Surface topography and ultrastructural architecture of the tegument of adult Carmyerius spatiosus Brandes, 1898

Anuracpreeda, Panat; Phutong, Sumittra; Ngamniyom, Arin; Panyarachun, Busaba; Sobhon, Prasert

doi:10.1016/j.actatropica.2014.12.003

Cited by 15 publications

(27 citation statements)

References 39 publications

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“…Collection of adult parasites was done according to the method described by Anuracpreeda et al . (2015) and Panyarachun et al . (2013).…”

Section: Methodsmentioning

confidence: 96%

“…Large numbers of immature parasites cause severe acute gastroenteritis, dehydration, maldigestion and death, particularly in young animals (Ilha et al 2005; Khan et al 2008). In addition, paramphistomosis in domesticated animals causing economic losses with morbidity and mortality rates as high as 80–90% in livestock industry (Prasitirat et al 1977 a , b ; Gupta et al 1978; Hanna et al 1988; Khan et al 2008; Tariq et al 2008; Anuracpreeda et al 2015). It was reported that the outbreaks of clinical paramphistomosis caused by immature flukes in ruminants are not diagnosed and subclinical infection often passes undiagnosed.…”

Section: Introductionmentioning

confidence: 99%

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The anthelmintic effects of the ethanol extract ofTerminalia catappaL. leaves against the ruminant gut parasite,Fischoederius cobboldi

et al. 2016

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Presently, no effective anthelmintic drugs have been used to treat and control paramphistomosis, a severe disease of ruminants. In this study, we have investigated the in vitro anthelmintic effect of the leaves of Terminalia catappa L. crude extract (TcCE) and albendazole (ABZ) on adult Fischoederius cobboldi after incubating the flukes in RPMI-1640 medium containing the TcCE at various doses and times. The TcCE-treated flukes at all dosages exhibited rapid decrease of motility, and the relative motility (RM) values were decreased sharply from start to 3 h. Worms were killed after 6 and 12 h of treatment with 1000, 1500 and 2000 µg mL(-1) as well as 500 µg mL(-1) of TcCE, respectively. By light microscopy examination, the flukes exhibited the earliest alteration in a limited area of the tegument. At scanning electron microscopy level, the flukes' tegument showed similar sequence of morphological alterations after treatment with ABZ and TcCE that consisted of swelling of ridges and folds, followed by blebbing and rupturing of the blebs, leading to the erosion, lesion and disruption of the tegument. Hence, in vivo studies should be performed to examine whether the TcCE may serve as a powerful anthelmintic drug for treatment of paramphistomosis.

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“…Collection of adult parasites was done according to the method described by Anuracpreeda et al . (2015) and Panyarachun et al . (2013).…”

Section: Methodsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The anthelmintic effects of the ethanol extract ofTerminalia catappaL. leaves against the ruminant gut parasite,Fischoederius cobboldi

et al. 2016

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“…After 4 weeks of infection, the adult worms were collected by teasing the liver apart. All parasite samples were washed several times with Hank’s balance salt (HBS) solution before being processed for further experiments [ 23 ].…”

Section: Methodsmentioning

confidence: 99%

Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease

2016

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BackgroundTropical fasciolosis caused by Fasciola gigantica infection is one of the major diseases infecting ruminants in the tropical regions of Africa and Asia including Thailand. Parasitological diagnosis of fasciolosis is often unreliable and possesses low sensitivity. Therefore, the detection of circulating parasite antigens is thought to be a better alternative for diagnosis of fasciolosis, as it reflects the real parasite burden.MethodsIn this study, we have produced a monoclonal antibody (MoAb) against recombinant F. gigantica cathepsin L1 (rFgCatL1), and developed both sandwich enzyme-linked immunosorbent assay (sandwich ELISA) and immunochromatographic (IC) test for rapid detection of circulating cathepsin L1 protease (CatL1) in the sera from mice experimentally and cattle naturally infected with Fasciola gigantica. MoAb 4E3 and biotinylated rabbit anti-recombinant CatL1 antibody were selected due to their high reactivities and specificities.ResultsThe lower detection limits of sandwich ELISA and IC test were 3 pg/ml and 0.256 ng/ml, respectively. Sandwich ELISA and IC test could detect F. gigantica infection from day 1 to 35 post infection. In experimental mice, the sensitivity, specificity and accuracy were 95%, 100% and 98.6% (for sandwich ELISA), and 93%, 100% and 98.2% (for IC test), while in natural cattle they were 98.3%, 100% and 99.5% (for sandwich ELISA), and 96.7%, 100% and 99.1% (for IC test).ConclusionsThese two assay methods showed high efficiencies and precisions for diagnosis of fasciolosis by F. gigantica.

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“…Active adult P. gracile was collected from the rumen of infected cattle slaughtered for consumption at the local abattoir, Pathum Thani Province, Thailand. They were then washed several times with 0·85% NaCl solution and used immediately .…”

Section: Methodsmentioning

confidence: 99%

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

Anuracpreeda

Chawengkirttikul

Sobhon

2016

Parasite Immunology

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An antigenic component of adult Paramphistomum gracile was characterized by means of indirect enzyme-linked immunosorbent assay (indirect ELISA), sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting using sera from cattle naturally infected with P. gracile, Eurytrema pancreaticum, Fasciola gigantica, Moniezia benedeni, strongylids, Trichuris sp. and Strongyloides sp. The whole body (WB) extracts of P. gracile were fractionated by gel filtration chromatography in a Sephadex G-200 column. It was found that the WB extract fractions, F1-F3 were highly antigenic, F5 was moderately antigenic and F4 was poorly antigenic. For SDS-PAGE and immunoblotting, the antigenic molecules of WB extract and all five fractions were mostly at molecular weights (MW) ranging from 12 to 150 kDa. One antigenic protein of 16 kDa detected in WB extract and F1-F3 was found to give a consistent reaction with sera from infected cattle. The antigenicity of the purified 16 kDa protein was confirmed by immunoblotting and indirect ELISA using a pool of sera and individual serum samples from infected cattle (at 1 : 78 125 dilution) and hyperimmunized rabbit (at 1 : 390 625 dilution). This finding suggests that the 16 kDa protein may be a potential antigen for the immunodiagnosis of cattle paramphistomosis caused by P. gracile.

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Surface topography and ultrastructural architecture of the tegument of adult Carmyerius spatiosus Brandes, 1898

Cited by 15 publications

References 39 publications

The anthelmintic effects of the ethanol extract ofTerminalia catappaL. leaves against the ruminant gut parasite,Fischoederius cobboldi

The anthelmintic effects of the ethanol extract ofTerminalia catappaL. leaves against the ruminant gut parasite,Fischoederius cobboldi

Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease

Antigenic profile, isolation and characterization of whole body extract of Paramphistomum gracile

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