Surfactant protein D and asthma

Haczku, Angela Franciska; Vass, G.; Kierstein, S.

doi:10.1111/j.1365-2222.2004.02134.x

Cited by 17 publications

(15 citation statements)

References 37 publications

(49 reference statements)

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“…9–11) [13], SP-D (spot Nos. 16–19) [14], polymeric immunoglobulin receptor (pIgR; spot No. 14) [15] and PSTPIP (proline-serine-threonine phosphatase-interacting protein; spot No.…”

Section: Resultsmentioning

confidence: 99%

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Dexamethasone Alters Bronchoalveolar Lavage Fluid Proteome in a Mouse Asthma Model

Zhao

Yeong

Wong

2006

Int Arch Allergy Immunol

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Background: Glucocorticoid is the most effective anti-inflammatory agent for asthma. The spectrum of protein targets that can be regulated by glucocorticoid in asthma is not fully understood. The present study tried to identify novel protein targets of dexamethasone in allergic airway inflammation by analyzing the proteome of mouse bronchoalveolar lavage (BAL) fluid. Methods: BALB/c mice sensitized and challenged with ovalbumin (OVA) showed increased pulmonary inflammatory cell infiltration, airway mucus production and serum OVA-specific IgE level. Dexamethasone inhibited all these allergic airway inflammation endpoints. BAL fluid proteins were resolved by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: The levels of 26 BAL fluid proteins were found to be markedly altered by dexamethasone. A family of chitinases (Ym1, Ym2 and acidic mammalian chitinase, AMCase), lungkine, gob-5, surfactant protein D and polymeric immunoglobulin receptor have been found for the first time to be downregulated by dexamethasone in allergic airways. The downregulatory effects were confirmed by immunoblotting and RT-PCR analyses. Dexamethasone was also shown to significantly inhibit lavage fluid chitinase bioactivity. In addition, dexamethasone promoted airway expression of vitamin D-binding protein, heptoglobin and α₁-antitrypsin. Conclusions: Among all these newly identified protein targets of dexamethasone, AMCase and gob-5 have been shown to be pro-inflammatory in asthma. Downregulation of AMCase and gob-5 may be considered as two novel anti-inflammatory actions of glucocorticoid in asthma.

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“…9–11) [13], SP-D (spot Nos. 16–19) [14], polymeric immunoglobulin receptor (pIgR; spot No. 14) [15] and PSTPIP (proline-serine-threonine phosphatase-interacting protein; spot No.…”

Section: Resultsmentioning

confidence: 99%

“…SP-D is a member of the collagenous calcium-dependent lectin (collectin) family, and is synthesized and secreted by airway epithelial cells [14]. In a mouse model of asthma, SP-D has been shown to lower blood eosinophilia, pulmonary inflammatory cell infiltration, allergen-specific IgE level and T H 2 cytokine levels [30].…”

Section: Discussionmentioning

confidence: 99%

Dexamethasone Alters Bronchoalveolar Lavage Fluid Proteome in a Mouse Asthma Model

Zhao

Yeong

Wong

2006

Int Arch Allergy Immunol

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“…Sa structure est proche de celle de SP-A car elle appartient aussi à la famille des collectines [29]. Elle est synthétisée par les pneumocytes de type 2 et les cellules épithéliales non ciliées.…”

Section: Les Protéines a Et D Du Surfactant (Sp-a Et Sp-d) Sont Des Punclassified

“…SP-D permet de moduler l'absorption des particules allergéniques [31] car elle lie et séquestre les allergènes inhalés, évitant ainsi la liaison aux IgE. Elle facilite aussi la clairance des allergènes par le biais des macrophages alvéolaires [29]. SP-D permet par ailleurs de réduire l'éosinophilie pulmonaire et sanguine par le biais d'un rétrocontrôle négatif, induisant une régulation du chimiotactisme et de l'infiltration éosinophilique dans le tissu pulmonaire [30].…”

Section: Les Protéines a Et D Du Surfactant (Sp-a Et Sp-d) Sont Des Punclassified

“…SP-D permet par ailleurs de réduire l'éosinophilie pulmonaire et sanguine par le biais d'un rétrocontrôle négatif, induisant une régulation du chimiotactisme et de l'infiltration éosinophilique dans le tissu pulmonaire [30]. L'effet anti-inflammatoire de SP-D correspondrait à un déplacement du profil cytokinique d'une réponse Th2 vers une réponse Th1 protectrice [29,30].…”

Section: Les Protéines a Et D Du Surfactant (Sp-a Et Sp-d) Sont Des Punclassified

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Les pneumoprotéines sériques, de nouveaux biomarqueurs pour phénotyper l’asthme du nourrisson ?

Fontaine¹,

Amat²,

Paluel-Marmont³

et al. 2015

Revue Française d'Allergologie

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Il reste difficile actuellement de distinguer a priori les différents phénotypes d'asthme du nourrisson, et notamment de distinguer l'asthme à risque de persistance et de sévérité de l'asthme transitoire. Il est essentiel d'améliorer la prédiction du pronostic de l'asthme au cours de l'enfance, car l'asthme à début précoce concerne une proportion non négligeable, environ 10 %, de la population générale pédiatrique. L'épithélium pulmonaire sécrète différentes protéines, dont certaines, appelées pneumoprotéines, sont dorénavant dosables dans le sérum. Certaines d'entre elles pourraient constituer une piste intéressante dans la recherche de biomarqueurs associés aux phénotypes de l'asthme. AbstractAt present, it is still difficult to distinguish a priori different phenotypes of early-onset asthma in infants, especially to be able to differentiate infants with asthma who are at risk of persistence and severity from those with transient wheezing. It is thus essential to improve the prediction of the prognosis of early-onset asthma because of its high frequency, about 10%, in the pediatric general population. The pulmonary epithelium secretes into the serum a variety of proteins, including some known as pneumoproteins, and these proteins can now be assayed in the serum. Some of them might represent an interesting way in the search of new biomarkers associated with particular asthma phenotypes.

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