Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms

Wang, Youyou; Zhang, Congying; Ji, Liang; Wang, Le; Gao, Wenbin; Jiang, Jizhi; Chang, Ruixue

doi:10.1007/s00253-020-10773-y

Cited by 42 publications

(24 citation statements)

References 52 publications

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“…The major Bacillus spp. that has been applied in biological control are B. subtilis , B. megaterium , B. amyloliquefaciens , and B. velezensis ( Wang Y. et al, 2020 ; Zeng et al, 2021 ). Bacillus is a well-known producer of a wide array of antagonistic compounds with different structures, namely, peptides, lipopeptides, bacteriocins, and polyketide compounds ( Fira et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community

Jiang²,

An³

et al. 2022

Front. Microbiol.

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The occurrence of plant diseases is closely associated with the imbalance of plant tissue microecological environment. The regulation of the phyllosphere microbial communities has become a new and alternative approach to the biological control of foliar diseases. In this study, Bacillus velezensis SYL-3 isolated from Luzhou exhibited an effective inhibitory effect against Alternaria alternata and tobacco mosaic virus (TMV). The analysis of phyllosphere microbiome by PacBio sequencing indicated that SYL-3 treatment significantly altered fungal and bacterial communities on the leaves of Nicotiana tabacum plants and reduced the disease index caused by A. alternata and TMV. Specifically, the abundance of P. seudomo, Sphingomonas, Massilia, and Cladosporium in the SYL-3 treatment group increased by 19.00, 9.49, 3.34, and 12.29%, respectively, while the abundances of Pantoea, Enterobacter, Sampaiozyma, and Rachicladosporium were reduced. Moreover, the abundance of beneficial bacteria, such as Pseudomonas and Sphingomonas, was negatively correlated with the disease indexes of A. alternata and TMV. The PICRUSt data also predicted the composition of functional genes, with significant differences being apparent between SYL-3 and the control treatment group. Further functional analysis of the microbiome also showed that SYL-3 may induce host disease resistance by motivating host defense-related pathways. These results collectively indicate that SYL-3 may suppress disease progression caused by A. alternata or TMV by improving the microbial community composition on tobacco leaves.

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Section: Introductionmentioning

confidence: 99%

Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community

Jiang²,

An³

et al. 2022

Front. Microbiol.

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“…The statistical analysis was performed using GraphPad Prism 7 software by one-way ANOVA with the Dunnett test (P < 0.05). The variation was recorded as mean ± SE (****: P < 0.0001, ***: P < 0.001, **: P < 0.01, *: P < 0.05) (Ongena et al 2007;Wang et al 2020). The genes related to plant growth promotion, such as ysnE, ywkB, phyC, dhb operon, and bltD, synthesize IAA, auxin, phytase, siderophore, and spermidine, respectively (Kerovuo et al 1998;May et al 2001;Baichoo et al 2002;Quentin et al 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Bacillus subtilis has been widely used in agricultural production due to its environmental safety, straightforward industrial production, and good biocontrol efficacy (Wang et al 2020). The development of genome sequencing technology and bioinformatic analysis have made it convenient to obtain genomic information on B. subtilis, allowing us to comprehensively understand its life activities (Moszer 1998).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive genomic analysis of Bacillus subtilis 9407 reveals its biocontrol potential against bacterial fruit blotch

Zeng

Wang

et al. 2021

Phytopathol Res

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Bacillus subtilis, a plant-beneficial bacterial species exhibiting good biocontrol capabilities, has been widely used in agricultural production. The endophytic strain 9407 can efficiently control bacterial fruit blotch (BFB) caused by the gram-negative bacterium Acidovorax citrulli. However, the mechanism underlying its biocontrol ability remains poorly understood. Given the genomic diversity of B. subtilis, strain 9407 was sequenced and assembled in this study to determine the genome information associated with its biocontrol traits. A combination of core genome phylogenetic analysis and average nucleotide identity (ANI) analysis demonstrated that the 9407 strain belonged to B. subtilis. Various functional genes related to biocontrol traits, i.e., biofilm formation, motility, pathogen inhibition, plant growth promotion, and induction of systemic resistance, were identified in B. subtilis 9407. Four secondary metabolite biosynthesis gene clusters with antibacterial ability were also found in the B. subtilis 9407 genome, including newly identified subtilosin A, bacilysin, and bacillaene, and the previously reported surfactin. Mutants lacking sboA or bacG, which are defective in synthesizing subtilosin A or bacilysin, showed decreased inhibitory activity against A. citrulli MH21, and the triple mutant with deleted sboA, bacG, and srfAB almost completely lost its inhibitory activity. The biofilm formation and swarming motility of the sboA and bacG mutants also decreased, in turn leading to decreased colonization on melon roots and leaves. Under greenhouse conditions, the biocontrol efficacy of the sboA and bacG mutants against BFB on melon leaves decreased by 21.4 and 32.3%, respectively. Here, we report a new biocontrol pathway of B. subtilis 9407 against BFB, in which subtilosin A and bacilysin contributed to the biocontrol efficacy by improving antibacterial activity and colonization ability of the strain. The comprehensive genomic analysis of B. subtilis 9407 improves our understanding of the biocontrol mechanisms of B. subtilis, providing support for further research of its biocontrol mechanisms and field applications.

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“…For detached leaf experiments, tomato plants were cultivated for 50 days, and the leaves (the 6th to the 9th leaf) were harvested and then placed in Petri dishes. 50 After preparing aqueous solutions of BH and the fungicide nanoplatform at different concentrations (60 and 120 μg mL −1 , respectively), the obtained fungicide solution of each treatment (50 μL) was smeared on the surface of each leaf, respectively. For comparison, the tomato leaf treated with deionized water (50 μL) was used as a control.…”

Section: Methodsmentioning

confidence: 99%

Supramolecular Nanoplatform Based on Mesoporous Silica Nanocarriers and Pillararene Nanogates for Fungus Control

Wang

Lou

Cai

et al. 2021

ACS Appl. Mater. Interfaces

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Synthetic fungicides have been widely used to protect crops from fungal diseases. However, excessive use of synthetic fungicides leads to the generation of fungicide resistance in fungal pathogens. Recently, smart cargo delivery systems have been introduced for the construction of a pesticide delivery nanoplatform, benefiting from their controlled release performance. Herein, a fungal pathogen microenvironment-responsive supramolecular fungicide nanoplatform has been designed and constructed, using quaternary ammonium salt (Q)-modified mesoporous silica nanoparticles (MSN-Q NPs) as nanocarriers loaded with berberine hydrochloride (BH) and carboxylatopillar[5]arene (CP[5]A) as nanogates to form BH-loaded CP[5]A@MSN-Q NPs for effective inhibition of Botrytis cinerea. CP[5]A as nanogates can endow the fungicide nanoplatform with pH stimuli-responsive release features for the control of fungicide release. The loaded BH, as a natural plant fungicide, provides an ecofriendly alternative to synthetic fungicides for controlling B. cinerea. Interestingly, we use oxalic acid (OA) secreted by B. cinerea as a trigger so that BH can be released from the fungicide nanoplatform on demand under pathogen microenvironments for controlling B. cinerea. The experimental results indicate that the fabricated fungicide nanoplatform could effectively inhibit the mycelial growth and spore germination, providing a new way for the management of B. cinerea in actual application.

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Surfactin and fengycin B extracted from Bacillus pumilus W-7 provide protection against potato late blight via distinct and synergistic mechanisms

Cited by 42 publications

References 52 publications

Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community

Bacillus velezensis SYL-3 suppresses Alternaria alternata and tobacco mosaic virus infecting Nicotiana tabacum by regulating the phyllosphere microbial community

Comprehensive genomic analysis of Bacillus subtilis 9407 reveals its biocontrol potential against bacterial fruit blotch

Supramolecular Nanoplatform Based on Mesoporous Silica Nanocarriers and Pillararene Nanogates for Fungus Control

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