Surgical Therapy of Cutaneous Melanoma

Blazer, Dan G.; Sondak, Vernon K.; Sabel, Michael S.

doi:10.1053/j.seminoncol.2007.03.007

Cited by 13 publications

(3 citation statements)

References 66 publications

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“…The detection and eradication of nodal micrometastases is not only associated with improved outcome, but also helps select patients for adjuvant systemic therapy 3. SLN biopsy is recommended for patients with melanomas 1.0mm or thicker, or for patients with melanoma <1.0mm but with other adverse features such as ulceration, high mitotic rate or angiolymphatic invasion 4. Using this criteria, regional micrometastases are identified in approximately 20 to 25% of patients, exposing a large number of patients to the cost and morbidity of the procedure to benefit a small fraction.…”

Section: Introductionmentioning

confidence: 99%

“…3 SLN biopsy is recommended for patients with melanoma 1.0 mm or thicker, or for patients with melanoma <1.0 mm but with other adverse features such as ulceration, high mitotic rate, or angiolymphatic invasion. 4 Using this criteria, regional micrometastases are identified in approximately 20 to 25% of patients, exposing a large number of patients to the cost and morbidity of the procedure to benefit a small fraction. As with SLN biopsy, adjuvant therapy is offered to a large number of patients even though benefit is realized in only a few.…”

Section: Introductionmentioning

confidence: 99%

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Serum Autoantibody Profiling Using a Natural Glycoprotein Microarray for the Prognosis of Early Melanoma

Liu

Xie

et al. 2010

J. Proteome Res.

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The poor prognosis of melanoma and the high-cost of lymph node biopsy for melanoma patients have led to an urgent need for the discovery of convenient and accurate prognostic indicators. Here, we have developed a natural glycoprotein microarray to discover serum autoantibodies to distinguish between patients with node negative melanoma and node positive melanoma. Duallectin affinity chromatography was used to extract glycoproteins from a melanoma cell line. Liquid-based reverse phase separation and microarray platforms were then applied to separate and spot these natural proteins on nitrocellulose slides. The serum autoantibodies were investigated by exposing these proteins to sera from 43 patients which have already been diagnosed to have different stages of early melanoma. The combination of 9 fractions provides a 55% sensitivity with 100% specificity for the detection of node positive against node negative and a 62% sensitivity with 100% specificity for the detection of node negative against node positive. Recombinant proteins were used to confirm the results using a sample set with 79 patients with diagnosed melanoma. The response of sera against recombinant 94 kD glucose-regulated protein (GRP94), acid ceramidase (ASAH1), cathepsin D (CTSD), and lactate dehydrogenase B (LDHB) shared a similar pattern to the fractions where they were identified. The glycoarray platform provides a convenient and highly-reproducible method to profile autoantibodies which could be used as serum biomarkers for prognosis of melanoma.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Serum Autoantibody Profiling Using a Natural Glycoprotein Microarray for the Prognosis of Early Melanoma

Liu

Xie

et al. 2010

J. Proteome Res.

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“…Clinical therapy for melanoma is based mainly on surgery and radiation therapy (10). However, most of these therapies exert limited effects and act only in the early stage of melanoma.…”

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confidence: 99%

Antiangiogenic Tumor Therapy by DNA Vaccine Inducing Aquaporin-1–Specific CTL Based on Ubiquitin–Proteasome System in Mice

Chou

Hiromatsu

Okano

et al. 2012

The Journal of Immunology

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Aquaporin-1 (AQP-1) is a water channel protein highly expressed in the vascular endothelial cells of proliferating tissues including malignant cancers. Given that in APC ubiquitinated peptides are effectively introduced into proteasomes from which CD8 epitopes are excised, we fused ubiquitin with AQP-1 (pUB–AQP-1) to produce a DNA vaccine. In C57BL/6J mice immunized with pUB–AQP-1, the growth of B16F10 melanoma was profoundly inhibited. The antitumor effect of the pUB–AQP-1 DNA vaccine was largely mediated by CD8 T cells, which secrete IFN-γ, perforin, and granzyme-B in the presence of APCs transfected with pUB–AQP-1. AQP-1–specific CD8 T cells possessed cytotoxic activity both in vivo and in vitro. After tumor challenge, the microvessel density decreased and the ratio of total blood vessel area to tumor area was significantly reduced as compared with control mice, resulting in a dramatic suppression of tumor growth. The immunization effect was completely abrogated in immunoproteasome-deficient mice. Strikingly this pUB–AQP-1 DNA vaccine was also effective against Colon 26 colon tumors (BALB/c) and MBT/2 bladder tumors (C3H/HeN). Thus, this ubiquitin-conjugated DNA immunization-targeting tumor vasculature is a valid and promising antitumor therapy. This vaccine works across the barriers of tumor species and MHC class I differences in host mice.

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Melanoma

McCarter¹

2009

Abernathy's Surgical Secrets

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Surgical Therapy of Cutaneous Melanoma

Cited by 13 publications

References 66 publications

Serum Autoantibody Profiling Using a Natural Glycoprotein Microarray for the Prognosis of Early Melanoma

Serum Autoantibody Profiling Using a Natural Glycoprotein Microarray for the Prognosis of Early Melanoma

Antiangiogenic Tumor Therapy by DNA Vaccine Inducing Aquaporin-1–Specific CTL Based on Ubiquitin–Proteasome System in Mice

Melanoma

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