Surgical outcomes of extracorporeal irradiation and re-implantation in extremities for high grade osteosarcoma: A retrospective cohort study and a systematic review of the literature

Pruksakorn, Dumnoensun; Kongthavonskul, Jatupon; Teeyakasem, Pimpisa; Phanphaisarn, Areerak; Chaiyawat, Parunya; Klangjorhor, Jeerawan; Arpornchayanon, Olarn

doi:10.1016/j.jbo.2018.100210

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Combined therapy strategies, including surgery and neoadjuvant chemotherapy, have been applied for treating OS. Nevertheless, the overall survival rate of OS patients is still low, particularly for those with distant metastasis [3] . Tumor resection or standard adjuvant chemotherapy is futile for patients tolerated with severe metastasis [4] , [5] .…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA BLACAT1 expedites osteosarcoma cell proliferation, migration and invasion via up-regulating SOX12 through miR-608

Chen

Cui²

2020

Journal of Bone Oncology

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Section: Introductionmentioning

confidence: 99%

Long non-coding RNA BLACAT1 expedites osteosarcoma cell proliferation, migration and invasion via up-regulating SOX12 through miR-608

Chen

Cui²

2020

Journal of Bone Oncology

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“…Described by Spira and Lubin in 1968 [ 87 ], this technique consists of extracorporeal irradiation with reimplantation of a tumour-bearing bone [ 88 , 89 ]. The first step is to remove the tumour mass with an appropriate margin, along with its surrounding soft tissues [ 4 , 90 ].…”

Section: Reconstruction Using Biological Materialsmentioning

confidence: 99%

“…Tendons and ligaments are preserved to be reused after irradiation [ 8 , 89 ]. Irradiation consists of 60–70 Gy in a single dose [ 88 , 90 ]. A limitation of this procedure is that the irradiated bone fragments have no blood supply and this can lead to infections, fractures, non-union, and bone re-absorption [ 90 , 91 ].…”

Section: Reconstruction Using Biological Materialsmentioning

confidence: 99%

Strategies for large bone defect reconstruction after trauma, infections or tumour excision: a comprehensive review of the literature

et al. 2021

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Large bone defects resulting from musculoskeletal tumours, infections, or trauma are often unable to heal spontaneously. The challenge for surgeons is to avoid amputation, and provide the best functional outcomes. Allograft, vascularized fibular or iliac graft, hybrid graft, extracorporeal devitalized autograft, distraction osteogenesis, induced-membrane technique, and segmental prostheses are the most common surgical strategies to manage large bone defects. Given its optimal osteogenesis, osteoinduction, osteoconduction, and histocompatibility properties, along with the lower the risk of immunological rejection, autologous graft represents the most common used strategy for reconstruction of bone defects. However, the choice of the best surgical technique is still debated, and no consensus has been reached. The present study investigated the current reconstructive strategies for large bone defect after trauma, infections, or tumour excision, discussed advantages and disadvantages of each technique, debated available techniques and materials, and evaluated complications and new perspectives.

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“…Extra corporeal irradiation (ECIR) treated samples preparation. A total of 12 OGS samples were subjected to ECIR treatment at 15,000 gamma irradiations 51 . After irradiation the samples were subjected to protein extraction using RIPA lysis buffer (50 mM Tris-HCl pH7.2, 150 Mm NaCl,1% NP40, 0.1% SDS, 0.5% DOC, 1 mM PMSF, 25 mM MgCl2 (sigma; R0278)) supplemented with a phosphatase inhibitor cocktail (Thermo; 78420).…”

Section: Protein Extraction From Ogs Samples Cryotherapy Treated Sammentioning

confidence: 99%

Proteomic profiling and identification of significant markers from high-grade osteosarcoma after cryotherapy and irradiation

Madda

Chen

Wang

et al. 2020

Sci Rep

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Biological reconstruction of allografts and recycled autografts have been widely implemented in highgrade osteogenic sarcoma. For treating tumor-bearing autografts, extracorporeal irradiation (ECIR) and liquid nitrogen (LN) freezing techniques are being used worldwide as a gold standard treatment procedure. Both the methods aim to eradicate the tumor cells from the local recurrence and restore the limb function. Therefore, it is essential and crucial to find, and compare the alterations at molecular and physiological levels of the treated and untreated OGS recycled autografts to obtain valuable clinical information for better clinical practice. Thus, we aimed to investigate the significantly expressed altered proteins from ECIR-and cryotherapy/freezing-treated OGS (n = 12) were compared to untreated OGS (n = 12) samples using LC-ESI-MS/MS analysis, and the selected proteins from this protein panel were verified using immunoblot analysis. From our comparative proteomic analysis identified a total of 131 differentially expressed proteins (DEPs) from OGS. Among these, 91 proteins were up-regulated (2.5 to 3.5-folds), and 40 proteins were down-regulated (0.2 to 0.5 folds) (p < 0.01 and 0.05). The functional enrichment analysis revealed that the identified DEPs have belonged to more than 10 different protein categories include cytoskeletal, extracellular matrix, immune, enzyme modulators, and cell signaling molecules. Among these, we have confirmed two potential candidates' expressions levels such as Fibronectin and Protein S100 A4 using western blot analysis. Our proteomic study revealed that LN-freezing and ECIR treatments are effectively eradicating tumor cells, and reducing the higher expressions of DEPs at molecular levels which may help in restoring the limb functions of OGS autografts effectively. To the best of our knowledge, this is the first proteomic study that compared proteomic profiles among freezing, ECIR treated with untreated OGS in recycled autografts. Moreover, the verified proteins could be used as prognostic or diagnostic markers that reveal valuable scientific information which may open various therapeutic avenues in clinical practice to improve patient outcomes.High-grade osteogenic sarcomas (OGS) are the most common primary malignant bone sarcomas that distress the bone and forms a matrix and osteoid around the knees 1-3 . It accounts one to three per million each year worldwide and has a high rate of incidence in children and adults 3,4 . Currently, the standard treatment procedures applicable for patients are neoadjuvant chemotherapy drugs combined with surgery, precision diagnostic instruments, and limb salvage operations 5 . At present, there are three reconstructive procedures available after resection of tumors that are affected with major joints, include tumor prosthesis, an osteoarticular allograft, and a composite biological reconstruction. Among these three options, biological reconstruction of allograft and autografts (recycled from the resected autogenic bone segment) technique has ...

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Surgical outcomes of extracorporeal irradiation and re-implantation in extremities for high grade osteosarcoma: A retrospective cohort study and a systematic review of the literature

Abstract: HighlightsDiaphyseal resection of ECIR showed the promising outcome in osteosarcoma.Less complications and structural permanence are advantages of DI-ECIR.Tumor recurrence is comparable with other surgical reconstructions.

Cited by 10 publications

References 28 publications

Long non-coding RNA BLACAT1 expedites osteosarcoma cell proliferation, migration and invasion via up-regulating SOX12 through miR-608

Long non-coding RNA BLACAT1 expedites osteosarcoma cell proliferation, migration and invasion via up-regulating SOX12 through miR-608

Strategies for large bone defect reconstruction after trauma, infections or tumour excision: a comprehensive review of the literature

Proteomic profiling and identification of significant markers from high-grade osteosarcoma after cryotherapy and irradiation

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