Surgical Management of Ovarian Cancer

Schwartz, Peter E.

doi:10.1001/archsurg.1981.01380130075017

Cited by 31 publications

(10 citation statements)

References 23 publications

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“…In clinical ovarian cancer, the omentum is the primary site of metastasis formation [8,9]. The human omentum is a peritoneal fold composed of layers of mesothelial cells that envelope adipose tissue [10].…”

Section: Introductionmentioning

confidence: 99%

In vitro metastatic colonization of human ovarian cancer cells to the omentum

Khan

Funk

Thiolloy

et al. 2010

Clin Exp Metastasis

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Despite the potentially crucial contributions of the omentum in the regulation of ovarian cancer metastatic growth, it remains a poorly understood organ. Due to its anatomic location and structural fragility, the omentum presents inherent challenges to mechanism-based in vivo studies. Thus, the availability of an ex vivo omental model would, in part, address some of these difficulties posed. Here we describe a technique for identifying, isolating and maintaining ex vivo cultures of omenta from immune-compromised and -competent mice. Ex vivo culture conditions were developed that maintain tissue viability, architecture, and function for up to 10 days. Further experiments demonstrate that the ex vivo culture conditions allow for the proliferation of ovarian cancer cells in vitro and support a similar pattern of microscopic lesions after either intraperitoneal injection of ovarian cancer cells or co-culture of ovarian cancer cells with the omentum. In agreement with previous studies from our laboratory, histologic evaluation of these specimens found that ovarian cancer cells, as well as other peritoneal cancer cells, preferentially accumulate in, and colonize, omental areas rich in immune cells. We now recognize that these are specific, functional structures referred to as milky spots. In sum, these are foundational studies of a readily accessible model, which is easily manipulated and can be immediately used to study the dynamic process of omental colonization. It is hoped that investigators will use the data herein as a starting point for refinements and modifications which will enable them to tailor the model to the specific needs of the experimental question(s) they wish to pursue.

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Section: Introductionmentioning

confidence: 99%

In vitro metastatic colonization of human ovarian cancer cells to the omentum

Khan

Funk

Thiolloy

et al. 2010

Clin Exp Metastasis

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“…Improved treatment strategies require a greater and more robust understanding of the process of metastatic colonization, the final step in metastasis, in which cancer cells undergo progressive growth at secondary sites [6,7]. In clinical ovarian cancer, the omentum is the primary site of metastasis formation [8,9]. The human omentum is a peritoneal fold composed of layers of mesothelial cells that envelope adipose tissue [10].…”

Section: Discussionmentioning

confidence: 99%

“…The omentum is a highly vascularized tissue that comprises mainly adipocytes interspersed with structures known as "milky spots" which contain immune cells [5,6]. Studies from our laboratory and others have demonstrated that ovarian tumor cells preferentially seed to the milky spots suggesting that they are a favorable microenvironment for the establishment of ovarian metastases in the omentum [7,8,9]. The goal of this project is to increase our knowledge of the structure and function of milky spots in the mouse omentum, the interactions of ovarian tumor cells with these structures and identify specific cells and molecules involved in the interactions between ovarian tumor cells and the omental microenvironment.…”

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confidence: 99%

The Role of the Omental Microenvironment in Ovarian Cancer Metastatic Colonization

Rinker‐Schaeffer¹,

Krishnan²

2010

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Public reporting burden for this collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing this collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden to Department of Defense, Washington Headquarters Services, Directorate for Information Operations and Reports (0704-0188), 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to any penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. PLEASE DO NOT RETURN YOUR FORM TO THE ABOVE ADDRESS. (31-08-2010) 31-AUG-2010 REPORT DATE ABSTRACTThe omentum is a highly vascularized tissue that comprises mainly adipocytes interspersed with structures known as "milky spots" which contain immune cell. The goal of this project is to increase our knowledge of the structure and function of milky spots in the mouse omentum, the interactions of ovarian tumor cells with these structures and identify specific cells and molecules involved in the interactions between ovarian tumor cells and the omental microenvironment. SUBJECT TERMSNone provided. INTRODUCTION:The majority of ovarian cancer patients have advanced disease at the time of diagnosis. Patients undergoing surgical cytoreduction to control metastatic disease would be immediate candidates for therapies that target metastatic regrowth if they will be available. Such approaches require mechanistic knowledge of the process of metastatic colonization, the final step of metastasis in which ovarian tumor cells undergo progressive growth at the secondary sites [1,2]. Our current knowledge is limited to the fact that the omentum is the one of the most common sites of ovarian cancer metastasis [3,4] and consists of a fatty peritoneal fold that extends from the greater curvature of the stomach and covers most of the abdominal organs. The omentum is a highly vascularized tissue that comprises mainly adipocytes interspersed with structures known as "milky spots" which contain immune cells [5,6]. Studies from our laboratory and others have demonstrated that ovarian tumor cells preferentially seed to the milky spots suggesting that they are a favorable microenvironment for the establishment of ovarian metastases in the omentum [7,8,9]. The goal of this project is to increase our knowledge of the structure and function of milky spots in the mouse omentum, the interactions of ovarian tumor cells with these structures and identify specific cells and molecules involved in the interactions between ovarian tumor cells and the omental microenvironment. BODY:Aim 1: To conduct cellular and molecular characterization of omental structures and their interactions with ovarian cancer ...

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“…The forthcoming wide application of the endoscopic sur gery has made this task more urgent [ 1,2], In addition, the greatest opportunity to influence the natural course of an ovarian malignancy is obtained when accurate staging and appropriate surgery are delivered during the first operation [3][4][5], Age is an important factor correlating with the inci dence of ovarian malignancy [6], Gynecological examina tion in combination with ultrasound findings are the basic and widely available procedures of evaluating adnexal masses [7][8][9][10]. An alternative approach has been intro duced by serum CA 125 measurements [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…The above features are essential for the quality of the first operation in an ovarian malignancy and should be offered to any woman with a pelvic mass. In the present time this first operation is the best means of influencing the natural his tory of the ovarian cancer [3][4][5], Each clinician can define the upper and lower limits of the predicted probability of malignancy and modify the diagnostic and therapeutic procedures according to the individual patient and the availability of local medical facilities in a more objective way.…”

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confidence: 99%

Ovarian Tumors: Prediction of the Probability of Malignancy by Using Patient’s Age and Tumor Morphologic Features with a Logistic Model

Minaretzis

Tsionou²,

Tziortziotis³

et al. 1994

Gynecol Obstet Invest

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An attempt was made to predict the probability of malignancy of a given ovarian tumor in a certain patient by using the age and simple morphologic features of the tumor. A cohort of 959 patients with ovarian tumors was analysed retrospectively according to the patient’s age and tumor characteristics such as greatest diameter, consistency, bilaterality and diagnosis as malignant (271 patients) or benign (688 patients). All variables were entered unconditionally in a logistic regression. The presence of solid/multilocular elements has a 9.6-fold increased risk of malignancy, where a bilateral tumor has a 2.8-fold increase. Significant increase in risk of malignancy was observed in ages under 20 and over 40 years, as well as in tumors with a diameter larger than 9 cm. All variables were highly significant associated with the discrimination between benign and malignant. A formula including all variables has been developed so that the probability of malignancy can be estimated by a scientific calculator. In conclusion, simple, easily determined by ultrasound and reproducible criteria such as patient’s age, tumor size, consistency and bilaterality were assembled in a logistic model in order to predict the probability of malignancy for a given ovarian tumor, in an individual patient.

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Surgical Management of Ovarian Cancer

Cited by 31 publications

References 23 publications

In vitro metastatic colonization of human ovarian cancer cells to the omentum

In vitro metastatic colonization of human ovarian cancer cells to the omentum

The Role of the Omental Microenvironment in Ovarian Cancer Metastatic Colonization

Ovarian Tumors: Prediction of the Probability of Malignancy by Using Patient’s Age and Tumor Morphologic Features with a Logistic Model

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Surgical Management of Ovarian Cancer

Cited by 31 publications

References 23 publications

In vitro metastatic colonization of human ovarian cancer cells to the omentum

In vitro metastatic colonization of human ovarian cancer cells to the omentum

The Role of the Omental Microenvironment in Ovarian Cancer Metastatic Colonization

Ovarian Tumors: Prediction of the Probability of Malignancy by Using Patient&rsquo;s Age and Tumor Morphologic Features with a Logistic Model

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Ovarian Tumors: Prediction of the Probability of Malignancy by Using Patient’s Age and Tumor Morphologic Features with a Logistic Model