Surveillance and genetic characterization of rotavirus strains circulating in four states of North Indian children

Islamuddin, Mohammad; Khan, Wajihul Hasan; Gupta, Shipra; Tiku, Vasundhara Razdhan; Khan, Naushad; Akdag, Ali Ilter; Chaudhary, Sanjeev; Upadhyay, Amit; Kumar, Praveen; Ghatwala, Geeta; Ray, Pratima

doi:10.1016/j.meegid.2018.04.030

Cited by 6 publications

(11 citation statements)

References 49 publications

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“…40 In a 3-year study (2013)(2014)(2015)(2016) in HP, we found the predominance of P-genotype P [6] pairing with G12. 18,19 Interestingly, the higher percentage of P [6] in HP has been a history. 43 Further, the prevalence of particular rotavirus genotype in a region depends on various factors including histo-blood group antigens (HBGAs) of the population or individuals.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, it becomes essential to study the characteristics of circulating rotavirus strains in this state. We earlier reported the epidemiology and genetic diversity of RVA in diarrheic children in HP from 2013 to 2016 (prevaccination era) 18,19 . This surveillance study identified seven G‐genotypes (G1, G2, G3, G4, G9, G10, and G12) and four P‐genotypes (P[6], P[4], P[8], and P[11]) occurring in diverse G/P combinations 18,19 .…”

Section: Introductionmentioning

confidence: 99%

“…We documented the overall detection of 17 different rotavirus strains from this region including both common rotavirus strains (G12P[6], G1P[8], G2P[4], G1P[6], and G3P[8]) and unusual rotavirus strains (G9P[4], G2P[6], G2P[8], G12P[4], and G1P[11]). The regionally common rotavirus strains G3P[6], G4P[6], G9P[6], G9P[8], G10P[6], G10P[8], and G12P[8] were observed at very low frequency 18,19 . Of note, G12 was the most predominant genotype circulating in HP during 2013–2016 18,19 .…”

Section: Introductionmentioning

confidence: 99%

“…The regionally common rotavirus strains G3P[6], G4P[6], G9P[6], G9P[8], G10P[6], G10P[8], and G12P[8] were observed at very low frequency 18,19 . Of note, G12 was the most predominant genotype circulating in HP during 2013–2016 18,19 . There is no data available about the evolutionary and antigenic characteristics for G12 rotavirus strains prominent in this region.…”

Section: Introductionmentioning

confidence: 99%

“…The present study reports the genetic characterization of VP7 and VP4 encoding genes of HP G12 strains to understand their origin and evolution. We also compared the clinical characteristics of G12 RVA with other rotavirus strains (referred as non‐G12 RVA) detected in our previous study from HP 18,19 . This prevaccine data will be helpful in assessing the impact of vaccine introduction on the rotavirus strains which are not included in any rotavirus vaccine(s) and vice versa.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Gupta

Gauhar

Bubber

et al. 2021

Journal of Medical Virology

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G12 strains are now considered to be the sixth most prevalent human rotaviruses globally. India has introduced rotavirus vaccine Rotavac® into the national immunization program in 2016 and Himachal Pradesh (HP) is the first state to launch it. During epidemiological rotavirus surveillance in HP, predominance of G12 rotaviruses was observed. This study investigated the genetic variability and evolution of HP G12 strains (n = 15) associated with P-genotypes P[6], P[4], and P[8] identified between 2013 and 2016. Phylogenetic analysis of VP7 gene revealed that all characterized G12 strains clustered in lineage-III and diversified into three subclusters indicating that these strains may have originated from three different ancestral G12 strains. The comparative sequence analysis of HP strains with Rotavac® and Rotarix® vaccine strains revealed various amino acid substitutions in epitope regions of VP7 and VP4 proteins especially at the antibody neutralization sites. Only 12/29 VP7 epitope residues and 2/25 VP4 epitope residues were found to be conserved between HP rotavirus strains and vaccine strains. Both long and short electropherotypes were observed in G12P[4] strains, while a single long electropherotype was observed in G12P[6] strains. Children of ≤11 months were significantly infected with G12 rotaviruses. The frequency of vomiting episodes (≥5/ day) was significantly higher in children infected with G12 rotavirus strains as compared to non-G12 rotaviruses (p = 0.0405). Our study provides the comprehensive data on clinical characteristics and evolutionary pattern of the G12 rotavirus, the most prevalent strain in HP and emphasizes the need to monitor these strains for inclusion in future vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Gupta

Gauhar

Bubber

et al. 2021

Journal of Medical Virology

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Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India

Akdag

Gupta

Khan

et al. 2019

Journal of Medical Virology

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There are limited reports on the etiology of multiple enteric viruses causing acute gastroenteritis (AGE) in North India. In the present study we have determined the prevalence of three enteric viruses, namely rotavirus, astrovirus (AstV) and adenovirus (AdV) in a total of 312 diarrheic children (<5 years) hospitalized at Lala Lajpat Rai Memorial Medical College, Meerut, Uttar Pradesh from August 2014 to July 2016; and results were compared with data from Delhi. The fecal samples were individually screened for group A rotavirus (RVA), AdV, and AstV using enzyme immunoassay kits. At least one viral agent was detected in 29.2% of 312 fecal specimens. RNA of rotavirus antigen‐positive samples was extracted by TRIzol method. Rotavirus G/P genotyping was performed using seminested multiplex reverse transcriptase‐polymerase chain reaction. RVA was the most predominant virus (18.3%) followed by AstV (12.5%), and AdV (9.9%). Coinfections were detected in 10.6% cases and the most common coinfection in diarrheic children was RVA combined with AstV (36.4%). Overall, the enteric viruses were found most prevalent in the 6 to 11 months age group (P = .01). Increased duration of vomiting (≥3 days) was significantly (P = .04) associated with AdV infection (61.3%) as compared with AstV (30.76%) and rotavirus (26.31%). G1P[8] was detected throughout as the most prevalent rotavirus strain (10.5%). Unusual RV strains like G2P[6] and G2P[8] were also detected. Of note G3, G4, and G12 rotavirus were detected for the first time in Meerut. This is the first report that demonstrated the important contribution of multiple enteric viruses causing AGE in young children in this part of Uttar Pradesh (Meerut).

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Epidemiology of Acute Gastroenteritis Caused by Rotavirus among Children Less than Five Years Old Admitted in Hospital, in North India

Gupta

Bansal

et al. 2021

Indian J Pediatr

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Surveillance and genetic characterization of rotavirus strains circulating in four states of North Indian children

Cited by 6 publications

References 49 publications

Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Phylogenetic analysis of VP7 and VP4 genes of the most predominant human group A rotavirus G12 identified in children with acute gastroenteritis in Himachal Pradesh, India during 2013–2016

Epidemiology and clinical features of rotavirus, adenovirus, and astrovirus infections and coinfections in children with acute gastroenteritis prior to rotavirus vaccine introduction in Meerut, North India

Epidemiology of Acute Gastroenteritis Caused by Rotavirus among Children Less than Five Years Old Admitted in Hospital, in North India

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