“…The pH of the buffer is critical since the degree of binding is known to vary with pH * Correspondence. (Williamson, 1969), this may also account for some of the variation in previously determined values (Table l), consequently the buffer used was at physiological pH since calcium citrated-plasma was used, Caz+ variations were not considered likely to affect the results. Preliminary studies showed that, with shaking, equilibrium was achieved between 48 and 72 h and that no dilution of the plasma occured and that the pH at equilibrium was 7.4.…”