Surface treatments and cell attachment

Ramsey, W. Scott; Hertl, W.; Nowlan, E D; Binkowski, N. J.

doi:10.1007/bf02618296

Cited by 160 publications

(83 citation statements)

References 7 publications

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“…During the repeated passaging by trypsinization, we used standard tissue culture plastic dishes instead of gelatin-coated plates. The early introduction of the tissue culture plastic dishes probably accelerated selection for MSClike cells because the dishes are pretreated under proprietary conditions to increase oxygenated derivatives on the surface of the plastic and thereby make them more hydrophilic and increase the adherence of vertebrate fibroblasts and similar cells (45). The selection by adherence on the treated plastic also met one of the minimal defining criteria for human MSCs (32).…”

Section: Discussionmentioning

confidence: 99%

MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs

Zhao¹,

Gregory²,

Lee³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

140

139

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Mesenchymal stem or stromal cells (MSCs) have many potential therapeutic applications including therapies for cancers and tissue damages caused by cancers or radical cancer treatments. However, tissue-derived MSCs such as bone marrow MSCs (BM-MSCs) may promote cancer progression and have considerable donor variations and limited expandability. These issues hinder the potential applications of MSCs, especially those in cancer patients. To circumvent these issues, we derived MSCs from transgene-free human induced pluripotent stem cells (iPSCs) efficiently with a modified protocol that eliminated the need of flow cytometric sorting. Our iPSC-derived MSCs were readily expandable, but still underwent senescence after prolonged culture and did not form teratomas. These iPSC-derived MSCs homed to cancers with efficiencies similar to BM-MSCs but were much less prone than BM-MSCs to promote the epithelial-mesenchymal transition, invasion, stemness, and growth of cancer cells. The observations were probably explained by the much lower expression of receptors for interleukin-1 and TGFβ, downstream protumor factors, and hyaluronan and its cofactor TSG6, which all contribute to the protumor effects of BM-MSCs. The data suggest that iPSC-derived MSCs prepared with the modified protocol are a safer and better alternative to BM-MSCs for therapeutic applications in cancer patients. The protocol is scalable and can be used to prepare the large number of cells required for "off-the-shelf" therapies and bioengineering applications.

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Section: Discussionmentioning

confidence: 99%

MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs

Zhao¹,

Gregory²,

Lee³

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

140

139

View full text Add to dashboard Cite

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“…Soon after, a variety of surface-synthesis strategies were explored, ranging from use of liquid-phase chemical oxidants [35,36] to application of gas-discharge treatments [37] now commonly employed in the commercial production of disposable plastic tissue cultureware [38]. Evidence mounted supporting the idea that a particular surface functional group -hydroxyl or carboxyl for example -was particularly stimulatory to cell adhesion and proliferation [39][40][41][42][43] over other functional groups. However, it has proven difficult in subsequent research to clearly separate cause-and-effect in the cell adhesion/proliferation process, especially in the ubiquitous presence of proteins, and by doing so, unambiguously separate surface chemistry from all other influences (such as surface energy/water wettability) [44].…”

Section: Cell Adhesion Theoriesmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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“…, with the requirement that it is "tissue culture" polystyrene (indicating that it has been surface modified, typically through corona discharge or gas-plasma, to promote protein and cell adhesion (31,32)). These reagents are needed for the preparation of SAMs:…”

Section: Methodsmentioning

confidence: 99%

Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

Pannier

Segura

2012

Nanotechnology for Nucleic Acid Delivery

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Gene expression within a cell population can be directly altered through gene delivery approaches. Traditionally for nonviral delivery, plasmids or siRNA molecules, encoding or targeting the gene of interest, are packaged within nanoparticles. These nanoparticles are then delivered to the media surrounding cells seeded onto tissue culture plastic; this technique is termed bolus delivery. Although bolus delivery is widely utilized to screen for efficient delivery vehicles and to study gene function in vitro, this delivery strategy may not result in efficient gene transfer for all cell types or may not identify those delivery vehicles that will be efficient in vivo. Furthermore, bolus delivery cannot be used in applications where patterning of gene expression is needed. In this chapter, we describe methods that incorporate material surfaces (i.e., surface-mediated delivery) or hydrogel scaffolds (i.e., hydrogel-mediated delivery) to efficiently deliver genes. This chapter includes protocols for surface-mediated DNA delivery focusing on the simplest and most effective methods, which include nonspecific immobilization of DNA complexes (both polymer and lipid vectors) onto serum-coated cell culture polystyrene and self-assembled monolayers of alkanethiols on gold. Also, protocols for the encapsulation of DNA/cationic polymer nanoparticles into hydrogel scaffolds are described, including methods for the encapsulation of low amounts of DNA (<0.2 μg/μL) and high amounts of DNA (>0.2 μg/μL) since incorporation of high amounts of DNA poses significant challenges due to aggregation.

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Surface treatments and cell attachment

Cited by 160 publications

References 7 publications

MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs

MSCs derived from iPSCs with a modified protocol are tumor-tropic but have much less potential to promote tumors than bone marrow MSCs

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Surface- and Hydrogel-Mediated Delivery of Nucleic Acid Nanoparticles

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