Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology

Yoshinaga, Túlio Teruo; Katayama, Naoto; Hattori-Ihara, Shoko; Tsukamoto, Rosângela; Takahashi, Neuza Sumico; Tabata, Yara Aiko

doi:10.1016/j.aquaculture.2019.03.037

Cited by 27 publications

(18 citation statements)

References 42 publications

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“…In previous studies, fish GCT within family level had been reported in many species, including transplanting Niber croaker spermatogonia into allogeneic larvae (intra-species, Yoshikawa et al, 2016 ), Pejerrey ( O. bonariensis ) spermatogonia into adult patagonian pejerrey ( O. hatcheri ) (intra-genus, Majhi et al, 2014 ), and Atlantic salmon ( S. salar ) spermatogonial cells into rainbow trout larvae (inter-genus, Hattori et al, 2019 ). And donor-derived gametes and offspring were successfully produced.…”

Section: Discussionmentioning

confidence: 99%

“…The histology results showed the turbot spermatogenesis conformed to the recipients during the process of testis development and maturation. In inter-family transplantation between Japanese flounder and turbot, the Japanese flounder still could surrogate the turbot for gametes production, although the donor and recipient required different water temperature and photoperiod control to regulate gonad maturation, even their spermatogenesis belonged totally two different spermatogenesis types, suggesting that the mechanisms underlying donor germ cells migration, incorporation and maturation in recipients were conserved across a wide range of teleost species ( Takeuchi et al, 2004; Saito et al, 2008; Silva et al, 2016; Hattori et al, 2019 ). Moreover, the turbot cells were transplanted at hatched Japanese flounder larvae stage, and remained in the recipients for a longer period (more than 2 years) compared with previous distant transplantations ( Saito et al, 2008; Higuchi et al, 2011; Bar et al, 2015; Silva et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

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Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish

Zhou

Liu²,

Yang³

et al. 2021

Preprint

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As a promising biotechnology, fish germ cell transplantation shows potentials in conservation germplasm resource, propagation of elite species, and generation of transgenic individuals. In this study, we successfully transplanted the Japanese flounder (P. olivaceus), summer flounder (P. dentatus), and turbot (S. maximus) spermatogonia into triploid Japanese flounder larvae, and achieved high transplantation efficiency of 100%, 75-95% and 33-50% by fluorescence tracking and molecular analysis, respectively. Eventually, donor-derived spermatozoa produced offspring by artificial insemination. We only found male and intersex chimeras in inter-family transplantations, while male and female chimeras in both intra-species and intra-genus transplantations. Moreover, the intersex chimeras could mature and produce turbot functional spermatozoa. We firstly realized inter-family transplantation in marine fish species. These results demonstrated successful spermatogonial stem cells transplantation within Pleuronectiformes, suggesting the germ cells migration, incorporation and maturation within order were conserved across a wide range of teleost species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish

Zhou

Liu²,

Yang³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Intraspeci c transplantation is challenging with respect to production of oocytes in good quality and su cient amount [11,36] especially in more distant species [17]. Evolutionary distance between gold sh and carp is about 34 mya.…”

Section: Discussionmentioning

confidence: 99%

“…The greatest phylogenetic divergence in term of successful production of donor-derived eggs has been achieved between Atlantic salmon and rainbow trout which diverged 46 mya but obtained eggs did not give rise to viable progeny [17]. Two gold sh chimeric females produced thousands of oocytes with fertilization rates over 70% which could be regarded as very acceptable.…”

Section: Discussionmentioning

confidence: 99%

“…Monosex stocks can be produced from donors with homogametic sex chromosomes [12] without the need to use challenging techniques of uniparental inheritance induction or hormonal treatment having environmental concerns [13]. Interspeci c surrogacy is also believed to be a convenient tool for the management of endangered [14][15][16] or important aquaculture species [17][18][19][20]. In both cases, interspeci c transplantation can ameliorate unfavourable characteristics of target species such as long maturation, big body size and consequently high space and costs requirements associated with their maintenance [21] or in overall problematic reproduction in captivity [22,23].…”

Section: Introductionmentioning

confidence: 99%

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Production of common carp donor-derived offspring from goldfish surrogate broodstock

Franěk

Kašpar

Gela

et al. 2020

Preprint

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Background: Common carp is the fourth most-produced species in worldwide aquaculture. Significant efforts are invested in breeding and preservation of genetic integrity of this important species. However, maintaining carp gene bank in situ can be considered as demanding due to its big body size. Recent progress in reproductive biotechnologies in fish allows improving some unfavourable characteristics of a target species using surrogate reproduction. Germ stem cells (gamete precursors) from one species are transplanted into different surrogate species with small body size. After maturation, surrogates are producing donor-derived progeny. Efficient protocols for cryopreservation of carp male and female germ stem cells have been developed lately. Thus, the next logical goal was to assess the potential of goldfish surrogate to produce donor-derived gametes of common carp after intraperitoneal transplantation of testicular cells. Results: High transplantation success was achieved when 44% of the surviving goldfish produced pure donor-derived gametes of common carp. More importantly, both viable eggs and sperm giving rise to pure common carp progeny were produced, witnessing sustainability of the presented method. Donor-derived identity of the offspring was confirmed by genotyping and typical phenotype corresponding to the donor species. Reproductive performance of chimeras was similar to goldfish controls. Assessment of gamete characteristics showed that the size of donor-derived eggs is between control carp and goldfish eggs. Interestingly, flagellum length in donor-derived spermatozoa was comparable to common carp flagellum and significantly shorter than goldfish flagellum. Conclusions: In this study, we succeeded in the production of pure common carp progeny from surrogate goldfish recipients transplanted intraperitoneally by testicular germ cells. Here we reported production of viable eggs between most distant species up to date. Good reproductive performance of goldfish germline chimeras gives a promising prospect for further analysis about the long-term reproductive performance of surrogates, recovery of cryopreserved germ cells or production of monosex stocks. Presented technology is ready to ease needs for carp breeds preservation and their recovery using many times smaller goldfish surrogates.

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Chapter 17 Intraperitoneal Germ Cell Transplantation Technique in Marine Teleosts

Takeuchi

Yazawa

Yoshizaki

2020

Reproduction in Aquatic Animals

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Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology

Cited by 27 publications

References 42 publications

Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish

Successful spermatogonial stem cells transplantation within Pleuronectiformes: first breakthrough at inter-family level in marine fish

Production of common carp donor-derived offspring from goldfish surrogate broodstock

Chapter 17 Intraperitoneal Germ Cell Transplantation Technique in Marine Teleosts

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