Abstract:3There are many enzymes that are relevant for making rare and valuable chemicals that 4 while active, are severely limited by thermodynamic, kinetic, or stability issues (e.g. 5 isomerases, lyases, transglycosidase etc.). In this work, we study an enzymatic reaction 6 system − Lactobacillus sakei L-arabinose isomerase (LsLAI) for D-galactose to D-tagatose 7 isomerizationthat is limited by all three reaction parameters. The enzyme has a low 8 catalytic efficiency for non-natural substrate galactose, has low … Show more
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