Surgical Anatomy of the Gastrointestinal Tract and Its Vasculature in the Laboratory Rat

Abstract

The aim of this study was to describe and illustrate the morphology of the stomach, liver, intestine, and their vasculature to support the planning of surgical therapeutic methods in abdominal cavity. On adult Wistar rats corrosion casts were prepared from the arterial system and Duracryl Dental and PUR SP were used as a casting medium and was performed macroscopic anatomical dissection of the stomach, liver, and intestine was performed. The rat stomach was a large, semilunar shaped sac with composite lining. On the stomach was very marked fundus, which formed a blind sac (saccus cecus). The rat liver was divided into six lobes, but without gall bladder. Intestine of the rat was simple, but cecum had a shape as a stomach. The following variations were observed in the origin of the cranial mesenteric artery. On the corrosion cast specimens we noticed the presence of the anastomosis between middle colic artery (a. colica media) and left colic artery (a. colica sinistra). We investigated the second anastomosis between middle colic artery and left colic artery. The results of this study reveal that the functional anatomical relationship between the rat stomach, liver and intestine is important for the development of surgical research in human and veterinary medicine.

An objective in vivo diagnostic method for inflammatory bowel disease

“…Prior to surgery, animals were fasted overnight to reduce the amount of gut contents. On the day of surgery, rats were anaesthetized (2% isoflurane, 1% oxygen, flow rate of 1–1.5 l min −1 ; pre-operative analgesia carprofen 50 mg kg −1 subcutaneous), the abdominal cavity was opened and an 8 cm segment of jejunum was selected approximately 30 cm proximal to the ileo-caecal junction [ 22 ]. Four platinum (Pt) ball electrodes (1.2 mm diameter; exposed surface area 3.2 mm²), placed 2 cm apart, were inserted into the lumen and secured with sutures (7-0 silk, Ethicon; figure 2 a ).…”

Effect of different bariatric surgeries on dietary protein bioavailability in rats

“…1). The ileum was defined as the 10 cm before the cecum to recover a sufficient number of samples, although ileum might be shorter, according to Vdoviaková et al (58). Segments were rinsed with NaCl solution (9 g/1,000 ml), and the contents were collected in their entirety, weighed, and stored at Ϫ20°C.…”

Formulation of Tetrandrine Beads Using Ionic Gelation Method Ca-Pectinate Coated Ph-Sensitive Polymers as Colon-Targeted Dosage Form

“…In rat 1, we found beads in the gastric with the coat still could be found; in rat 2, we found beads in the intestine in 64 cm distance from the end of gastric (antrum) with the thin coat still could be found; then in rat 3, we found beads in the intestine in 90 cm distance from the end of gastric (antrum), beads in this condition found expanded and we could not find any coat left. According to these results, beads reached an average distance of 77 cm from the end of gastric (antrum), which was still in jejunum part [16]. Beads found in rat gastrointestinal tract showed coated beads toleration against pH of the upper gastrointestinal tract.…”