Surgical Anatomy of Rats for the Training of Microvascular Anastomosis

Mikami, Takeshi; Suzuki, Hime; Ukai, Ryo; Komatsu, Katsuya; Kimura, Yusuke; Akiyama, Yukinori; Wanibuchi, Masahiko; Mikuni, Nobuhiro

doi:10.1016/j.wneu.2018.09.071

Cited by 14 publications

(6 citation statements)

References 18 publications

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“…La disección aguda de la fascia cervical en la línea media expuso el músculo esternohioideo, que es el punto de referencia en la línea media. 5 Con disección roma se separó la fascia cervical media, que se encuentra entre los músculos infrahioideos y el músculo esternomastoideo. 5 El músculo digástrico se encuentra en el lado craneal del músculo esternohioideo, es necesario utilizar retractores laterales para exponer este último.…”

Section: Técnica Quirúrgicaunclassified

“…5 Con disección roma se separó la fascia cervical media, que se encuentra entre los músculos infrahioideos y el músculo esternomastoideo. 5 El músculo digástrico se encuentra en el lado craneal del músculo esternohioideo, es necesario utilizar retractores laterales para exponer este último. El espacio delimitado por el músculo esternohioideo, esternomastoideo y el músculo digástrico es el triángulo carotídeo (Fig.…”

Section: Técnica Quirúrgicaunclassified

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Remodelado Histológico en Aneurismas Fusiformes en Ratas Wistar tratadas con Wrapping de Algodón y Politetrafluoroetileno

Baldoncini¹

2021

Rev. Argent. Neuroc.

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Intoducción: El tratamiento microquirúrgico para los aneurismas intracraneales es el clipado, sin embargo, algunos aneurismas deben ser resueltos mediante wrapping. El objetivo del trabajo es analizar los cambios histológicos en aneurismas fusiformes en ratas luego del wrapping con algodón natural y politetrafluoroetileno. Materiales y Método:Se trabajó con 12 Ratas Wistar divididas en 3 grupos. Luego de la anestesia se expusieron ambas arterias carótidas comunes, realizándose un aneurisma fusiforme en cada vaso. Se realizó wrapping a las carótidas derechas, grupo 1 con membrana de politetrafluoroetileno, grupo 2 con algodón natural y grupo 3 empleándo combinación de ambos materiales. Los animales fueron sacrificados a los 45 días postoperatorios, para luego procesar las muestras y análisis histológico vascular. Resultados:En el grupo 1 no se evidenciaron modificaciones estructurales, en el grupo 2 se destacó la presencia de células gigantes multinucleadas, inflamación, con infiltrado linfoplasmocitario. En el grupo 3 fue similar al grupo 2 con el agregado de metaplasia condroide y calcificaciones en capa media. Las arterias carotidas izquierdas (control) no presentaron cambios histológicos y a nivel muscular, aumento del tejido conectivo entre las fibras musculares y fibroblastos en el grupo 2. Discusion:Existen publicaciones sobre la técnica de wrapping con materiales autólogos o heterólogos. Sin embargo, no existen análisis experimentales de los efectos microestructurales producidos en las arterias tras la creación y tratamiento de un aneurisma fusiforme. Conclusiones:La combinación más efectiva para reforzar la pared del aneurisma y evitar la reacción inflamatoria circundante es la utilización de algodón natural y politetrafluoroetileno.

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Section: Técnica Quirúrgicaunclassified

Remodelado Histológico en Aneurismas Fusiformes en Ratas Wistar tratadas con Wrapping de Algodón y Politetrafluoroetileno

Baldoncini¹

2021

Rev. Argent. Neuroc.

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“…In our study, maturation of the microvascular anastomosis was monitored in a rat model. An end-to-end anastomosis of the rat common carotid artery (CCA) acts as a suitable laboratory microanastomosis model [3].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Microvascular Anastomosis Maturation in Continuous and Interrupted Suture Technique.

Dostál

Klein

Blassová

et al. 2022

Preprint

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BACKGROUND Use of interrupted sutures in microvascular anastomosis is widely accepted standard technique. Usage of continuous running suture (CRS) is less common due to the presumption that its mechanical firmness can negatively affect anastomosis maturation, but shorter surgical time can be an important factor in the prevention of tissue ischemia. Purpose of this study was to determine whether the use of CRS allows microvascular anastomosis maturation. MATERIAL AND METHODS A rat common carotid artery (CCA) end-to-end microanastomosis model was used, with 19 Long-Evans rats in the interrupted suture group and 13 Long-Evans rats in the CRS group. An immediate blood flow of the operated and the contralateral intact CCA was compared to each other at the time of the anastomosis completion and after 14 days. Quantitative transit time flowmetry measurement and histological examination were used. RESULTS Initial blood flow in both intact CCAs was equal in all animals (p =.004). In the interrupted suture group, the median anastomosis blood flow was 88.9% of the contralateral CCA, median time needed for the suture was 46 minutes. The blood flow after two weeks increased to 96.1%. In the CRS group, the median anastomosis blood flow was 88.3% of the contralateral CCA, median time needed for the suture was 30 minutes. The blood flow after two weeks increased to 100.0%. Achieved suture time reduction was 34.8% (p <.001). Histological examination showed a mature scar. CONCLUSIONS In our study, CRS and interrupted suture microanastomosis maturation rate was comparable. Use of CRS allowed a significant surgical time reduction.

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“…However, the use of biological materials is regulated by international and national standards of experimentation, with handling demanding complex and costly logistics for institutions and students, by requiring, for example, the use of anesthetics, approval by the review board, and guarantee of a place to protect the animals 6 . Furthermore, the ethical establishment of the process of using in vivo models for educational purposes involves following the principle of the 3Rs, “refinement, replacement, and reduction”, and the active search for the reduction in the use of animals 7 , 8 .…”

Section: Introductionmentioning

confidence: 99%

Low cost and easy acquisition: corn grain in microsurgery training

Fernandes

Tramontin

Pimentel

et al. 2022

Rev. Col. Bras. Cir.

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Objectives: develop an easily accessible model for training the initial motor practice in microsurgery using corn kernels. Methods: ten corn kernels (Zea mays) were used. A 7mm longitudinal cut was made on one side of the corn grain. The training consisted of performing 4 simple knots between the edges of the incision, using 10-0 mononylon thread. The parameters analyzed were 1) cost of the model; 2) assembly time of the model test system; 3) time for performing the knots; 4) distance between the knots. Results: in all corn kernels tested, it was possible to perform the proposed microsurgical suture training, without any difficulty in the procedure. The average time to perform the 4 knots was 6.51±1.18 minutes. The total cost of the simulator model was R$3.59. The average distance between the knots was 1.7±0.3mm. The model developed from corn grains has an extremely low cost when compared to the use of animals or high-tech simulators. Other advantages are the easy availability of canned corn kernels and the possibility of making more than four knots along the 7mm incision. Conclusion: the training model developed has low cost, is easy to acquire and viable for training basic manual skills in microsurgery.

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Surgical Anatomy of Rats for the Training of Microvascular Anastomosis

Cited by 14 publications

References 18 publications

Remodelado Histológico en Aneurismas Fusiformes en Ratas Wistar tratadas con Wrapping de Algodón y Politetrafluoroetileno

Remodelado Histológico en Aneurismas Fusiformes en Ratas Wistar tratadas con Wrapping de Algodón y Politetrafluoroetileno

Comparison of the Microvascular Anastomosis Maturation in Continuous and Interrupted Suture Technique.

Low cost and easy acquisition: corn grain in microsurgery training

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