Surface ultrastructure of Blastocystis sp. isolated from cattle

Abstract

Abstract. Widisuputri NKA, Lastuti NDR, Suprihati E, Hastutiek P, Plumeriastuti H, Mufasirin, Puspitasari H, Suwanti LT. 2021. Surface ultrastructure of Blastocystis sp. isolated from cattle. Biodiversitas 22: 1514-1518. Blastocystis sp is a protozoan parasite commonly detected in the intestinal tract of humans and various animals that causes zoonotic blastocystosis. The pathogenic potential of Blastocystis is still being evaluated, some Blastocystis sp are completely pathogenic, while others can be considered commensal and hypothetical, related to the role of the surface coat of Blastocystis sp. This study aimed to compare the surface ultrastructure of Blastocystis sp. in cattle with diarrhea and non diarrhea by Scanning Electron Microscope (SEM). Four Blastocystis sp. isolates were selected from the faeces of four positives cattle which consisted of two diarrhea and two non-diarrhea cattle. The result showed that Blastocystis sp. in cattle appeared in round shape and reproduced by binary fission. The surface cell of Blastocystis sp. isolates from diarrhea cattle had a rough surface while organism of non diarrhea cattle isolates was very smooth. Bacteria were seen attached to the surface of Blastocystis sp. from diarrhea cattle faeces. In conclusion, the features of the surface structure of Blastocystis sp. correlated with symptomatic appearance. The surface structure of Blastocystis sp. isolates from cattle with diarrhea was rougher than non diarrhea.

Molecular subtyping and phylogeny of Blastocystis sp. isolated from turkey (Meleagris gallopavo) populations in Penang, Malaysia

“…The surface structure for both isolates of closed house and free-range turkeys were generally spherical to rounded in shape and had a rough surface coat with undulating cell surface whereas some organisms showed gouges or deep furrows similarly indicated in the isolates from diarrhea cattle (Widisuputri et al, 2021). Meanwhile, Cassidy et al (1994) revealed that the surface structure of chicken isolates appeared to be compact with a smooth and undulating cell surface.…”

“…It has also been suggested that the features of the surface structure of Blastocystis sp. maybe correlated with symptomatic appearance (Widisuputri et al, 2021).…”

Wild hosts and microscopic worlds: Investigating the morphology and surface ultrastructure of Blastocystis sp. in avian and non-human primate species

“…The morphology and cell diameter of Blastocystis Despite the lack of an established study on Blastocystis morphology in wild species in Malaysia, this study explores the morphological and surface ultrastructure features of Blastocystis in avian and non-human primate species in Perak, Malaysia. There were few morphological studies on a diverse group of animals in cattle by Widisuputri et al (2021); Highland bull, llama, and camel by Stenzel et al (1993); pig by Cassidy et al (1994); chicken by Stenzel et al (1993), Cassidy et al (1994), Farah Haziqah et al (2014; ostrich by Stenzel et al (1993), Chandrasekaran et al (2014); monkey by Cassidy et al (1994), Stenzel et al (1997); dog by Duda et al (1998), and cockroach by Farah Haziqah et al (2017); while large-billed crow by Yong et al (2008), turkey by Siti Alawiyah et al (2021) have been conducted worldwide.…”

“…To overcome this challenge and gain deeper insights into its structure, scanning electron microscopy (SEM) has emerged as a powerful tool. SEM enables detailed visualizations of the surface ultrastructure, topography, and variation in surface coats of microorganisms (Cassidy et al, 1994;Widisuputri et al, 2021). Previous SEM studies have highlighted variations in the surface coats of Blastocystis isolates from different host species, suggesting potential differences in pathogenic potential (Yason & Tan, 2015;Farah Haziqah et al, 2018a, 2018b.…”

“…across a range of species, including humans (primarily), monkeys, pigs, chickens, ducks, ostriches, dogs, cockroaches, and cattle. These studies have unveiled insights into the surface coat and organelles of Blastocystis (Cassidy et al, 1994;Duda et al, 1998;Lee & Stenzel, 1999;Zhang et al, 2012;Farah Haziqah et al, 2018a, 2018bYason & Tan, 2018;Widisuputri et al, 2021). The absence of studies investigating the surface ultrastructure of Blastocystis in peafowl, pheasant, and lion-headed tamarin leaves a gap in our understanding of this topic…”