Surrogate Matrix And Surrogate Analyte Approaches For Definitive Quantitation of Endogenous Biomolecules

Jones, Barry R; Schultz, Gary; Eckstein, James A.; Ackermann, Bradley L.

doi:10.4155/bio.12.200

Cited by 159 publications

(74 citation statements)

References 12 publications

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“…Various approaches ( Figure 2) are followed to address the lack of blank matrices for the quantification of endogenous compounds by LC-MS/MS including, background subtraction [26-31], the standard addition method [24,25,[32][33][34][35][36][37][38][39][40][41][42][43][44], neat solutions [45][46][47][48][49][50][51][52], artificial matrices of biological fluids [23,, stripped matrices [8,10,11,[74][75][76][77][78][79][80][81][82][83][84][85][86][87][88][89][90][91], and surrogate analytes [66,[92][93][94][95][96][97]…”

Section: Approaches For Quantification Of Endogenous Analytesmentioning

confidence: 99%

Quantitative analysis of endogenous compounds

Thakare

Chhonker

Gautam

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

179

107

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Section: Approaches For Quantification Of Endogenous Analytesmentioning

confidence: 99%

Quantitative analysis of endogenous compounds

Thakare

Chhonker

Gautam

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

179

107

View full text Add to dashboard Cite

“…Researchers should consider the cost of labelled analytes, the performance of a mass spectrometer, and the type of matrix comprehensively. A comparison of surrogate matrix and surrogate analyte methods for quantitation of endogenous biomolecules concludes that both assays are well within tolerances prescribed by regulatory guidance for validation, the surrogate analyte approach allows for facile method development, and the surrogate matrix method has the long-term advantage of simplified sample analysis [15]. Another comparison shows that the surrogate analyte in authentic matrix approach performed as well as the authentic analyte in surrogate matrix approach, indicating that the surrogate analyte approach is not required for the accurate quantification of endogenous compounds in complex samples [19].…”

Section: Comparison Of the Four Methodsmentioning

confidence: 89%

Development, validation, and comparison of four methods to simultaneously quantify l-arginine, citrulline, and ornithine in human plasma using hydrophilic interaction liquid chromatography and electrospray tandem mass spectrometry

Lai

Kline

Wang

2015

Journal of Chromatography B

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M. (2015). Development, validation, and comparison of four methods to simultaneously quantify l-arginine, citrulline, and ornithine in human plasma using hydrophilic interaction liquid chromatography and electrospray tandem mass spectrometry. ABSTRACT:To understand the role of L-arginine depletion in impaired nitric oxide synthesis in disease, it is important to simultaneously quantify arginine, citrulline, and ornithine in the plasma. Because the three amino acids are endogenous analytes, true blank matrix for them is not available. It is necessary and valuable to compare the performance of different approaches due to lack of regulatory clarity for validation. A two-step sample preparation method using methanol as protein precipitation reagent was developed in this study is used for sample preparation. Because true blank matrix for endogenous analytes is not available, water as blank matrix, 1% BSA in PBS as blank matrix, surrogate analyte, and background subtraction were designed to establish successful quantification methods. Four methods to simultaneously quantify arginine, citrulline, and ornithine in human plasma using hydrophilic interaction liquid chromatography and electrospray tandem mass spectrometry were developed, validated, and compared. The developed two-step sample preparation method using methanol as protein precipitation reagent in this study needs less time and provides higher recovery comparing with other approaches. Three of the four methods, water as blank matrix, 1% BSA in PBS as blank matrix, and surrogate analyte, have been successful in fulfilling all the criteria, while background subtraction has failed. Results of the measured concentrations in 97 human plasma samples using the three methods show that the difference between any two methods or among the three methods presents 100% of samples with less than 20% for all the three amino acids and majority of them are under 10%. The developed two-step sample preparation method using methanol as protein precipitation reagent is simple and convenient. Three of the four methods are fully validated and the validation is successful. The BSA functioned effectively as a blank matrix for these three amino acids, considering cost, data quality, matrix similarity, and practicality.

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“…The use of surrogate matrix requires that a parallelism assessment is completed by spiking analyte in both surrogate matrix and authentic matrix across the assay range. If the surrogate matrix curve is 'parallel' to the authentic matrix curve then the s urrogate matrix is representative of the authentic matrix [8].…”

Section: Measuring Biomarkers In the Presence Of Endogenous Analytementioning

confidence: 99%