Surface Sterilization of Nodules with Calcium Hypochlorite<sup>1</sup>

Wilson, Jack; Punyasingha, T.

doi:10.2134/agronj1939.00021962003100120003x

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Other Surface Sterilization Protocols1

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2022

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“…In addition to the common surface sterilization using ethanol and NaOCl, a few other methods are also reported. A combination of 3.15% calcium hypochlorite along with sterilant mentioned earlier was also used for surface sterilant [130]. Waheeda and Shyam [69] evaluated 3.15% calcium hypochlorite-10% sodium hydrogen carbonate-1% sodium azide as the most efficient surface sterilization method, as compared to three conventional methods using 70% ethanol, cyclohexamide-3.15% NaOCl, and 70% ethanol-0.9% NaOCl-10% sodium hydrogen carbonate.…”

Section: Other Surface Sterilization Protocolsmentioning

confidence: 99%

Surface sterilization for isolation of endophytes: Ensuring what (not) to grow

et al. 2022

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Endophytic microbiota opens a magnificent arena of metabolites that served as a potential source of medicines for treating a variety of ailments and having prospective uses in agriculture, food, cosmetics, and many more. There are umpteen reports of endophytes improving the growth and tolerance of plants. In addition, endophytes from lifesaving drug-producing plants such as Taxus, Nothapodytes, Catharanthus, and so forth have the ability to produce host mimicking compounds. To harness these benefits, it is imperative to isolate the true endophytes, not the surface microflora. The foremost step in endophyte

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Section: Other Surface Sterilization Protocolsmentioning

confidence: 99%