Surgical stress detection in human blood plasma by DSC

Abstract

It is a well-known fact that the extension of the surgical intervention influences both the success and time of the patient's recovery, the degree of the blood loss, i.e., overall the patients' surgical burden. Disease itself determines extent of surgical procedure (minor, intermediate or major surgery), which affects the risk and frequency of complications. Previous works have contributed to the validation of differential scanning calorimetry (DSC) as a potential non-invasive tool for diagnosing and monitoring several illnesses. Hence, the main goal of this study was to measure the effect of each surgical intervention on its own to blood plasma composition. Peripheral venous blood samples were collected from patients who underwent minor (n = 8), intermediate (n = 9) and major surgical interventions (n = 7). According our DSC data of blood plasma components, from the thermodynamic parameters, namely from the thermal transitions (T m1-T m8) to calorimetric enthalpy (ΔH cal) in proportion corresponded to the size of surgical interventions (duration of operation time, length of incision, surgical intraoperative stress, blood loss, etc.). This examination has shown that intraoperative stress during any surgical intervention affects the composition of plasma proteins, which should be always considered in the evaluation of DSC results in any surgical study.

Deconvoluted DSC curves of intestinal muscle layer following warm and cold ischaemic injury

“…As described in our previous blood plasma measurements [13][14][15], the obtained DSC scans can be decomposed into a sum of Gaussian curves that way that their total area is nearly the same as of the experimental curve one, within a reasonable error (R 2 changed between 0.9507 and 0.9918).…”

“…Blood plasma analysis of oncological patients has come to the forefront [9][10][11][12]. Another forward step in research, when the use of deconvolution methods has become increasingly common with the further analysis of DSC curves [13][14][15]. A recent advance was the study in which the blood plasma proteome of colorectal cancer patients was classified, deconvoluted and subdivided based on the derived thermodynamic parameters.…”

Comparison of human blood serum DSC profiles in aqueous and PBS buffer solutions

“…Recently, it has been recognized as having potential in diagnostics and monitoring of diseases. DSC has been used to characterize thermal denaturation transitions in various biological fluids, in particular, blood plasma [1][2][3][4][5][6][7][8][9], serum [10][11][12][13][14][15][16], synovial fluid [17][18][19], cerebrospinal fluid [20,21]. This highly sensitive method allows detecting, registering and analysis the changes taking place in various diseases, during treatment, after high-intensity exercise or because of other efforts and stressors .…”

“…Dilution of serum samples in PBS is the most common practice as it provides the closest to physiological conditions. Most publications showing the diagnostic power of DSC have analyzed plasma profiles in pH 7.4 buffer solutions [1][2][3][4][5][6][7][8][9]. We also used this buffer in some of our earlier research [14,15,24].…”