Surgical Trauma Leads to a Shorter Survival in a Murine Orthotopic Pancreatic Cancer Model

Menges, Pia; Klöcker, Christian; Diedrich, Stephan; Sendler, Matthias; Maier, Stefan A.; Weiss, Frank Ulrich; Heidecke, Claus–Dieter; Bernstorff, Wolfram von; Partecke, Lars Ivo

doi:10.1159/000368226

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…In the present study, SII had no impact on the overall survival. This is in contrast to the results reported by Menges et al [ 24 ]. In this study, tumour-bearing animals subjected to SID showed a significantly reduced survival compared to the non-operated control mice, while tumour growth was similar in both experimental groups.…”

Section: Discussioncontrasting

confidence: 99%

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Visceral Surgery Profoundly Affects the Cellular and Humoral Components of the Anti-Tumour Immune Response in a Murine Pancreatic Adenocarcinoma Model

Loening

Kleinwort

Partecke

et al. 2022

Cancers

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(1) Background: Surgery is the most important element of multimodal treatment concepts in oncological patients, especially in the early stages of pancreatic tumours. While the influence of primary tumour resection on the immune status was analysed in several studies, the impact of tumour-unrelated visceral surgery on the tumour-bearing organism and on the primary tumour itself is not yet fully understood. (2) Methods: We combined a murine model of orthotopically implanted adenocarcinoma of the pancreas with the model of surgically-induced immune dysfunction (SID). Mortality and general condition including body weight were observed over a period of 28 days. Tumour growth was analysed by MRI scans on days 8 and 27 following tumour implantation. On day 28, the immune cell populations in the blood and spleen as well as the serum cytokines were quantified. (3) Results: SID results in a significant deterioration of the general condition and a reduced increase in the body weight of tumour-bearing mice compared to the control groups, while mortality and tumour growth rate were not influenced. The numbers of spleen macrophages and neutrophils were increased in tumour-bearing animals following SID. Furthermore, both macrophage and neutrophil levels were increased in the peripheral blood. (4) Conclusions: The presented results might contribute to the basic understanding of the interaction of tumour and immune system and could contribute to new approaches to immunotherapeutic strategies.

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Section: Discussioncontrasting

confidence: 99%

“…In accordance with previous studies, we observed no influence of SID on the tumour growth as analysed by MRI scan on day 8 and day 27 following the tumour implantation [ 24 ]. The increase in tumour volume is only one aspect of the disease progress, while the interaction of immune response and tumour progress is most complex.…”

Section: Discussionsupporting

confidence: 93%

Visceral Surgery Profoundly Affects the Cellular and Humoral Components of the Anti-Tumour Immune Response in a Murine Pancreatic Adenocarcinoma Model

Loening

Kleinwort

Partecke

et al. 2022

Cancers

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“…MRI plays an important role in the molecular and functional imaging of cancer [12]. Our group has published interesting results for noninvasive monitoring of tumor growth in a murine model for pancreatic cancer [13] and for tumor growth of pancreatic cancer following surgical trauma [14]. In inflammatory situations MRI can generate morphologic information at the site of infection by providing three-dimensional spatially resolved information.…”

Section: Discussionmentioning

confidence: 99%

The MRI Sepsis Score: An Innovative Tool for the Evaluation of Septic Peritonitis in Mice Using 7-Tesla Small Animal MRI

et al. 2018

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Background: Magnetic resonance imaging (MRI) techniques are rarely used in the context of abdominal sepsis and in sepsis research. This study investigates the impact of MRI for monitoring septic peritonitis in an animal model (colon ascendens stent-induced peritonitis, CASP). The CASP model closely mimics that of human disease and is highly standardized. The most frequently employed readout parameter in mouse CASP studies is prolonged or decreased rate of survival. Monitoring the progression of peritonitis via MRI could provide a helpful tool in the evaluation of severity. The use of alternative readout systems could very well reduce the number of research animals. Perspectively, clinical improvement after certain treatment could be classified. Methods: This study describes for the first time MRI findings following the induction of septic peritonitis in mice using the CASP model. Two sublethal groups of mice with septic peritonitis were investigated. Each had received one of two differing stent diameters in order to control the leakage of feces into the abdominal cavity. Each mouse served as its own control. Imaging and analyses were performed blinded. Gut diameters, stomach volume, abdominal organ wall diameters, and volume of the adrenal glands were measured. Serum corticosterone levels were detected using ELISA. Serum IL-6, TNF-α, IL-1β, and IL-10 levels were screened by cytometric bead array. Statistical analysis was performed using the Mann-Whitney U test for nonparametric probes and the Kruskal-Wallis and t tests. Results: Using a 7-tesla MRI scanner 24 and 48 h after induction of septic peritonitis, interenteric fluid, organ swelling of spleen and adrenal glands, as well as dilatation of the stomach were compared to nonseptic conditions. Swelling of adrenal glands resulted in an increased serum corticosterone level. In addition, the wall of the intestine bowel was thickened. Based upon these findings, an MRI score (MRI sepsis score, MSS) for abdominal sepsis in mice was established. Reduced stent sizes led to reduced severity of the abdominal sepsis, which could be reproduced in the MSS, which is described here for the first time. Conclusions: Intraabdominal variations during septic peritonitis are detectable by MRI techniques. MRI methods should become a more important tool for the evaluation of abdominal peritonitis. MSS could provide an interesting tool for the evaluation of therapeutic strategies.

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Adjuvant or Neoadjuvant Therapy in the Treatment in Pancreatic Malignancies

Wolff¹

2018

Surgical Clinics of North America

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Surgical Trauma Leads to a Shorter Survival in a Murine Orthotopic Pancreatic Cancer Model

Cited by 3 publications

References 21 publications

Visceral Surgery Profoundly Affects the Cellular and Humoral Components of the Anti-Tumour Immune Response in a Murine Pancreatic Adenocarcinoma Model

Visceral Surgery Profoundly Affects the Cellular and Humoral Components of the Anti-Tumour Immune Response in a Murine Pancreatic Adenocarcinoma Model

The MRI Sepsis Score: An Innovative Tool for the Evaluation of Septic Peritonitis in Mice Using 7-Tesla Small Animal MRI

Adjuvant or Neoadjuvant Therapy in the Treatment in Pancreatic Malignancies

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