Surface topography of viable articular cartilage measured with scanning white light interferometry

Shekhawat, Vivek K.; Michel, Laurent; Muehleman, Carol; Wimmer, Markus A.

doi:10.1016/j.joca.2009.03.013

Cited by 38 publications

(32 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Half of the dissected sample was fixed in formalin, processed for paraffin embedding, sliced to a thickness of 5 µm, and exposed to deparaffinization washes in xylene and decreasing concentrations of alcohol. Slides were stained with Safranin-O/fast green to evaluate the presence of matrix proteoglycans 34 and imaged with 4× and 10× objectives. Morphology was assessed using previously reported modified scoring criteria, 35 originally established by Mankin and Lippiello.…”

Section: Tissue Analysis: Tissue Loss Histology and Viabilitymentioning

confidence: 99%

“…With a commercially available live/dead staining kit, samples were incubated in phosphate-buffered saline containing calcein AM and ethidium homodimer-1 for 45 minutes at 37°C (Invitrogen Molecular Probes, Eugene, OR). 34 Samples were imaged using a fluorescence-light microscope (Eclipse TE2000-S; Nikon Instruments Inc., New York, NY), charge-coupled device (CCD) camera (SPOT RT-KE Color 3-Shot, Model 7.3x; Diagnostic Instruments, Sterling Heights, MI) and 5× objective.…”

Section: Tissue Analysis: Tissue Loss Histology and Viabilitymentioning

confidence: 99%

See 1 more Smart Citation

Development of a Cartilage Shear-Damage Model to Investigate the Impact of Surface Injury on Chondrocytes and Extracellular Matrix Wear

et al. 2016

View full text Add to dashboard Cite

Background. Many in vitro damage models investigate progression of cartilage degradation after a supraphysiologic, compressive impact at the surface and do not model shear-induced damage processes. Models also neglect the response to uninterrupted tribological stress after damage. It was hypothesized that shear-induced removal of the superficial zone would accelerate matrix degradation when damage was followed by continued load and articulation. Methods. Bovine cartilage underwent a 5-day test. Shear-damaged samples experienced 2 days of damage induction with articulation against polyethylene and then continued articulation against cartilage (CoC), articulation against metal (MoC), or rest as freeswelling control (FSC). Surface-intact samples were randomized to CoC, MoC, or FSC for the entire 5-day test. Samples were evaluated for chondrocyte viability, GAG (glycosaminoglycan) release (matrix wear surrogate), and histological integrity. Results. Shear induction wore away the superficial zone. Damaged samples began continued articulation with collagen matrix disruption and increased cell death compared to intact samples. In spite of the damaged surface, these samples did not exhibit higher GAG release than intact samples articulating against the same counterface (P = 0.782), contrary to our hypothesis. Differences in GAG release were found to be due to tribological testing against metal (P = 0.003). Conclusion. Shear-induced damage lowers chondrocyte viability and affects extracellular matrix integrity. Continued motion of either cartilage or metal against damaged surfaces did not increase wear compared with intact samples. We conjecture that favorable reorganization of the surface collagen fibers during articulation protected the underlying matrix. This finding suggests a potential window for clinical interventions to slow matrix degradation after traumatic incidents.

show abstract

Section: Tissue Analysis: Tissue Loss Histology and Viabilitymentioning

confidence: 99%

Section: Tissue Analysis: Tissue Loss Histology and Viabilitymentioning

confidence: 99%

Development of a Cartilage Shear-Damage Model to Investigate the Impact of Surface Injury on Chondrocytes and Extracellular Matrix Wear

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Reported surface roughness data of healthy and osteoarthritic cartilage surfaces exhibited differences in these studies. For instance, the measurements showed that the mean surface roughness values of smooth, intermediate, and rough cartilage samples were 7.973.3, 29.177.8 and 49.1 μm, respectively, when using high frequency ultrasound [54], while using scanning white light interferometry, the average surface roughness values of human cartilage samples in OA grades 0, 1 and 2 were 0.8070.3, 1.070.3 and 1.7070.9 μm, respectively [56]. Surface roughness was reported to be 30 μm for a healthy condyle with a smooth surface [55].…”

Section: Surface Morphological Properties Of Articular Cartilage and mentioning

confidence: 98%

“…Cartilage surface alterations of animal samples were investigated at a micrometer scale using high frequency ultrasound [54], optical profilometry [55], scanning white light interferometry [56], and laser scanning confocal microscopy (LSCM) [57,58]. Graindorge et al found that changes occurred in the surface morphology of the articular cartilage of sheep leg during wear [38].…”

Section: Surface Morphological Properties Of Articular Cartilage and mentioning

confidence: 99%

Wear in human knees

Wang

Peng

2015

Biosurface and Biotribology

View full text Add to dashboard Cite

Wear occurs in natural knee joints and plays a pivotal factor in causing articular cartilage degradation in osteoarthritis (OA) processes. Wear particles are produced in the wear process and get involved in inflammation of human knees. This review presents progresses in the mechanical and surface morphological studies of articular cartilages, wear particles analysis techniques for wear studies and investigations of human knee synovial fluid in wear of human knees. Future work is also included for further understanding of OA symptoms and their relations which may shed light on OA causes. & 2015 Southwest Jiaotong University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

show abstract

“…Prior to measurements, the surface was blotted with low lint wipes, sprayed with canned air for 5 s, and air dried for 1-2 min at room temperature in order to avoid interference of discrete fluid films with the interferometry measurements. 22 After testing, Indian ink was used to visualize the damaged cartilage tissue on the articular surface and imaged with a dissection microscope (Nikon SM2800; Melville, NY). The areas that were stained after wiping the surface with a damp cloth indicated damage based on the adherence of Indian ink to fibrillated cartilage, and were assessed with a semi-quantitative analysis.…”

Section: Wear Assessmentmentioning

confidence: 99%

Methods to assess in vitro wear of articular cartilage

McGann

Vahdati

Wagner

2012

Proc Inst Mech Eng H

View full text Add to dashboard Cite

New orthopedic implants for focal cartilage defects replace only a portion of the articulating joint and wear against the opposing cartilage surface. The objective of this study was to investigate different methodologies to quantify cartilage wear for future use in screening potential implant materials and finishes. In determining the optimal test parameters, two different cartilage surface geometries were compared: smaller specimens had a flat surface, while larger ones made contact in the center but not at the edge owing to the curvature of the articulating surface. The cartilage wear of the two geometries was compared using three different techniques: the collagen worn from the cartilage specimens was assessed with a modified wear factor, the surface damage was made visible with Indian ink and was quantified, and the change in surface roughness was measured. To interpret the experimental results, maximum shear stresses were evaluated with sliding contact finite element models. Although the modified wear factor was considered to be the most accurate assessment of cartilage wear, surface damage was an effective, inexpensive, and quick technique to evaluate potential implant materials. Flat specimens showed excessive wear at the edges owing to a non-physiologic stress concentration, while the larger specimens wore more uniformly across the surface. These results will be applied to future studies evaluating prospective implant materials.

show abstract

Surface topography of viable articular cartilage measured with scanning white light interferometry

Cited by 38 publications

References 25 publications

Development of a Cartilage Shear-Damage Model to Investigate the Impact of Surface Injury on Chondrocytes and Extracellular Matrix Wear

Development of a Cartilage Shear-Damage Model to Investigate the Impact of Surface Injury on Chondrocytes and Extracellular Matrix Wear

Wear in human knees

Methods to assess in vitro wear of articular cartilage

Contact Info

Product

Resources

About