncB/O replicons were respectively detected in 23 (58.9 %), 9 (23.1 %), 1 (2.6 %), and 1 (2.6 %) of the 39 transconjugants carrying bla CTX-M. The bla CTX-M-14 genes were most frequently carried by IncI1 (n = 13, 48.1 %) or IncFII (n = 9, 33.3 %) plasmids, and the bla CTX-M-15 genes were closely associated with IncI1 (n = 5, 83.3 %). Our findings demonstrate the high prevalence of ESBL-producing Shigella in Shanghai, the importance of plasmids and ISEcp1 as carriers of bla CTX-M genes, and the close association between certain bla CTX-M genes with a specific plasmid.

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Surveillance of Antimicrobial Susceptibility Patterns among Shigella Species Isolated in China during the 7-Year Period of 2005-2011

Cited by 26 publications

References 15 publications

Occurrence and antibiogram of enteric bacterial isolates from stool samples of gastroenteritis children under 5 years of age in district Faisalabad, Pakistan

Occurrence and antibiogram of enteric bacterial isolates from stool samples of gastroenteritis children under 5 years of age in district Faisalabad, Pakistan

Serotyping and Antimicrobial Drug Resistance of Salmonella Isolated From Lettuce and Human Diarrhea Samples in Burkina Faso

Molecular characterization of the extended-spectrum beta-lactamase (ESBL)-producing Shigella spp. in Shanghai

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