Surveillance of antibiotic resistance in &lt;i&gt;Escherichia coli&lt;/i&gt; isolated from wild cranes on the Izumi plain in Kagoshima prefecture, Japan

Suenaga, Yasuhiro; Obi, Takeshi; Ijiri, Moe; Chuma, Takehisa; Fujimoto, Yoshikazu

doi:10.1292/jvms.19-0305

Cited by 8 publications

(5 citation statements)

References 17 publications

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“…The E. coli isolated from wild and domestic animals in the area around the Salakphra Wildlife Sanctuary were resistant to ten antibiotics. Antibiotic resistance in commensal E. coli has been reported in various wild mammals worldwide, including birds [40][41][42][43], rodents [41], ungulates [44], primates [45,46], boars [47], foxes [48], rabbits [48,49], and deer [48,50]. We found that E. coli isolates from deer were resistant to AMP.…”

Section: Discussionmentioning

confidence: 72%

Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand

et al. 2022

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Background and Aim: Domestic and wild animals are important reservoirs for antibiotic-resistant bacteria. This study aimed to isolate Escherichia coli from feces of domestic and wild animals at an agricultural land interface area of Salaphra Wildlife Sanctuary, Thailand, and study the phylogenic characteristics and antibiotic resistance in these isolates. Materials and Methods: In this cross-sectional, descriptive study, we randomly collected ground feces from free-ranging wild animals (deer and elephants) and domestic animals (cattle and goats). All fecal samples were inoculated onto MacConkey agar plates, and lactose-fermenting colonies were identified as E. coli. Antibiotic susceptibility of the E. coli isolates was determined using the disc diffusion method. Polymerase chain reaction assays were used to detect antibiotic resistance and virulence genes. Results: We obtained 362 E. coli isolates from the collected fecal samples. The E. coli isolates were categorized into four phylogenetic groups according to the virulence genes (chuA, vjaA, and TspE4C2). Phylogenetic Group D was predominant in the deer (41.67%) and elephants (63.29%), whereas phylogenetic Group B1 was predominant in the cattle (62.31%), and phylogenetic Groups A (36.36%) and B2 (33.33%) were predominant in the goats. Antibiotic susceptibility testing revealed that most antibiotic-resistant E. coli were isolated from domestic goats (96.96%). Among the 362 E. coli isolates, 38 (10.5%) were resistant to at least one antibiotic, 21 (5.8%) were resistant to two antibiotics, and 6 (1.66%) were resistant to three or more antibiotics. Ampicillin (AMP) was the most common antibiotic (48.48%) to which the E. coli were resistant, followed by tetracycline (TET) (45.45%) and trimethoprim-sulfamethoxazole (3.03%). One isolate from an elephant was resistant to five antibiotics: AMP, amoxicillin, sulfisoxazole, TET, and ciprofloxacin. Determination of antibiotic resistance genes confirmed that E. coli isolates carried antibiotic resistance genes associated with phenotypic resistance to antibiotics. Most antibiotic-resistant E. coli belonged to phylogenic Groups A and B1, and most non-resistant E. coli belonged to phylogenic Groups B2 and D. Conclusion: Monitoring E. coli isolates from wild and domestic animals showed that all four phylogenic groups of E. coli have developed antibiotic resistance and are potential sources of multidrug resistance. High levels of antibiotic resistance have been linked to domestic animals. Our results support strengthening surveillance to monitor the emergence and effects of antibiotic-resistant microorganisms in animals.

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Section: Discussionmentioning

confidence: 72%

Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand

et al. 2022

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“…Also, 376 recovered E. coli isolates from Hooded and White-naped cranes in Japan were found resistant to oxytetracycline, ampicillin and nalidixic acid antibiotics. A high resistance level was also recorded against tetracycline followed by sulfamethoxazole, ampicillin, trimethoprim and ciprofloxacin in most E. coli isolates (Suenaga et al 2019 ). Furthermore, 87 of 88 egret’s and 53 of 55 cattle E. coli isolates were found to have MDR against more than one antimicrobial.…”

Section: Discussionmentioning

confidence: 99%

Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle

Ibrahim¹,

Salah-Eldein²,

Al-Zaban³

et al. 2023

Onderstepoort Journal of Veterinary Research

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To date, there is limited data about the genetic relationship of Escherichia coli between wild birds and cattle because these birds act as silent vectors for many zoonotic bacteria. This study aimed to elucidate the role of rooming wild birds in the vicinity of cattle farm in transmission of the same pathogenic E. coli variants, identifying their virulence, resistance traits and genetic similarities of fimH virulence gene. About 240 faecal/cloacal swabs were collected from both species and examined bacteriologically. Escherichia coli was yielded in 45.8% and 32.5%, respectively, of examined cattle and wild birds. The most prevalent detected E. coli serovar was O26. High tetracycline and chloramphenicol resistance were recorded; however, gentamycin and ciprofloxacin exhibited the highest sensitivity rates. Polymerase chain reaction (PCR) conserved genotypic resistance (tetA and blaCTX-M) and virulence attributes (fimH, stx1, eaeA and ompA) of E. coli isolates were discussed in detail. The fimH gene revealed 100% sequence similarity when comparing with different E. coli isolates globally and locally. Finally, a close genetic association of E. coli with both wild birds and cattle was detected, thus strengthening its role in the dissemination of the infection via environment. Prevention and conservative policy should be carried as E. coli constitute enormous significant zoonotic risks to livestock and animal workers. Also, further studies to the whole genome sequencing of fimH, other virulence and resistance genes of E. coli are recommended trying to limit the possibilities of co-infection and transfer among different species.Contribution: The current study recorded updated data about the critical infectious role of wild birds to livestock, including cattle farms in Egypt. It also delivered some recommendations for good hygienic practices in cattle farms which must be implemented for handling animal manure.

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“…Among these crane species, both hooded cranes and white-naped cranes are classified as vulnerable species on the International Union for Conservation of Nature (IUCN) Red List (BirdLife International., 2016. The Izumi plain has thus been targeted as an ideal location to perform a range of crane conservation activities, including infectious disease surveillance (Khalil et al, , 2021Kitadai et al, 2010Kitadai et al, , 2012Maeda et al, 2001;Nakagawa et al, 2018;Okuya et al, 2015Okuya et al, , 2017Ozawa et al, 2015Ozawa et al, , 2019Suenaga et al, 2019;Takase et al, 2020). The newly discovered CrAdV-1 is a cause of concern as a potential pathogen for which only very limited information has been established.…”

Section: E2801mentioning

confidence: 99%

Prevalence and organ tropism of crane‐associated adenovirus 1 in cranes overwintering on the Izumi plain, Japan

Esaki

Ito

Tokorozaki

et al. 2022

Transbounding Emerging Dis

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Crane-associated adenovirus 1 (CrAdV-1) is a proposed novel virus in the genus Aviadenovirus, first detected in faecal samples from hooded cranes (a vulnerable crane species) on the Izumi plain, a major overwintering site for migratory cranes in Japan.CrAdV-1 was genetically characterized in that study; however, its virological characteristics remain largely unclear. To investigate the prevalence and organ tropism of CrAdV-1, we collected swab and organ samples from dead or debilitated cranes on the Izumi plain. CrAdV-1 gene was detected in 47% (45/95) of tested cranes, comprising mainly hooded cranes but also white-naped and sandhill cranes. These results indicate that CrAdV-1 shedding is widespread among cranes overwintering on the Izumi plain.Phylogenetic analyses revealed that the 68 nucleotide sequences determined from the positive swabs formed a single cluster, suggesting phylogenetic differences between CrAdV-1 and other aviadenoviruses. CrAdV-1 prevalence showed a significant linear increase with time through the overwintering period (November to February), especially among juveniles. These findings indicate that CrAdV-1 spreads mainly by transmission between juveniles progressively through the overwintering period. The CrAdV-1 gene-positive rate was significantly higher in cloacal swabs than conjunctival or tracheal swabs. Copy numbers for the partial CrAdV-1 gene sequence were markedly high in the colon samples from three of the four cranes investigated for organ tropism. We also detected relatively high copy numbers in the cerebrum, trachea, lung and heart, suggesting that CrAdV-1 mainly targets these four organs and transmitted via the faecal-oral route and airborne transmission. These results contribute to further understanding of the virological characteristics of CrAdV-1.

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Surveillance of antibiotic resistance in <i>Escherichia coli</i> isolated from wild cranes on the Izumi plain in Kagoshima prefecture, Japan

Cited by 8 publications

References 17 publications

Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand

Phylogenetic analysis and antibiotic resistance of Escherichia coli isolated from wild and domestic animals at an agricultural land interface area of Salaphra wildlife sanctuary, Thailand

Monitoring the genetic variation of some Escherichia coli strains in wild birds and cattle

Prevalence and organ tropism of crane‐associated adenovirus 1 in cranes overwintering on the Izumi plain, Japan

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