Surfactant levels after reversible tracheal occlusion and prenatal steroids in experimental diaphragmatic hernia

Bratu, Ioana; Flageole, Hélène; Laberge, Jean-Martin; Possmayer, Fred; Harbottle, Richard P.; Kay, Saundra; Khalifé, Samir; Piedboeuf, Bruno

doi:10.1053/jpsu.2001.20027

Cited by 49 publications

(41 citation statements)

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“…temporary TO) decreases the levels of lung tissue stretch, leading to increased expression of surfactant proteins and density of AE2 cells (3). However, respiratory gas exchange and CL remained abnormal in lambs with DH that underwent temporary TO, possibly due to impaired synthesis and/or secretion of surfactant (11).…”

Section: Discussionmentioning

confidence: 95%

“…Prolonged fetal TO (i.e. Ͼ10 d) has unfavorable effects upon lung development including an increase in alveolar wall thickness (3,10), decreased AE2 cell density (3,7,11) and reduced synthesis of pulmonary surfactant (12). Functionally, lambs that have undergone prolonged periods of TO (4 wk) to reverse fetal lung hypoplasia demonstrate low CL and are difficult to ventilate despite restoration of lung growth (3,12,13).…”

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confidence: 99%

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Prenatal Glucocorticoids and Exogenous Surfactant Therapy Improve Respiratory Function in Lambs with Severe Diaphragmatic Hernia Following Fetal Tracheal Occlusion

et al. 2006

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Fetal tracheal occlusion (TO) accelerates lung growth and can reverse severe lung hypoplasia associated with diaphragmatic hernia (DH), however, lung compliance (CL) and respiratory gas exchange remain abnormal. We determined the individual and combined effects of prenatal glucocorticoids (GC) and exogenous surfactant therapy (S) on postnatal pulmonary function in lambs with DH that underwent prolonged TO. DH was created in 22 fetal sheep at 65 d of gestation and TO performed at 110 d. Eleven DH/TO animals received prenatal GC (betamethasone, 0.5 mg/kg) 48 h before delivery; six GC-treated and five non-GC lambs were administered surfactant (Infasurf, 3 mg/kg) at birth. Six sham-operated lambs served as controls. Lambs were delivered at 139 d gestation and ventilated for 2 h. GC or surfactant therapy alone significantly improved respiratory gas exchange, CL, and ventilatory efficiency index. Total lung capacity was normalized only in DH/TO lambs that received both GC and S. (Pediatr Res 60: 131-135, 2006)

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Section: Discussionmentioning

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Prenatal Glucocorticoids and Exogenous Surfactant Therapy Improve Respiratory Function in Lambs with Severe Diaphragmatic Hernia Following Fetal Tracheal Occlusion

et al. 2006

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“…It is now evident that restoring the normal egress of liquid from the lung after a period of in utero TO (i.e., temporary TO) leads to the recovery of AE2 cells and their ability to produce surfactant (5,7,11,14,17,21). Because administration of exogenous surfactant markedly improves postnatal lung function after prolonged TO (25), we hypothesized that temporary TO in fetuses with severe lung hypoplasia would 1) increase the surface area available for respiratory gas exchange at birth, 2) lead to recovery of AE2 cell density and surfactant protein (SP) mRNA expression, and 3) improve postnatal lung function above that of animals that underwent prolonged TO.…”

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Temporary tracheal occlusion in fetal sheep with lung hypoplasia does not improve postnatal lung function

Davey

Hedrick

Bouchard

et al. 2003

Journal of Applied Physiology

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“…Akciğer hipoplazisini geri çevirmeyi hedefleyen trakeal oklüzyon fikri buradan ortaya çıkmıştır. Literatürde hayvan modellerinde intrauterin dönemde gerçekleştirilen trakeal oklüzyonun akciğer hipoplazisini morfolojik olarak geri çevirdiğini ve postnatal dönemde akciğer fonksiyonlarını ve akciğer kompliansını arttırdığını gösteren çalışmalar mevcuttur (5)(6)(7)(8)(9)(10). Çalışmamızda trakeal oklüzyona ek olarak büyüme faktörleri, steroid ve surfaktan gibi akciğer gelişiminde rol oynayan etkenleri bir araya getirmeyi amaçladık.…”

Section: Introductionunclassified

To Analyse Effect of Tracheal Occlusion in the Development of Lung in Nitrofen Induced Congential Diaphragmatic Hernia Model

Aydın¹,

Yener²,

Üstündağ³

2016

Haseki

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ÖzAmaç: Doğumsal diyafragma hernisi yüksek morbidite ve mortaliteye sahip doğumsal anomalidir. Çalışmamızda nitrofen modeli ile oluşturulmuş doğumsal diyafragma hernili olgularda trakeal oklüzyonun akciğerlerin gelişimindeki etkisini incelemek amaçlanmaktadır. Yöntemler: Aynı yaş grubunda Wistar albino türü sıçanlar çiftleştirildi. Vajinal smearda sperm gösterilen sıçanlar çalışmaya dahil edildi. Smearda spermin gösterildiği gün sıfırıncı gebelik günü kabul edildi. Gebeliğin dokuzuncu gününde anne sıçana 100 mg nitrofen gavaj verildi ve 18. günde laparotomi ve histerotomi yapıldı. Her iki fetüsten birine servikal yaklaşımla trakeal oklüzyon uygulanırken diğerine trakeal oklüzyon yapılmadı. Gebe sıçanlara 22. gebelik gününde yeniden laparotomi ve histerotomi yapılarak fetüslerin akciğerleri çıkarıldı. Akciğerlerinin gelişimi histopatolojik olarak değerlendirildi. Bulgular: Nitrofen modeli ile 17 fetüsün 10'unda (%59) doğumsal diyafragma hernisi gelişti. Deneklerin beşi kontrol grubunda, beşi trakeal oklüzyon grubunda idi. Beş denekte sağ diyafragma hernisi, üç denekte sol diyafragma hernisi gerçekleşirken iki denekte herni bilateraldi. Akciğer gelişimi kontrol grubunda iki denekte psödoglandüler evrede, iki denekte psödoglandüler evre ve erken kanaliküler evrede, bir denekte ise erken kanaliküler evrede duraklamışken; trakeal oklüzyon grubunda iki denekte geç kanaliküler-erken sakküler evrede, bir denekte sakküler ve iki denekte erken kanaliküler evrede akciğer gelişiminin durakladığı görüldü. Sonuç: Nitrofen modeli doğumsal diyafragma hernisi modeli oluşturmada etkin bir yöntemdir ancak Bochdalek hernisini simüle etmede yetersiz kalmaktadır. Trakeal oklüzyon deneklerin akciğerlerinin gelişimlerini arttırmaktadır. Anahtar Sözcükler: Doğumsal diyafragmatik herni, 2,4 diklorofenil-p -nitrofenil eter, trakeal oklüzyon Aim:Congenital daphragmatic hernia is a congenital abnormality with high morbidity and mortality. In our study, we aimed to analyse the effect of tracheal occlusion in lung development in nitrofen-induced congenital daphragmatic hernia. Method:Wistar albino rats of the same age were mated. The rats, in whom the presence of sperm in vaginal smear was observed, were included in the study. It was zero day of pregnancy. At the ninth day of pregnancy, 100 mg nitrofen was introduced to pregnant rats by gavage and at the 18 th day of pregnancy, laparotomy and hysterotomy were performed. Tracheal occlusion was performed in one of two fetuses. At the 22 nd day of pregnancy, the lungs of the fetuses were excised. Lung development was analysed histopathologically.Results:Ten (59%) of 17 fetuses had congenital daphragmatic hernia. Five of them were in control group. The defect was right-sided in five of the subjects, left-sided in three, and bilateral in two. Lung development was observed to be stopped in pseudoglandular subjects in two of the fetuses, in pseudoglandular-early canalicular stage in two, and early canalicular in one of the fetuses in control group while late canalicularearly saccular in two, saccula...

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Surfactant levels after reversible tracheal occlusion and prenatal steroids in experimental diaphragmatic hernia

Cited by 49 publications

References 36 publications

Prenatal Glucocorticoids and Exogenous Surfactant Therapy Improve Respiratory Function in Lambs with Severe Diaphragmatic Hernia Following Fetal Tracheal Occlusion

Prenatal Glucocorticoids and Exogenous Surfactant Therapy Improve Respiratory Function in Lambs with Severe Diaphragmatic Hernia Following Fetal Tracheal Occlusion

Temporary tracheal occlusion in fetal sheep with lung hypoplasia does not improve postnatal lung function

To Analyse Effect of Tracheal Occlusion in the Development of Lung in Nitrofen Induced Congential Diaphragmatic Hernia Model

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