Surgical Induction of Endometriosis in Female Mice

Abstract

Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous transplantation of endometrial tissue into the abdominal compartment. Here we describe a surgical procedure to generate a syngeneic model of endometriosis in immunocompetent mice with intact uterine and ovarian tissues. In this model, four uterine fragments from a donor mouse at diestrus are sutured to the abdominal wall of a recipient mouse. One month after surgeries, endometrial implants develop into cysts with glandular epithelium and stroma, mimicking the endometriotic lesions observed in women with endometriosis. Therefore, this mouse model provides a valuable tool to study the pathophysiology of endometriosis and the efficacy of potential treatments.

Targeting the chemerin/CMKLR1 axis by small molecule antagonist α-NETA mitigates endometriosis progression

“…A syngeneic surgical model of endometriosis was established according to the protocol published online ( Escudero-Lara et al, 2020 ). Briefly, mice at diestrus were selected as the donors, and their uterine and abdominal fat tissues were excised.…”

Contribution of CD4+ cells in the emotional alterations induced by endometriosis in mice

“…Mice received subcutaneous β-estradiol (3 μg/mouse, Sigma, cat.E8875) in corn oil (Sigma, cat.C8267) 7 days before induction of endometriosis. The phase of the estrous cycle on day 0 was assessed as previously described ( Escudero-Lara et al, 2020b ). Uterine horns of donors in diestrus were excised and opened longitudinally.…”

Effect of Mirena intrauterine device combined with GNRH-A on endometriosis, sex hormone level and carbohydrate antigen 125

“…Endometriosis, as one of the common and high incidence gynecological diseases in clinics, is a local lesion caused by the appearance of endometrial tissue outside the uterine cavity, which has the behavioral characteristics of invasion, implantation and other similar to the malignant tumor. The disease is more common in women aged 20-45 years, the incidence of women of childbearing age is as high as 10%, after the onset of the disease, most of the manifestations are pain during intercourse, dysmenorrhea, infertility and other situations, causing a serious impact on women's physical and mental health, and lead to a great decline in their quality of life (1)(2)(3). The main clinical treatment methods for the disease are surgery and drugs.…”