Surface structures of the gonococcus.

Grimble, A.; Armitage, L. R. G.

doi:10.1136/sti.50.5.354

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…This interesting finding could mean that heating altered the cell-wall stability of colonial type 4 gonococci in such a way as to release endotoxin more easily and make it available to cause the same effect on chicken embryos as was found with the unheated and heated cells of the virulent types. Electronmicroscopic examination of heated and unheated cells of the three colonial types of gonococci revealed that unheated cells of virulent colonial types 1 and 5 carry small blebs of endotoxin which confirmed the findings of Grimble and Armitage (1974). The heated cells of all three colonial types released endotoxin.…”

Section: Discussionsupporting

confidence: 61%

Role of Endotoxin in the Pathogenicity of Neisseria Gonorrhoeae Colonial Types 1, 4 and 5 Determined by Chicken Embryo Model

Hafiz

1986

Journal of Medical Microbiology

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Summary. The pathogenicity of Neisseria gonorrhoeae is a subject of considerable interest. It is believed that N . gonorrhoeae of colonial type 1 are pathogenic while those of type 4 are not. This is based on experimentation in human volunteers. The object of this study was to determine the reasons for the differences of susceptibility of chicken embryos to N . gonorrhoeae strains of colonial types 1 , 4 , 5 and 1 R (a type-1 revertant from a non-pathogenic type 4 strain originally tested in human volunteers). Colonial types 1, 5, 1 R and 4 caused mortality rates of 80, 70, 85 and 20% respectively. This variation in lethality appeared to depend upon the availability of free extra-cellular endotoxin and this was confirmed by chicken-embryo inoculation results and electronmicroscopy of normal and heated colonial types 1,4 and 5. Similar results were obtained by inoculating purified endotoxins from these types into chicken embryos. The results of this study suggest that endotoxins play a major role in the pathogenicity of N . gonorrhoeae and that the variations in virulence of the colonial types depends on the stability of their cell walls.

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Section: Discussionsupporting

confidence: 61%

Role of Endotoxin in the Pathogenicity of Neisseria Gonorrhoeae Colonial Types 1, 4 and 5 Determined by Chicken Embryo Model

Hafiz

1986

Journal of Medical Microbiology

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“…Similarly, in studies with perfused preparations of isolated human fallopian tube, pilate type-1 gonococci grown in vitro adhered (Ward, Watt and Robertson, 1974), but other studies demonstrated that non-pilate organisms also attached to fallopian tube held in organ culture (Taylor-Robinson et al, 1974). Anti-pili antibodies occur in natural infection (Buchanan et al, 1973) but pili comparable to those seen in vitro are rarely seen on gonococci in infected urethral exudates (Novotny, Short and Walker, 1975), although larger appendages have been described (Grimble and Armitage, 1974). Thus, although some of the present evidence suggests that pili may be involved in adherence to the urogenital tract at an early stage in the infective process, this does not seem to be the only factor concerned.…”

mentioning

confidence: 99%

The adherence of pilate and non-pilate strains of neisseria gonorrhoeae to human and guinea-pig epithelial tissues

Tebbutt¹,

Veale

Hutchison³

et al. 1976

Journal of Medical Microbiology

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“…Isolated blebs and possible membranous extensions of different sizes and shapes were seen in many micrographs (Figs 2, 4a, and 10). Other investigators have described similar structures Grimble and Armitage, 1974;DeVoe and Gilchrist, 1975;McGee et al, 1976), and lipopolysaccharides were isolated from comparable blebs . These blebs and membrane extensions were present on the external surface of the cell wall and occasionally wrapped around the cell (Fig.…”

Section: Attachment To Spermmentioning

confidence: 61%

Attachment of Neisseria gonorrhoeae to human sperm. Microscopical study of trypsin and iron.

Gomez¹,

Stenback²,

James³

et al. 1979

Sexually Transmitted Infections

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SUMMARY Pilated Neisseria gonorrhoeae of colony type 1 (TI) and non-pilated bacteria of colony type 4 (T4) were observed by transmission (TEM) and scanning electron microscopy (SEM). No pili were observed on T4 gonococci, but two types of pili-straight, type a, and bent, type b-were seen on TI by TEM. When incubated with human sperm and examined by either TEM or SEM, Ti gonococci were seen to attach by individual pili, by several pili wound together as a rope, or by direct contact. Gonococci from T4 colonies attached only by direct contact. Treatment with typsin (I mg/ml) damaged or removed pili from gonococci. After incubation with trypsin, attachment of pilated gonococci to sperm was decreased significantly, but such treatment did not affect attachment of non-pilated gonococci. Incubation of gonococci from either colony type in 0 1 mmol/l ferric nitrate, followed by incubation with sperm, significantly increased attachment of only T4 bacteria. No pili were seen on T4 gonococci treated with ferric ntrate; thus, it appears that factors other than pili alone are concerned in attachment of these gonococci to sperm.

show abstract

Surface structures of the gonococcus.

Cited by 5 publications

References 7 publications

Role of Endotoxin in the Pathogenicity of Neisseria Gonorrhoeae Colonial Types 1, 4 and 5 Determined by Chicken Embryo Model

Role of Endotoxin in the Pathogenicity of Neisseria Gonorrhoeae Colonial Types 1, 4 and 5 Determined by Chicken Embryo Model

The adherence of pilate and non-pilate strains of neisseria gonorrhoeae to human and guinea-pig epithelial tissues

Attachment of Neisseria gonorrhoeae to human sperm. Microscopical study of trypsin and iron.

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