Surface Ultrastructural Changes in the Gill and Liver Tissue of Asian Sea Bass Lates calcarifer (Bloch) Exposed to Copper

Paruruckumani, P. S.; Maharajan, A.; Ganapiriya, V.; Narayanaswamy, Y.; Jeyasekar, R. Raja

doi:10.1007/s12011-015-0370-z

Cited by 20 publications

(8 citation statements)

References 50 publications

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“…The SEM micrograph of gill in sodium arsenite treated Channa Punctatus (Figure 9) also reveals swelling and curling of secondary lamellae, complete fusion of secondary lamellae and surface wrinkling in numerous areas of the gill tissue. These observations are in accordance to those reported in Surface ultrastructural changes in the gill and liver tissue of Asian sea bass Lates calcarifes (Bloch) exposed to copper (Paruruckumani et al, 2015)…”

Section: Resultssupporting

confidence: 93%

Histopathological and ultrastructural changes in the gill and liver of fresh water fi sh Channa punctatus exposed to sodium arsenite

Das¹,

Goswami²

2018

Biosci. Biotech. Res. Comm

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Arsenic, is one of the most important and concerned global environmental toxicants. Correlations have been found between chronic arsenic poisoning and many severe health effects including cancers, hypertension and ischemic heart disease etc. However, the proper understanding of the role of arsenic in the cause of these diseases is still limited. In this work, we studied the toxicity effect of sodium arsenite in the gill and liver tissues of fresh water fi sh Channa punctatus and for the fi rst time observed the histopathological as well as surface ultrastructural changes on it. The liver and gill tissues of Channa punctatus were exposed to sub-lethal (12 ppm: parts per million) concentration of sodium arsenite (NaAsO2) for 96 hours. The histopathological effects of sodium arsenite on the liver and gill tissues were studied by light microscopy. The surface ultrastructural changes on the same tissues were investigated by scanning electron microscopy (SEM). The results were compared with the normal structure of liver and the gill tissue of a control group of Channa punctatus. Gill tissues exposed to arsenic showed hyperplasia, desquamation, and necrosis of epithelium, epithelial lifting, oedema, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae. Hepatic lesions in the form of cloudy swelling of hepatocytes, congestion, vacoular degeneration, karyolysis, dilation of sinusoids and nuclear hypertrophy were observed in the liver tissue of the exposed group. Thus it has been shown that sodium arsenite can produce signifi cant damage in the ultrastructure of liver and gill tissues. Also the histological and ultrastructural changes on the liver and the gill tissue indicate that arsenic is biologically reactive and gives rise to acute poisoning.

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Section: Resultssupporting

confidence: 93%

Histopathological and ultrastructural changes in the gill and liver of fresh water fi sh Channa punctatus exposed to sodium arsenite

Das¹,

Goswami²

2018

Biosci. Biotech. Res. Comm

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“…The morphological structure of the gills in fi sh has been demonstrated in previous studies by Scanning electron microscopy [31,32]. Paruruckumani, et al [33] determined the histopathological changes in the liver by SEM method with the detection of 2.5% glutaraldehyde and 1% osmium tetroxide in Lates calcarifer fi sh liver tissue. To demonstrate cell/tissue architectures by SEM in paraffi n block sections, Sawaguchi, et al ( 2018) examined rat organs from 30 μm thick paraffi n blocks on New Silane II-coated microscope slides.…”

Section: Discussionmentioning

confidence: 86%

Examination of Paraffin Sections of Different Rainbow Trout (Oncorhynchus mykiss) Tissues by Light and Scanning Electron Microscope

Demirci¹,

Terzi²,

Kesbiç³

2022

Open J Hepatol

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The current study aimed to highlight histopathological findings in paraffin block sections of the liver, gill kidney, and pyloric cecum of rainbow trout (Oncorhynchus mykiss) by different imagining devices such as Scanning Electron (SEM) and Light Microscope (LM). To determine the performance of different imagining methods two different thickness paraffin sections such as 5 and 15 µm about various rainbow trout tissue were prepared for imagining different devices. That sections were imagined by SEM and LM, both sections including 5 and 15 µm were imagined by SEM while just 5 µm was an image by LM. In LM imagining, it was detected that hydropic degeneration and vacuole formations in the liver hepatocytes of fish, as well as hyperplasia in bile ducts. Lamellar epithelial cell hyperplasia/hypertrophy was mild and histopathological findings such as secondary lamellar elevation and edema were more severe in rainbow trout gills. Glomerular atrophy/hypertrophy was moderately detected in the kidneys and hydropic degeneration of tubular epithelium was more severe. No degeneration or necrosis was observed in the lamina epithelium of the pyloric cecum. In SEM imagining of different thickness paraffin sections, cartilage and secondary lamellar structure in the gills, glomerulus, and Bowman’s capsule structure in the kidneys, and the structure of the pyloric cecum was observed. In the SEM imaging of the paraffin block sections of hepatocytes of the liver, the cell nuclei were determined, and also the grooves in the cytoplasm were thought to be vacuoles. As a consequence, the structural elements of the organ had higher clarity in SEM imaging from paraffin block sections, but the histopathological alterations remained unclear. As a result, SEM imaging of fish tissue is more suited for seeing tissue architecture, although LM imaging is better suited for determining and scoring histopathological variations.

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“…Histopathologically, the disorganization of gill was the most frequently observed lesion in S. javus. It is suggested that such lesions are the consequence of a variety of water pollution problems (Cantanhêde et al, 2014;Paruruckumani et al, 2015). The appearance of lamellar aneurysm was also identified from all individuals.…”

Section: Discussionmentioning

confidence: 99%

The Use of Hematological and Histopathological Biomarkers to Assess the Health of Aquatic Ecosystems in Koh Sichang, Thailand

Sopon¹,

Kettratad²,

Piumsomboon³

et al. 2021

CMUJNS

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Koh Sichang area in Thailand is a sink for a wide variety of contaminants such as heavily polluted water from industry and oil spills. This situation may affect the health status of fish living in the area, but such information remains scarce. In this study, we evaluated the health status of java rabbitfish Siganus javus, an important marine fish in Koh Sichang, using hematological and histopathological biomarkers. All fish samples were collected from the Koh Sichang area during December 2017 and January 2018. Although the salinity, pH, and dissolved oxygen levels at sampling points were all within the normal range, abnormal nuclei were observed in erythrocytes (up to ~6% of all erythrocytes) and in some leucocytes (neutrophil, lymphocyte and monocyte). Visceral organs (gill, kidney and liver) were apparently normal in terms of gross morphology, but a wide variety of the histopathological alterations were found at the microscopic level: epithelial hyperplasia and aneurysm in gills; blood congestion and melanomacrophage centers (MMCs) in the liver; renal degeneration, granuloma and MMCs together with unidentified parasites in kidney. Calculation of semi-quantitative parameters [histological alteration index (HAI) and the average value of alteration (AVA)] demonstrated the highest frequency of histopathological alterations in kidney, suggesting that kidney is a sensitive organ. Overall, our observations suggest that S. javus in Koh Sichang is under the pathological state and warrants conservation efforts. Keywords: Erythrocytes, Fish Health, Hematology, Histopathology, Kidney

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Surface Ultrastructural Changes in the Gill and Liver Tissue of Asian Sea Bass Lates calcarifer (Bloch) Exposed to Copper

Cited by 20 publications

References 50 publications

Histopathological and ultrastructural changes in the gill and liver of fresh water fi sh Channa punctatus exposed to sodium arsenite

Histopathological and ultrastructural changes in the gill and liver of fresh water fi sh Channa punctatus exposed to sodium arsenite

Examination of Paraffin Sections of Different Rainbow Trout (Oncorhynchus mykiss) Tissues by Light and Scanning Electron Microscope

The Use of Hematological and Histopathological Biomarkers to Assess the Health of Aquatic Ecosystems in Koh Sichang, Thailand

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