Surfactant stimulation of growth in cultured plant cells, tissues and organs

Lowe, K. C.; Davey, M. R.; Laouar, Leila; Khatun, Asma; Ribeiro, R.C.S.; Power, J. B.; Mulligan, B. J.

doi:10.1007/978-94-011-0790-7_23

Cited by 14 publications

(5 citation statements)

References 23 publications

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“…These findings revealed that plant morphogenesis is genotype dependent, which is not only influenced by exogenous PGR action but is also interlinked with PGR synthesis and nutrient uptake (Rubio et al, 2009). Inclusion of regeneration enhancers in culture medium has been reported to be useful for shoot formation and proliferation (Lowe et al, 1994). In the current study, the inclusion of a lower concentration of Pluronic F-68 substantially increased the shoot number and frequency.…”

Section: Discussionmentioning

confidence: 98%

“…However, recently, TDZ (a phenylurea-type cytokinin) has been commonly used to promote shoot proliferation and regeneration in various plant species (Faisal et al, 2014) and can replace the use of BAP. In addition, supplementation of regeneration enhancers such as Pluronic F-68 and AgNO 3 in PGRs added culture medium plays an important role to promote multiple shoot production (Lowe et al, 1994;Yildirim and Turker, 2014). However, to our knowledge, there are no published reports on multiple shoot production from cotyledonary nodes of okra using different concentrations and combinations of PGRs and regeneration enhancers.…”

mentioning

confidence: 99%

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Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

Irshad¹,

Rizwan²,

Debnath³

et al. 2018

horts

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The regeneration frequency of okra (Abelmoschus esculentus) is greatly influenced by its genetic makeup and recalcitrant nature. Phenolic secretion, in particular, is a major problem in okra tissue culture. This study describes a reproducible, rapid, and more efficient in vitro regeneration method using cotyledonary node explants of okra. Explants were incubated on Murashige and Skoog (MS) medium containing different concentrations and combinations of various plant growth regulators (PGRs) [benzyladenine (BA), thidiazuron (TDZ), and α-naphthylacetic acid (NAA)], and regeneration enhancers [silver nitrate (AgNO3) and Pluronic F-68]. Cut ends of cotyledonary node segments rapidly turned brown and cultures failed to establish. Antibrowning additives, such as activated charcoal (AC), ascorbic acid (AA), and AgNO3 at various concentrations in PGR-free MS basal medium were tested for their ability to control phenolic secretion from explants. Among these additives, 15 mg·L−1 AA was found to be optimal for controlling phenolic secretion, resulting in healthy explants and culture establishment. The highest number of shoots (a mean of 9.3 ± 0.9 shoots per cotyledonary node explant) was obtained on MS media containing 0.5 mg·L−1 NAA + 1 mg·L−1 TDZ + 0.1% Pluronic F-68. Individual shoots were elongated on MS medium + 1 mg·L−1 BA + 0.1 mg·L−1 gibberellic acid (GA3) (shoot length 5.3 ± 0.2 cm) and rooted on ½ MS medium + 1 mg·L−1 indole-3-butyric acid (IBA) and 200 mg·L−1 AC (5.3 ± 0.2 roots per shoot). Rooted plantlets were acclimatized in plastic pots inside a plant growth chamber at 25 ± 2 °C and 70% relative humidity, with an 80% survival rate. This optimized protocol can be used for producing transgenic plants of commercial okra cultivars through genetic transformation.

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Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

Irshad¹,

Rizwan²,

Debnath³

et al. 2018

horts

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“…It is possible, however, that a low-dose application of nonionic surfactant applied directly to seed will improve uptake of other seed treatments. Research efforts to regenerate whole plants has suggested that nonionic surfactants may influence shoot regeneration frequency by increasing the permeability of the plasma membrane to growth regulators and/or nutrients by creating transient increases in membrane fluidity (Grant and Hammatt, 2000;Lowe et al, 1994). It may be plausible that seed absorption of various biostimulants such as plant growth regulators, fungicides, and fertilizers could be improved by adding a nonionic surfactant within the seed treatment formulation.…”

Section: Discussionmentioning

confidence: 99%

Low-dose Application of Nonionic Alkyl Terminated Block Copolymer Surfactant Enhances Turfgrass Seed Germination and Plant Growth

Madsen

Fidanza

Barney

et al. 2016

hortte

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Rapid seed germination and vigorous seedling growth are desired when establishing turfgrass lawns from seed. Low-dose concentrations of nonionic, block copolymer surfactants can have a direct effect on plant physiological functions and growth. The objectives were to determine if a low-dose application of a nonionic alkyl ended block copolymer surfactant applied directly to the seed, within a film coating, would 1) influence speed, synchrony, and final germination percentage (FGP), and 2) enhance seedling emergence and the speed of turfgrass establishment under deficit irrigation. Tests were performed with tall fescue (Schedonorus arundinaceus) and perennial ryegrass (Lolium perenne). Surfactant was applied directly to the seed using a rotary seedcoater at 0.1% by weight of seed. In the first experiment, germination was compared between seeds with a surfactant film coating (SFC) and untreated seeds in growth chambers at three different constant temperatures (10, 20, and 30 °C). For both species, the SFC decreased the time for seed germination, and improved germination synchrony, with the greatest treatment response at 10 and 30 °C compared with untreated seed. Application of a SFC did not influence FGP. In the second experiment, untreated and treated seed were compared in a grow-room study, with pots watered weekly to 70% of field capacity (FC). Perennial ryegrass density, cover, and aboveground biomass from the SFC were ≈47%, 48%, and 46% greater than untreated seed, respectively. Tall fescue density, cover, and aboveground biomass from the SFC seeds were ≈22%, 23%, and 28% greater than untreated seed, respectively. Overall these studies demonstrate that SFC can promote seed germination and also enhance turfgrass establishment under deficit irrigation.

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“…Pluronic F 68 (Poloxamer 188/ PF 68) is a non-ionic block copolymer of polyethylene glycol and polypropylene glycol (Elibol, 1999), which has been used to emulsify PFCs for use in oxygen-transport fluids (Johnson et al, 1990). This surfactant has been used as a growth-promoting additive to animal and microbial cultures (Lowe et al, 1994). When a PF 68 concentration of 10mg.ml -1 was used in Perfluorooctyl bromide (PFOB) emulsions, the emulsion was extremely stable and remained in this state for more than 7 days.…”

Section: Pluronic F 68 As a Surfactant And A Biomass Protectormentioning

confidence: 99%

“…When coalescence and ripening are suppressed, the emulsion might remain stable for years (Mason, 1999). PF 68 has also been used as a cytoprotectant and growth promoting additive to animal cell and microbial cultures (Lowe et al, 1994). Microbial cell damage arising from gas sparging is considered to be a major obstacle in the operational longevity of large-scale bioprocesses (Wu, 1995), thus requiring the application of protective additives in the culture medium.…”

Section: Surfactantmentioning

confidence: 99%

Water-immiscible dissolved oxygen carriers in combination with Pluronic F 68 in bioreactors

Ntwampe

Williams²,

Sheldon³

2010

Afr. J. Biotechnol.

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The supply and availability of dissolved oxygen (DO) in aerobic bioprocesses is often a limiting factor for the scaling up, improvement and general performance of these bioprocesses. The use of different DO carriers, particularly the use of perfluorocarbons as oxygen carriers, is discussed in this review. It also highlights interactions of microbial cultures with the surfactant, Pluronic F 68. Although oxygen carriers have been used extensively in the medical field, this review only focuses on their use in microbial bioprocess used for the production of high-value bioproducts. The use of water-immiscible compounds in combination with Pluronic F 68 in bioprocesses is discussed with the intention of analysing their combined effect where bioreactor and biomass performance is affected by DO limitations, nutrient starvation, high concentrations of trace element ions, oxidative stress and cell death from mechanical stress.

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Surfactant stimulation of growth in cultured plant cells, tissues and organs

Cited by 14 publications

References 23 publications

Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

Ascorbic Acid Controls Lethal Browning and Pluronic F-68 Promotes High-frequency Multiple Shoot Regeneration from Cotyldonary Node Explant of Okra (Abelmoschus esculentus L.)

Low-dose Application of Nonionic Alkyl Terminated Block Copolymer Surfactant Enhances Turfgrass Seed Germination and Plant Growth

Water-immiscible dissolved oxygen carriers in combination with Pluronic F 68 in bioreactors

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