Surgically produced congenital diaphragmatic hernia in fetal rabbits

Fauza, Dario O.; Tannuri, Uenis; Ayoub, A.A.R.; Capelozzi, Vera Luíza; Saldiva, Paulo Hilário Nascimento; Maksoud, João Gilberto

doi:10.1016/0022-3468(94)90008-6

Cited by 54 publications

(29 citation statements)

References 10 publications

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“…The procedure was performed as described by Fauza, et al at 25 d of gestation (18). An incision on the uterine wall was made and the fetus was exposed.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

Varisco

Sbragia

Chen

et al. 2016

Mol Med

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“…The procedure was performed as described by Fauza, et al at 25 d of gestation (18). An incision on the uterine wall was made and the fetus was exposed.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

Varisco

Sbragia

Chen

et al. 2016

Mol Med

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“…Embora estes números não permitam avaliar se esta diferença seria estatísticamente significativa, nosso estudo é o primeiro a oferecer uma técnica alternativa válida para a produção do defeito, possivelmente mais segura que as técnicas anteriormente descritas. Quando comparamos nossos dados com estes outros estudos, devemos levar em conta a idade gestacional em que foi realizada a cirurgia fetal, pois esperamos observar uma maior mortalidade quanto menor for a idade gestacional na realização do procedimento 13 . Ambos os estudos 11,12 tiveram o procedimento realizado entre 22-23, quase na mesma idade gestacional em que os nossos casos foram operados (23 dias).…”

Section: Discussionunclassified

Estudo de uma nova técnica para criação cirúrgica de um defeito semelhante a meningomielocele em fetos de coelhos

et al. 2003

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OBJETIVO: Avaliar a sobrevida fetal usando uma nova técnica cirúrgica para criação de um defeito semelhante a meningomielocele em fetos de coelho. MÉTODOS: Foram utilizadas seis coelhas da linhagem Nova Zelândia que tiveram um defeito espinhal criado em seus fetos no 23° dia de gestação. O defeito foi criado em 19 fetos e a tentativa de correção foi feita em 15 casos (grupo I), 4 fetos permaneceram sem correção (grupo II). No 30° dia de gestação, os fetos foram submetidos a avaliação anátomo-patológica. RESULTADOS: No 30° dia a meningomielocele estava presente em todos os fetos sobreviventes. A sobrevida total foi de 73,68% (14/19), 11 fetos sobreviveram no grupo I, e 3 no grupo II. CONCLUSÃO: A técnica modificada apresenta sobrevida fetal satisfatória, no modelo experimental de criação de defeito espinhal em fetos.

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“…The rabbit is an inexpensive, widely available animal with limited housing demands and short gestational duration. A surgical CDH rabbit model has been described inducing the morphological lung changes observed in the human conditions both in the airways and in the vasculature (Fauza et al, 1994;Wu et al, 2000;Roubliova et al, 2004). However the molecular retinoic pathway has been poorly studied in the lung parenchymal tissue of this specie.…”

Section: Introductionmentioning

confidence: 99%

Expression of retinoid receptors during rabbit lung development

Coste

Gallot²,

Marceau³

et al. 2007

Animal

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Rabbit lung is often used to study the pathophysiology of some congenital anomalies affecting the lung because its development is very comparable with that of human. Retinoids and the molecular transducers of the retinoic signal play a crucial role in mammalian lung development. In the rabbit, the molecular retinoic pathway has so far been poorly studied. As a first step in elucidating this process, we aimed to identify the retinoic acid receptors (RARs) and retinoid X receptors (RXRs). We cloned a part of the nuclear receptors (RARs and RXRs), and we used reverse transcription -polymerase chain reaction (RT-PCR) and immunohistochemistry assays to demonstrate the presence of RAR (a,b) and RXR (a, b) at all stages of rabbit lung development. Our results initiate further analysis into the molecular and genetic functions of retinoids during normal and pathological rabbit lung development including the surgical model of congenital diaphragmatic hernia.Keywords: lung development, rabbits, retinoids, retinoic acid receptor, retinoid X receptor IntroductionMany factors including hormonal, biochemical and physical factors have been identified that modulate growth and development of the lung (Keijzer et al., 2000;Cardoso, 2001;Copland and Post, 2004;Rajatapiti et al., 2005). Among the variety of biochemical agents identified so far, retinoids (active derivatives of vitamin A) play a clearly defined role in lung development at its different stages (Gallot et al., 2005;Maden, 2004). Vitamin A (retinol) exerts important effects upon a vast number of development processes via signalling through heterodimerization complexes comprising retinoic acid receptors (RARs) and retinoid X receptors (RXRs) (Chambon, 1996). These receptors belong to the steroid/thyroid hormone receptor super family of ligand-inducible transcription regulators known to control the expression of target genes. All members of this family share a strongly conserved modular structure with discrete functional domains for ligand binding, DNA binding and transactivation. There are three RAR isotypes (RARa, b, g) activated by both all-trans and 9-cis retinoic acid (RA) and three retinoid X receptors (RXRa, b, g) only activated by 9-cis RA (Bastien and Rochette-Egly, 2004).The RAR and RXR proteins interact with each other to form RAR/RXR heterodimers which are able to bind to specific DNA sequences called RA responsive elements usually located within the 5 0 -regulatory region of retinoid-regulated genes.The importance of vitamin A in vertebrate lung development was first demonstrated by the observation that foetuses from pregnant rats which were fed a vitamin Adeficient diet had either agenesis of the left lung or rudimentary lung buds (Warkany and Wilson, 1948;Chazaud et al., 2003). Double mutant mice RARa, b 2/2 revealed defects comparable with the consequences of vitamin A deficiency including hypoplastic lungs (Mendelsohn et al., 1994;Kastner et al., 1997;Mascrez et al., 1998). Abnormalities in the vitamin A status and/or metabolism may also play a role in t...

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Surgically produced congenital diaphragmatic hernia in fetal rabbits

Cited by 54 publications

References 10 publications

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

Excessive Reversal of Epidermal Growth Factor Receptor and Ephrin Signaling Following Tracheal Occlusion in Rabbit Model of congenital Diaphragmatic Hernia

Estudo de uma nova técnica para criação cirúrgica de um defeito semelhante a meningomielocele em fetos de coelhos

Expression of retinoid receptors during rabbit lung development

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