Surfaces that Adhesively Discriminate Breast Epithelial Cell Lines and Lymphocytes in Buffer and Human Breast Milk

Kalasin, Surachate; Browne, Eva P.; Arcaro, Kathleen F.; Santore, Maria M.

doi:10.1021/acsami.9b03385

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…A 100 ppm solution of poly- l -lysine hydrobromide (PLL, from Sigma-Aldrich, 15 000–30 000 g/mol nominal molecular weight) was flowed through the chamber at a wall shear rate of 5 s –1 for 10 min, more than sufficient to saturate the surface, and then flowing buffer was returned to the chamber. We previously demonstrated that this procedure produces an adsorbed layer of PLL of about 0.4 mg/m 2 , which is flat (within a few nanometers of the surface), and is not removed, in the time frame of a few hours, upon exposure to a buffer of a variety of ionic strengths; , flowing micro- or nanoparticles; and a variety of negatively charged proteins, mammalian cells, or bacteria such as Staphylococcus aureus …”

Section: Methodsmentioning

confidence: 99%

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Niu

Rivera

et al. 2021

Langmuir

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Motivated by observations of cell orientation at biofilm−substrate interfaces and reports that cell orientation and adhesion play important roles in biofilm evolution and function, we investigated the influence of surface chemistry on the orientation of Escherichia coli cells captured from flow onto surfaces that were cationic, hydrophobic, or anionic. We characterized the initial orientations of nonmotile cells captured from gentle shear relative to the surface and flow directions. The broad distribution of captured cell orientations observed on cationic surfaces suggests that rapid electrostatic attractions of cells to oppositely charged surfaces preserve the instantaneous orientations of cells as they rotate in the near-surface shearing flow. By contrast, on hydrophobic and anionic surfaces, cells were oriented slightly more in the plane of the surface and in the flow direction compared with that on the cationic surface. This suggests slower development of adhesion at hydrophobic and anionic surfaces, allowing cells to tip toward the surface as they adhere. Once cells were captured, the flow was increased by 20-fold. Cells did not reorient substantially on the cationic surface, suggesting a strong cell−surface bonding. By contrast, on hydrophobic and anionic surfaces, increased shear forced cells to tip toward the surface and align in the flow direction, a process that was reversible upon reducing the shear. These findings suggest mechanisms by which surface chemistry may play a role in the evolving structure and function of microbial communities.

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Section: Methodsmentioning

confidence: 99%

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Niu

Rivera

et al. 2021

Langmuir

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“…CO is one of the most common toxic gases and harmful to environment and human health. The elimination of CO and especially the oxidation of CO, a simple and typical reaction in heterogeneous catalysis, has attracted wide attention in recent years due to its academic value and possible applications [9][10][11][12][13]. Progress in nanotechnology provides a precious tool for the study of CO oxidation at the nanoscale.…”

Section: Introductionmentioning

confidence: 99%

Co-Supported CeO2Nanoparticles for CO Catalytic Oxidation: Effects of Different Synthesis Methods on Catalytic Performance

et al. 2020

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Hydrothermal and co-precipitation methods were studied as two different methods for the synthesis of CeO 2 nanocatalysts. Co/CeO 2 catalysts supported by 2, 4, 6, or 8wt% Co were further synthesized through impregnation and the performance of the catalytic oxidation of CO has been investigated. The highest specific surface area and the best catalytic performance was obtained by the catalyst 4wt% Co/CeO 2 with the CeO 2 support synthesized by the hydrothermal method (4% Co/CeO 2 -h), which yielded 100% CO conversion at 130 • C. The formation of CeO 2 nanoparticles was confirmed by TEM analysis. XRD and SEM-EDX mapping analyses indicated that CoO x is highly dispersed on the 4% Co/CeO 2 -h catalyst surface. H 2 -TPR and O 2 -TPD results showed that 4% Co/CeO 2 -h possesses the best redox properties and the highest amount of chemically adsorbed oxygen on its surface among all tested catalysts. Raman and XPS spectra showed strong interactions between highly dispersed Co 2+ active sites and exposed Ce 3+ on the surface of the CeO 2 support, resulting in the formation of the strong redox cycle Ce 4+ + Co 2+ ↔ Ce 3+ + Co 3+ .This may explain that 4% Co/CeO 2 -h exhibited the best catalytic activity among all tested catalysts.

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“…The cationic PLL backbones adsorb to the negatively charged silica, effectively tethering the PEG side chains. The resulting end-tethered layers of PEG chains have been proven, in many previous studies in our lab ,,,− and the laboratories of others, ,, to be exceptionally stable over the conditions and time scales of the experiments, including exposure to varied ionic strengths, pH values, polymer solutions, particles, bacteria, and mammalian cells. In the current studies, no bacterial cells adhered to the coatings, an indication of both the robustness and stability of the PEG layers.…”

Section: Methodsmentioning

confidence: 79%

Escherichia coli Swimming back Toward Stiffer Polyetheylene Glycol Coatings, Increasing Contact in Flow

Shave

Raman

et al. 2021

ACS Appl. Mater. Interfaces

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Bacterial swimming in flow near surfaces is critical to the spread of infection and device colonization. Understanding how material properties affect flagella- and motility-dependent bacteria–surface interactions is a first step in designing new medical devices that mitigate the risk of infection. We report that, on biomaterial coatings such as polyethylene glycol (PEG) hydrogels and end-tethered layers that prevent adhesive bacteria accumulation, the coating mechanics and hydration control the near-surface travel and dynamic surface contact of E. coli cells in gentle shear flow (order 10 s–1). Along relatively stiff (order 1 MPa) PEG hydrogels or end-tethered layers of PEG chains of similar polymer correlation length, run-and-tumble E. coli travel nanometrically close to the coating’s surface in the flow direction in distinguishable runs or “engagements” that persist for several seconds, after which cells leave the interface. The duration of these engagements was greater along stiff hydrogels and end-tethered layers compared with softer, more-hydrated hydrogels. Swimming cells that left stiff hydrogels or end-tethered layers proceeded out to distances of a few microns and then returned to engage the surface again and again, while cells engaging the soft hydrogel tended not to return after leaving. As a result of differences in the duration of engagements and tendency to return to stiff hydrogel and end-tethered layers, swimming E. coli experienced 3 times the integrated dynamic surface contact with stiff coatings compared with softer hydrogels. The striking similarity of swimming behaviors near 16-nm-thick end-tethered layers and 100-μm-thick stiff hydrogels argues that only the outermost several nanometers of a highly hydrated coating influence cell travel. The range of material stiffnesses, cell-surface distance during travel, and time scales of travel compared with run-and-tumble time scales suggests the influence of the coating derives from its interactions with flagella and its potential to alter flagellar bundling. Given that restriction of flagellar rotation is known to trigger increased virulence, bacteria influenced by surfaces in one region may become predisposed to form a biofilm downstream.

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Surfaces that Adhesively Discriminate Breast Epithelial Cell Lines and Lymphocytes in Buffer and Human Breast Milk

Cited by 8 publications

References 35 publications

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Surface Chemistry Guides the Orientations of Adhering E. coli Cells Captured from Flow

Co-Supported CeO2Nanoparticles for CO Catalytic Oxidation: Effects of Different Synthesis Methods on Catalytic Performance

Escherichia coli Swimming back Toward Stiffer Polyetheylene Glycol Coatings, Increasing Contact in Flow

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