Surfactin-reinforced gelatin methacrylate hydrogel accelerates diabetic wound healing by regulating the macrophage polarization and promoting angiogenesis

Lü, Yan; Han, Kai; Pang, Bing; Han, Jing; Zhao, Xixi; Xu, Xiaoguang; Jiang, Chunmei; Cui, Ning; Lu, Tingli; Shi, Junling

doi:10.1016/j.cej.2021.128836

Cited by 68 publications

(39 citation statements)

References 62 publications

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“…Gelatin methacryloyl (GelMA) was synthesized as reported in reference [33]. Typically, 1.0 g gelatin was dissolved in 10.0 mL phosphate buffered solution (PBS) at 50 ℃ for 1 h and stirred until forming a clear solution.…”

Section: Synthesis Of Gelatin Methacryloyl (Gelma)mentioning

confidence: 99%

“…Gelatin methacryloyl (GelMA) is a gelatin derivative modi ed by methacryloyl groups [33]. It can form covalently crosslinked hydrogels through a UV photoinitiated radical polymerization with the presence of a photoinitiator while the unique properties of gelatins are retained [34].…”

Section: Introductionmentioning

confidence: 99%

“…It can form covalently crosslinked hydrogels through a UV photoinitiated radical polymerization with the presence of a photoinitiator while the unique properties of gelatins are retained [34]. A large number of arginineglycine-aspartic acid (RGD) sequences in GelMA hydrogels make it excellent in promoting cell attachment and adhesion [33,35]. Moreover, GelMA hydrogels have been reported to have the ability to promote the formation of vascular networks in vitro and in vivo [36].…”

Section: Introductionmentioning

confidence: 99%

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Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

Zhang

Pan

et al. 2021

Preprint

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Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/ hydrogel core-shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15-80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core-shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (D, L-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/ hydrogel core-shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing.

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Section: Synthesis Of Gelatin Methacryloyl (Gelma)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

Zhang

Pan

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Gelatin methacryloyl (GelMA) is a gelatin derivative modified by methacryloyl groups [ 32 , 33 ]. It can form covalently crosslinked hydrogels through a UV photoinitiated radical polymerization with the presence of a photoinitiator while the unique properties of gelatins are retained [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

“…It can form covalently crosslinked hydrogels through a UV photoinitiated radical polymerization with the presence of a photoinitiator while the unique properties of gelatins are retained [ 34 ]. A large number of arginine-glycine-aspartic acid (RGD) sequences in GelMA hydrogels make it excellent in promoting cell attachment and adhesion [ 32 , 35 ]. Moreover, GelMA hydrogels have been reported to have the ability to promote the formation of vascular networks in vitro and in vivo [ 36 ].…”

Section: Introductionmentioning

confidence: 99%

Nanofiber/hydrogel core–shell scaffolds with three-dimensional multilayer patterned structure for accelerating diabetic wound healing

Zhang

Pan

et al. 2022

J Nanobiotechnol

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Impaired angiogenesis is one of the predominant reasons for non-healing diabetic wounds. Herein, a nanofiber/hydrogel core–shell scaffold with three-dimensional (3D) multilayer patterned structure (3D-PT-P/GM) was introduced for promoting diabetic wound healing with improved angiogenesis. The results showed that the 3D-PT-P/GM scaffolds possessed multilayered structure with interlayer spacing of about 15–80 μm, and the hexagonal micropatterned structures were uniformly distributed on the surface of each layer. The nanofibers in the scaffold exhibited distinct core–shell structures with Gelatin methacryloyl (GelMA) hydrogel as the shell and Poly (d, l-lactic acid) (PDLLA) as the core. The results showed that the porosity, water retention time and water vapor permeability of the 3D-PT-P/GM scaffolds increased to 1.6 times, 21 times, and 1.9 times than that of the two-dimensional (2D) PDLLA nanofibrous scaffolds, respectively. The in vitro studies showed that the 3D-PT-P/GM scaffolds could significantly promote cell adhesion, proliferation, infiltration and migration throughout the scaffolds, and the expression of cellular communication protein-related genes, as well as angiogenesis-related genes in the same group, was remarkably upregulated. The in vivo results further demonstrated that the 3D-PT-P/GM scaffolds could not only effectively absorb exudate and provide a moist environment for the wound sites, but also significantly promote the formation of a 3D network of capillaries. As a result, the healing of diabetic wounds was accelerated with enhanced angiogenesis, granulation tissue formation, and collagen deposition. These results indicate that nanofiber/hydrogel core–shell scaffolds with 3D multilayer patterned structures could provide a new strategy for facilitating chronic wound healing. Graphical Abstract

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Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

Mao

Chen

Cai

et al. 2021

Adv Funct Materials

107

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Macrophage immunotherapy is an emerging treatment strategy that modulates the immune system to promote wound healing. The functionality and regeneration of tissue depend on spatially and temporally regulating the biophysical and biochemical microenvironmental with poorly understood mechanisms. Biomaterials are carefully crafted to display and deliver macrophage regulatory signals in a precise and near‐physiological way, serving as powerful artificial microenvironments in which to explore and direct the fate of macrophages. The review starts with discussing the classification and function of macrophages, and then introduces the polarization of macrophages in different microenvironments of conventional wounds and chronic wounds. Recent advances in biomaterials that balance the phenotypes of macrophages in wound healing are emphasized. Finally, looking ahead, the potential ability of biomaterial scaffolds to modulate immune signaling to produce an environment conducive to regeneration is discussed.

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Surfactin-reinforced gelatin methacrylate hydrogel accelerates diabetic wound healing by regulating the macrophage polarization and promoting angiogenesis

Cited by 68 publications

References 62 publications

Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

Nanofiber/hydrogel Core-shell Scaffolds With Three-dimensional Multilayer Patterned Structure for Accelerating Diabetic Wound Healing

Nanofiber/hydrogel core–shell scaffolds with three-dimensional multilayer patterned structure for accelerating diabetic wound healing

Advanced Biomaterials for Regulating Polarization of Macrophages in Wound Healing

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