SURFACTANT PROTEIN-B (SP-B) PROMOTER IN REPLICATION DEFICIENT ADENOVIRUS(rAd) DIRECTS PULMONARY EPITHELIAL CELL-SPECIFIC GENE EXPRESSION. • 1986

Guttentag, Susan H.; Strayer, Marlene S.; Ballard, Philip L.

doi:10.1203/00006450-199604001-02010

Pediatr Res

1996

DOI: 10.1203/00006450-199604001-02010

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SURFACTANT PROTEIN-B (SP-B) PROMOTER IN REPLICATION DEFICIENT ADENOVIRUS(rAd) DIRECTS PULMONARY EPITHELIAL CELL-SPECIFIC GENE EXPRESSION. • 1986

Susan H. Guttentag

Marlene S. Strayer

Philip L. Ballard

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1998

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“…Our results indicate that the SP-B promoter drives lung tissue-and cell-type-specific transgene expression in vitro and in vivo . A preliminary report has been published (26).…”

mentioning

confidence: 99%

Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter

Strayer

Guttentag

Ballard

1998

Am J Respir Cell Mol Biol

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We examined the ability of the human surfactant protein B (SP-B) promoter to confer cell specificity of transgene expression in an adenoviral vector. Using similar replication-deficient adenoviruses (rAd), we compared lacZ reporter gene expression driven by the human SP-B promoter (rAd.SPBlacZ) with the ubiquitously expressed Rous sarcoma virus promoter (rAd.RSVlacZ). rAd.SPBlacZ expressed lacZ in H-441 and A549 lung epithelial cell lines and not in HeLa cells whereas rAd.RSVlacZ expressed in all three cell lines. In primary human fetal lung fibroblasts, beta-galactosidase activity from rAd.RSVlacZ transduction increased in a dose-dependent manner whereas activity from rAd.SPBlacZ remained low. In mixed cell cultures prepared from human fetal lung explants that contained fibroblasts and type II cells, X-Gal staining localized rAd.SPBlacZ expression to only type II cells whereas rAd.RSVlacZ expressed in both cell types. In 24-wk gestation human fetal tissue explants infected ex vivo, the RSV promoter directed lacZ expression in lung, trachea, heart, liver, and esophagus, whereas with the SP-B promoter lacZ was expressed only in lung, specifically in air space-lining cells. This specificity was maintained in vivo. lacZ expression was undetectable in lung and other tissues after intravenous administration of rAd.SPBlacZ whereas rAd.RSV-lacZ expressed primarily in liver. After intratracheal instillation of rAd.SPBlacZ into mice, X-Gal staining localized expression to type II and Clara cells. In contrast, rAd.RSVlacZ expressed in all pulmonary epithelial cell types. Our results indicate that the SP-B promoter may be useful in targeting type II and Clara cells for gene therapy of conditions such as inherited deficiency of SP-B.

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“…Our results indicate that the SP-B promoter drives lung tissue-and cell-type-specific transgene expression in vitro and in vivo . A preliminary report has been published (26).…”

mentioning

confidence: 99%

Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter

Strayer

Guttentag

Ballard

1998

Am J Respir Cell Mol Biol

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SURFACTANT PROTEIN-B (SP-B) PROMOTER IN REPLICATION DEFICIENT ADENOVIRUS(rAd) DIRECTS PULMONARY EPITHELIAL CELL-SPECIFIC GENE EXPRESSION. • 1986

Cited by 1 publication

References 0 publications

Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter

Targeting Type II and Clara Cells for Adenovirus-mediated Gene Transfer Using the Surfactant Protein B Promoter

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