Surgical and histopathological effects of topical Ankaferd hemostat on major arterial vessel injury related to elevated intra-arterial blood pressure

Abstract

Objective: The aim of this study was to assess the surgical and histopathological hemostatic effects of topical Ankaferd blood stopper (ABS) on major arterial vessel injury related to elevated intra-arterial blood pressure in an experimental rabbit model. Materials and Methods: The study included 14 New Zealand rabbits. ABS was used to treat femoral artery puncture on 1 side in each animal and the other untreated side served as the control. Likewise, for abdominal aortic puncture, only 50% of the aortic injuries received topical liquid ABS and the others did not (control). The experiment was performed under conditions of normal arterial blood pressure and was repeated with a 50% increase in blood pressure. Histopathological analysis was performed in all of the studied animals. Results: Mean bleeding time in the control femoral arteries was 105.0±18.3 s, versus 51.4±9.8 s (p<0.05) in those treated with ABS. Mean blood loss from the punctured control femoral arteries was 5.0±1.5 mg and 1.6±0.4 mg from those treated with ABS (p<0.05). Histopathological examination of the damaged arterial structures showed that ABS induced red blood cell aggregates. Conclusion: ABS administered to experimental major arterial vessel injury reduced both bleeding time and blood loss under conditions of normal and elevated intra-arterial blood pressure. ABS-induced erythroid aggregation was prominent at the vascular tissue level. These findings will inform the design of future experimental and clinical studies on the anti-bleeding and vascular repairing effects of the novel hemostatic agent ABS. (Turk J Hematol 2011; 28: 206-12)

Acute in Vitro effects of ABS (Ankaferd Hemostat) on the Lymphoid Neoplastic Cells (B-CLL and RAJI Tumor Cell Lines)

“…The pleiotropic effects of ABS were ascribed to the ABS-induced alterations in the pathobiological events of apoptosis [7][8][9] , angiogenesis 13,21,22 , cellular proliferation 23,24 , and cellular dynamics. 13,21 In the previous investigations, histopathological examination of the damaged vascular structures revealed ABS-induced red blood cell aggregates 1,6,25 supporting the hypothesis that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process.…”

Generation of Chimeric “ABS Nanohemostat” Complex and Comparing Its HistomorphologicalIn VivoEffects to the Traditional Ankaferd Hemostat in Controlled Experimental Partial Nephrectomy Model

“…Those pleiotropic effects were ascribed to the ABS-induced alterations in apoptosis, angiogenesis, cellular proliferation, and cellular dynamics [4–6]. In previous investigations, histopathological examination of the damaged vascular structures revealed ABS-induced red blood cell aggregates supporting the hypothesis that ABS-induced formation of the protein network with vital erythroid aggregation covers the entire physiological hemostatic process [42, 43]. We have observed the same structures in the kidney tissue in the present study.…”

Ankaferd Blood Stopper Decreases Postoperative Bleeding and Number of Transfusions in Patients Treated with Clopidogrel: A Double-blind, Placebo-controlled, Randomized Clinical Trial

“…Biochemical tests also revealed that total protein, albumin, and globulin levels significantly decreased with the interactions of ABS. Red blood cells form vital erythrocyte mass blocks in the presence of ABS [Ulus 2011]. The basic mechanism of action for ABS appears to be the formation of an encapsulated protein network for erythrocyte aggregation.…”