“…For example, chymotrypsin used for digestive purposes, superoxide dismutase loaded as an antioxidant in ophthalmology, lysozyme/bovine serum albuminencapsulated microparticles, lactoferrin for antitumor activity, β-lactamase for increased thrombin cleavage, and alkaline phosphatase for acceleration of ossification have been developed as promising carriers for a specific protein or enzyme drug delivery system. [33][34][35][36][37][38] Hence, it is believed that the use of proteins in CaCO 3 microspheres developed via biomineralization eliminates the limitations of conventional methods while simultaneously providing the options to encapsulate both hydrophilic and hydrophobic molecules or different molecule complexes. 39 Lysosomes are membrane-bound organelles that contain up to 40 acidic enzymes and are small vesicles composed of a plasma membrane that can prevent aggressive enzymes from destroying cellular structures.…”